We narrowed to 294 results for: cas9

Studying the biophysics of Cas9 DNA interaction, the Corn lab has developed a means to enhance CRISPR genome editing through homology directed repair.

Cpf1, a newly discovered Cas9 homolog, is smaller than Cas9, can target different sequences, and may more efficiently generate targeted mutations.

Scientists are currently developing disease treatments using CRISPR. Learn about recent developments in the fight against HIV-1 with CRISPR.

Learn about different strategies used to generate plasmids for multiplex gRNA expression and how to use them in various organisms.

A review of alternative PAM and PAM-flexible Cas enzymes.

With it's small crRNAs and RNase activity, Cpf1 can easily be used for multiplex genome editing. Learn more about new multiplex Cpf1 tools here.

Learn about CRISPR terminology for common DNA, proteins, and RNAs involved in this CRISPR 101 blog post.

This blog series showcases some of the new CRISPR tools we have at Addgene each quarter.

If you’re using CRISPR to make a genome edit, how do you know if your experiment was successful? Try using fluorescent CRISPR reporters like SRIRACCHA and GEmCherry2.

Check out how you can use the top lentivirus and AAV from 2016 in your own research.

Nanoblades are a CRISPR delivery tool. These Nanoblades are engineered MLV-like particles loaded with Cas9-gRNA ribonucleoproteins.

Learn more about non-homologous end joining (NHEJ) and how it relates to genome engineering technologies such as CRISPR/Cas9.

Find information on CRISPR mammalian expression systems and delivery methods. Use the table to quickly compare between the methods.

Use these CRISPR base editing reporters to track base editing events in real-time and enrich for base edited cells.

Learn about the latest CRISPR plasmids deposited at Addgene including plasmids for CRISPRi in Burkholderi, DNA and RNA editing, epitope tagging, and Cas9/gRNA ribonucleoprotein delivery.

The purpose of this article is to explain what Unique Persistent Identifiers are, why they’re important, and to announce that Addgene now supports two different formats of plasmid identifiers and how they should be used.

An overview of gRNA modifications available to CRISPR users and some of the advantages of using synthetically modified guides in your experiments.

Learn about CRISPR plasmids at Addgene such as an E. coli genome-wide CRISPRi library, GeneWeld vectors, CasX plasmids, and drug inducible CRISPR/Cas9 activation.

This post discusses the applications, advantages, and caveats to using hybrid guide RNAs, in which DNA bases are substituted for RNA bases.

iTOP is a protein-only transduction method for delivering proteins and RNA into a variety of cell types, with potential applications with CRISPR/Cas9.