We narrowed to 40 results for: plasmid

How to purify plasmid DNA from a bacterial culture, including protocols, tips, and FAQ

Protocols for plasmid cloning by PCR.

Protocol - How to Ligate Plasmid DNA

Information about plasmid cloning by restriction enzyme digest (subcloning), including design and experimental procedures.

Modify your plasmid MCS or add short sequences using oligo overlap cloning.

A wide variety of protocols from Addgene that can be used for basic molecular biology, plasmid cloning, and titering and testing your viral preparations.

Protocol, tips, and FAQ for how to perform a restriction digest of plasmid DNA

Protocol for how to perform a diagnostic digest to verify plasmid size, verify insert orientation, and more.

Protocol for Inoculating a Bacterial Culture

Learn how to transform E. coli with your plasmid of interest.

Protocol for creating a glycerol stock, including additional tips for long-term storage.

Best practices for performing sequence analysis of a plasmid

Use this protocol to generate lentivirus

Follow this protocol to perform amplification of CRISPR pooled plasmid libraries in Escherichia coli cells.

Determine the number of genome-containing particles in an AAV prep using qPCR, SYBR Green Technology, and Addgene’s AAV Titration protocol.

Learn how to streak bacteria on an LB agar plate to obtain single colonies.

Get started with Gibson Assembly Cloning!

A collection of Addgene's video content, including how-to screen captures, lab procedure protocols, and career advice videos.

Addgene's protocol for using the pLKO.1 cloning vector, a backbone used by the RNAi consortium for targeting human and mouse genes.

Learn how to add antibiotic to a plate after it's already been poured and solidified.