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Learn more about the common E. coli strains used in the lab.

The yeast two hybrid (Y2H) assay is useful for probing interactions between proteins. Learn more about the Y2H assay and how you can use it in this post.

With the addition of ABE8, a faster and improved adenine base editor, scientists can now make base edits more efficiently and in more contexts.

Protocol for Ligation Independent Cloning

Read our gamma-retroviral guide to learn about gamma-retroviral components, retroviral production, and common uses.

Baby KJ was treated for his devastating genetic disorder with CRISPR base editing. This post describes the prior advances that made his cure possible.

A dive into troubleshooting and optimization steps you can take to improve your western blot or identify and fix issues within the assay.

The tools of optogenetics can be combined with CRISPR to generate inducible genome edits and gene regulation systems.

REPAIR and RESCUE are two CRISPR tools for RNA editing based on Cas13b.

Learn about the basics of molecular biology, including molecular genetics, plasmids, sequencing, and more.

Get the basics on RNA interference (RNAi), how it compares with CRISPR, and tools for RNAi studies in plants.

Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening.

Addgene's protocol for using the pLKO.1 cloning vector, a backbone used by the RNAi consortium for targeting human and mouse genes.

Learn about bacterial and eukaryotic promoters, including transcription and the transcription complex.

An overview of cytosine and adenine base transition editors, which make precise changes in DNA bases without causing double-strand breaks.

Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening.

Browse neurodegeneration plasmids for research related to Alzheimer's Disease, ALS, Frontotemporal Dementia, Huntington's Disease, Parkinson's Disease, and others.