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We narrowed to 49 results for: snap tag

Showing: 41 - 49 of 49 results

  1. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...contains 24 copies of SunTag, a synthetic scaffold that can recruit GFP fused to the SunTag-specific antibody...biochemical method developed in 2009, takes a “snapshot” of all of the mRNA bound-ribosomes in a cell....Imaging by Coat protein Knock-off) instead takes advantage of the ribosome’s movement. When the ribosome ...indicating translational repression. During later stages, single RFP-labeled osk and Oskar protein were ...scFv. Each time the reporter is translated, the SunTag sites on the nascent polypeptide recruit scFV-GFP..., as the authors of these papers did. Since the SunTag-based system tethers mRNAs to the plasma membrane...Additional Resources the Addgene Blog Learn More about SunTag Catch up on Your GFP Background Choose the Brightest...

  2. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ...newly synthesized product from a site-directed mutagenesis experiment? The answer to both questions is the...to methylation if the enzyme's recognition site (TCTAGA) is preceded by GA or followed by TC. As shown ...cleave DNA. DpnI is often used for site directed mutagenesis. During this process, incorporation of a desired...illustrates how sequence visualization software (we used Snapgene here) can help you assess whether your restriction...Troubleshoot Your Sequencing Results Use REPLACR Mutagenesis to Mutate Your Plasmids Resources on Addgene.org...

  3. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    ...three larval stages where larvae molt in order to continue growing in the next larval stage. After the ...the third larval stage, Drosophila will pupate, which takes about one week from the initial deposition of...through their translucent bodies. This is the ideal stage to select for females because you can be certain...Drosophila cell lines are extremely effective. Their Snapdragon tool helps you select primers to synthesize RNA...

  4. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...and in more than 30 countries around the world. Snapshots of the BioBuilder experience With its intentionally...recognized platform in March to exhibit projects at any stage of completion, from pitch to prototype. Last year...preparing local (Massachusetts), economically disadvantaged students for paid summer internships. A summer...

  5. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...more about the Gateway cloning method and its advantages. An introduction to Gateway technology The Gateway..., etc). This video demonstrates how to use the Snapgene program to design Gateway plasmids.    ...and verify that your construct is functional. Advantages of the Gateway cloning method Compatibility and...JY, Chin CF (2015) Gateway Cloning Technology: Advantages and Drawbacks. Clon Transgen 4:138. doi:10.4172...

  6. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...possible consequences of FP aggregation, as Erik Snapp’s blog post shows. To avoid these issues, Subach ...knowledge of protein structure and saturation mutagenesis. mK-GO matures from green to red, and the FT ...absorption spectra). Subach et al’s directed mutagenesis also produced the three separate timers shown...

  7. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    ...lineage, without dedifferentiating into a pluripotent stage. Cellular reprogramming The reversal of a differentiated...reprogramming [1]. The process can be divided into two stages: Dedifferentiation - Conversion of adult somatic...differentiated cells of choice. The dedifferentiation stage involves overexpression of four reprogramming factors...differentiated somatic cell to revert back to a pluripotent stage (iPSC formation) [2, 3]. The iPSCs then proliferate...reprogrammed to iPSCs successfully [10-12]. The next stage of reprogramming consists of redifferentiation of... resources More (due to additional pluripotency stage) Less Safety Less (C-MYC, a known oncogene is ...Williams, L.A., B.N. Davis-Dusenbery, and K.C. Eggan, SnapShot: directed differentiation of pluripotent stem ...

  9. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...tendency – mean, median, and mode give you a summary snapshot without telling you anything about outliers or...dependent variables worked synergistically, antagonistically, were additive, etc.). ANCOVA and MANCOVA...
Showing: 41 - 49 of 49 results