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TypeBlog Post...synthetic chloralkane (CA) ligand. The current standard version known as HaloTag is actually HaloTag7,...Organic dyes are typically both brighter and more photostable than fluorescent proteins (often up to 10-fold.... Scale bar, 2 μm. Inset shows comparison with standard confocal microscopy. Image adapted from (Bottanelli...fluorophore’s microenvironment to enhance brightness, stability, or other properties is an active area of research...
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Custom CRISPR Screens & the Green Listed Software
TypeBlog Post... differentially expressed genes can be the next stage in making sense of such data. These targeted screens... gRNAs that target your list of selected genes. Start by choosing which “Reference Library” you’d like...to each reference library before using them to understand how they are set up. Using the “User Upload” ...PubMed Central PMCID: PMC4559080. 5. Tzelepis, Konstantinos, et al. "A CRISPR dropout screen identifies ... -
CRISPR 101 eBook...en español
TypeBlog Post...español! Esta traducción fue regalada a Addgene por Daniel Mejías Sánchez, ¡y no podríamos estar más emocionados...científica profesional, L. Denise Roche, para editarla. Estamos orgullosos de anunciar que CRISPR 101: Una herramienta... -
Science Guides
TypeGuide...Addgene has created the following guides to help you start learning about various scientific concepts related...control molecular signals and cells in order to understand their functions. Read More Pooled Libraries Pooled... -
Addgene's eBook Collection
TypeGuide...When you're not sure where to start or need a handy reference, Addgene's eBooks are here to help!... eBook Collection When you're not sure where to start or need a handy reference, Addgene's eBooks are ... -
5 Science Rap Videos & Their Creator: Tom McFadden
TypeBlog Post...American woman whose cervical cancer cells became a mainstain of biological study without her or her family'...Describes fossil rocks and their importance in understanding biological history. 4. Please Don't Kill ...ways to implement the next generation science standards and communicate science. Learn more about Tom ... -
Addgene Protocols Go Viral: AAV Purification and Titration
TypeBlog Post...generate a standard curve, visit the AAV Titration by qPCR written protocol page. Getting started with tissue...video for some tips and best practices for getting started with tissue cultures. To keep up on all of the... -
Depositor Week 2020 is Happening Now. Join us!
TypeBlog Post...helped scientists around the world quickly get started with COVID-19 research (check out this article ... Here's the schedule for the week: Monday: Fun stats and trivia begins! Tuesday: Depositor features (...from 49 countries* and reached 102 countries. We started from 474 plasmid deposits in 2014 which has grown... -
Podcast: Interview with Addgene's Founders
TypeBlog Post...you’ve ever been curious about what it takes to start your own nonprofit, this is the episode for you....the ground Chapter 3 - Addgene as a leader in standardized tech transfer and the adoption of the UBMTA ...Developing Addgene as a modern e-commerce company and establishing credibility Chapter 5 - Realizing that Addgene... -
Addgene’s Blog Gets a New Look!
TypeBlog Post...you with your experiments, but a new look. We started the blog in 2013 to connect with the scientific...dropdown menu. These pages are a great place to start if you’re new to the topic. Or, try the search bar...articles on the Addgene blog by subscribing to instant alerts below. ... -
Congratulations, Deck The Lab winners!
TypeBlog Post...showcase it all! View this post on Instagram A post shared...able to #DeckTheLab and continue the tradition we started last year: The Christmas Decoration Competition.... 🙌 🥂 🎄 🎉 😷 Remember wear a mask,↔️social distance, 💓show gratitude,☎️ call up an old friend. Remind... -
Interview with Bart Newland, Addgene’s Newest Board Member
TypeBlog Post...Newland is General Counsel at Atalanta, an early-stage biotech company that is developing RNAi therapeutics...transition now to being general counsel of a small start-up. For the last 20 years, I was a patent counsel...different jobs. Being general counsel at a small start-up is very interesting. It’s very varied. There’... -
AAV Packaged on Request is Here!
TypeBlog Post...our new AAV Packaged on Request webpage to get started with your browsing and requesting. If you’re curious...easier to go from selection to viral vector. To start the AAV packaged on request process, simply navigate...placed, we’ll facilitate the necessary MTA, then start production and quality control. Our packaged on... -
Visualizing Your Plasmid Request Data
TypeBlog Post..., after downloading my plasmid request data, I started to explore how I could visualize the aggregated... 2) and the dashboard displays the data in a standardized way. Since Addgene encourages the public sharing...metrics! About the Author Joachim Goedhart is an assistant professor at the Section of Molecular Cytology... -
A Scientist's Guide to Ebola
TypeBlog Post...Dallas was the first to be infected in the United States while caring for a patient, despite wearing protective...Recommended Reading For those looking to delve deeper or stay tuned, there are plenty of online resources available...available. I recommend a few: -The recently established Ebola Genome Portal at the University of California... -
Addgene's Website Update
TypeBlog Post...Starting today, you may notice some changes to our website! Part of sharing science and making access...plasmids from a specific paper. If you’re ready to start a deposit, simply click on “Deposit Materials” and...may find one of your most frequently used buttons stands out better on the page, or that the text is just... -
A Conference By Postdocs For Postdocs: Future of Research
TypeBlog Post... in some form? There is a lot of talk about the state of scientist training in the US and around the world...postdoc-driven biotechnology clubs such as Penn State, Berkeley and Harvard. Students also partner with...their own hands, there are some easy ways to get started. I am happy to work with any group of trainees ... -
Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton
TypeBlog Post...Goals of the Center for Regenerative Meidince - Understanding and developing means to treat disease through...surfactant producing cells that can be used to better understand the biology of certain lung diseases. Chapter...Outro - remember to step back and ask, "Why did I start doing this in the first place?" Many thanks to ... -
Quick, Versatile Plant Transgenesis with GreenGate Plasmids
TypeBlog Post...interest, C-terminal tags, plant terminators and resistance cassettes for selection of transgenic plants.... available at Addgene, also gives the option to stack several expression cassettes onto a single T-DNA...that they cut at arbitrary sequences a defined distance away from the recognition site. In other words... -
Top 10 Open Science Developments of 2013
TypeBlog Post... after all, with each generation of scientists standing on the shoulders of their colleagues and all those...Also in June, the G8 Science Ministers issued a statement after a meeting in London on the global science... as much about open access as it does about the state of peer review. 8) That same issue of Science...