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We narrowed to 893 results for: plasmid

Showing: 541 - 560 of 893 results

  1. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...the assay multiple plasmids containing ITR were tested. Plasmid #59462 is one plasmid that gave reliable... calculated using this plasmid, but will change if you use a different plasmid. Sample Calculation Size...Size in bp of Addgene plasmid #59462 : 6208 bp Concentration of Addgene plasmid #59462 : 1.07 μg/μL Molecular...consistent results. Use the recommended plasmid, or test multiple plasmids to find a suitable one. Some labs...ITR primer, 5'-CGGCCTCAGTGAGCGA ITR-containing plasmid for standard curve RNase-free DNase 10X DNase buffer...Nuclease Free Water 4.7 μL 470 μL Procedure Prepare a plasmid stock of 2 x 10 9 molecules/μL to generate a standard...standard curve: One option is to use plasmid #59462 from Addgene. The values highlighted below in red ...

  3. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ....  Table 1: Monomeric FP Timers and Associated Plasmids from Subach et al. Protein Excitation (nm) ...) Emission (nm) Brightness pKa Maturation Plasmids Fast-FT 403 (blue), 583 (red) 466 (blue), 606 (red...

  7. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...experiment. Also learn about Addgene’s knock-in plasmids for reagents to help you knock your knock-in experiment...Cells Resources on Addgene.org Browse All CRISPR Plasmids Find Validated gRNAs for Your Next Experiment ...

  8. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ... development includes everything from tracking plasmids from the moment a deposit kit is sent to a depositor...improvements, you are benefited with reassurance your plasmids will not get lost in a freezer and your viral ...

  9. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...used pairs to measure FRET. The table below lists plasmids that can be used to create your choice of fluorescent... fusion protein with your gene of interest: Plasmid Color Expression Description pPROEX Aqua Cyan...

  10. New Viral Vectors - Spring 2025

    Type
    Blog Post
    ...service, which offers over 3,000 eligible transfer plasmids and five different serotypes. Not sure what to...

  15. Molecular Biology Protocol - Restriction Digest of Plasmid DNA

    Type
    Protocol
    ... Protocols Restriction Digest of Plasmid DNA Restriction Digest of Plasmid DNA You ...digest of plasmid DNA Protocols...You may also like... Recovering Plasmid DNA from Bacterial Culture Purifying DNA from an Agarose Gel DNA...is also used to quickly check the identity of a plasmid by diagnostic digest . Last Upload: Oct. 11th, ...chamber Pipetman Reagents Liquid DNA aliquot of your plasmid of interest (see below for recommend amounts) Appropriate...Procedure Select restriction enzymes to digest your plasmid. Pro-Tip To determine which restriction enzymes... it isn't methylation sensitive . Plasmids grown in Dam or Dcm methylase positive strains...

  16. Protocol - How to Streak a Plate

    Type
    Protocol
    ...Agar Plates Bacterial Transformation Recovering Plasmid DNA from Bacterial Culture Introduction If you ...or stab culture of bacteria and need to purify plasmid DNA from it, you will want to isolate an individual...will minimize the chance of having a mixture of plasmids in your purified DNA. This protocol explains how...antibiotic. Label the bottom of the plate with the plasmid name and the date. It is also a good idea to add...overnight (12-18 hours) at 37 °C. Pro-Tip Some plasmids or bacteria need to be grown at 30 °C instead ... 37 °C. This is often true for large unstable plasmids, which sometimes recombine at 37 °C. Be sure to...single colonies, you can proceed to Recovering Plasmid DNA or use the individual colonies for other experiments...

  17. Gibson Assembly Protocol

    Type
    Protocol
    ...Design your plasmid and order primers (see figure to the right). When designing your plasmid, think about...correct plasmid product by restriction digest . Sequence the important regions of your final plasmid, particularly...efficient and effective method for the assembly of plasmids, and molecular biologists now use this method ...segments you will need to join to create your final plasmid. Adjacent segments should have identical sequences... background and enrich for correctly assembled plasmids by designing primers to split an antibiotic resistance...reaction if you're only making a small change in a plasmid (such as point mutations). Generate DNA segments...

  19. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ...How to Ligate Plasmid DNA... Protocols DNA Ligation DNA Ligation You may also like... Plasmid Cloning by Restriction...Restriction Enzyme Digest Restriction Digest of Plasmid DNA Bacterial Transformation Background Information...final step in the construction of a recombinant plasmid is connecting the insert DNA (gene or fragment ...physically attached to the backbone and the complete plasmid can be transformed into bacterial cells for propagation... and verifies the antibiotic resistance of the plasmid Cut vector - Background due to uncut vector Cut... Any colonies indicate contamination of intact plasmid in ligation or transformation reagents Optimizing...
Showing: 541 - 560 of 893 results