1,191 results

This post describes evoCAST, a new CRISPR-associated transposase tools optimized for genetic editing in human cells.

Check out the latest Hot Plasmids, viral vectors, and antibodies in the Addgene repository!

Learn how to select the plasmid purification kit that best suits your needs!

This post describes the design of shuttle vectors, which are designed for replication, selection, and expression in more than one host species.

This post describes the biology and use of integrase-deficient lentiviral vectors in comparison to standard lentiviral vectors.

Learn about Addgene's QC process and how and why we maintain a high standard of sequence accuracy across our plasmid collection.

Addgene adds new ready-to-use viral vectors to our service every month. Read all about the latest additions here!

Addgene remains steadfast in our mission to accelerate research and discovery by improving access to high-quality scientific materials and information. Our agility as a nonprofit resource can prove to be vital during this period of constrained funding.

This post covers loading controls and normalization of semi-quantitative western blots. We cover housekeeping proteins and total protein loading controls.

This post describes CRISPR-derived base editors that catalyze base transversions, such as purines like A or G to pyrimidines like T or C.

This post describes CRISPR-derived base editors to convert cytosine to adenine or guanine, called CGBEs or GBEs.

Learn about how CRISPR systems are classified. Addgene's guide to typing CRISPR.

Check out the latest Hot Plasmids, viral vectors, and antibodies in the Addgene repository!

Addgene Executive Director Chonnettia Jones reflects on highlights of 2024 and shares exciting plans for 2025.

An overview of cytosine and adenine base transition editors, which make precise changes in DNA bases without causing double-strand breaks.

Learn about how Cascade-Cas3 can be used as a CRISPR tool to degrade large fragments of DNA.

Changes in export control regulations have make rabies and VSV-G plasmids more accessible to researchers around the world.

Learn about the advantages of using AAV as a delivery method for CRISPR screens.

Addgene adds new ready-to-use viral vectors to our service every month. Read all about the latest additions here!

This post provides tips for designing a prime editing experiment, from pegRNA design, PE tool selection, and choice of DNA sequence to edit.