Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 957 results for: Cre

Showing: 681 - 700 of 957 results

  1. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    ...Scientists continue to engineer opsins to operate at increasingly faster kinetics. The opsin ChETA, for example...regions of a neuron. Optogenetic approaches excel at discrete cell or tissue-subregion targeting down to the...

  3. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ... to help you do better research. CRISPR is an incredibly fast-moving field, and we want to make it easy...Chavez) Day 11: Pooled libraries: Genome-Wide Screening Using CRISPR/Cas9 and Plasmids 101: NGS Quality...

  5. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...submicromolar) DA affinity and high-magnitude responses (increases in fluorescence) at low concentrations (e.g., ...The GRABDA dopamine sensor Yulong Li’s lab has created the GRABDA sensor based on a similar strategy: ...

  7. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...highlighted back in 2015, where sdAbs were used to create GFP scaffolds for transcriptional activation.  ... mouse-derived single-domain antibodies. Image created in BioRender and adapted from Xu, et al., 2021....

  8. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    ...accurately reflect our culture and outlook: Addgene increases productivity by providing world-wide access to...leader in scientific sharing, leading by example to increase sharing in the community Addgene fosters a culture...

  9. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    ...leader’s job to provide the driving inspiration, to create a productive culture and to correct systemic problems...try operating in a different role?  I think the secret sauce can be summed up in one word – initiative...

  10. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ... bacteria and a mammalian selection cassette to screen for positive transfected cells. The addition of...your applications and experimental design. You can create a new strain with a disrupted gene by transforming...

  11. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    ...out-of-the-box ideas that might jump start your learning. 1. Create a peer mentoring group We don’t all get the mentoring...that there’s some correct level of intimacy that creates the magic of a safe space. No matter why, now is...

  15. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...empirical essays had also stirred some of my more creative faculties into motion, such that a desire to once...filmmaking, podcasting, philosophy of science, creative writing, designing and curating exhibitions, animation...

  16. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...different biochemical secondary energy sources to increase protein yields from 36 μg/mL to 266 μg/mL. The...oxytetracycline enzymes. Next, this system was used to recreate natural biosynthetic pathways. Biosynthetic pathways...

  18. Plasmid Cloning by PCR

    Type
    Blog Post
    ...temperatures, but you may need to increase your primer length and increase the Tm if you are trying to clone...

  20. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    ...her mentoring style. Set goals with a mentor: The secret of accountability To make a mentoring relationship...perspectives Develop coaching skills Encourage your creative thinking Enhance leadership skills Support inclusion...
Showing: 681 - 700 of 957 results