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We narrowed to 77 results for: codon optimized

Showing: 61 - 77 of 77 results

  2. Tetracycline Inducible Expression

    Type
    Collection
    ...proteins in mammalian cells or other species by codon optimization of their sequence and eliminating interactions...Resnitzky et al., 1994). TRE3G promoter : also optimized into P TRE3GV for viral expression and P TRE3GS...Klaver, B., Berkhout, B., & Das, A. T. (2006). Optimization of the Tet-On system for regulated gene expression...

  3. Luciferase Plasmid Collection

    Type
    Collection
    ...luciferase (Gluc) NanoLuc® (Nluc) The mammalian codon-optimized version of firefly luciferase is often referred...® protein production. Other Sensors and Tools Optimized STARR-seq (Self-Transcribing Active Regulatory...

  6. Plasmids for Stem Cell Research

    Type
    Collection
    ...p53 for single plasmid reprogramming Novel codon-optimized mini-intronic plasmid for efficient, inexpensive... Fibroblasts Cardiomyocytes Lentiviral Mouse Optimization of direct fibroblast reprogramming to cardiomyocytes...transcriptionally enriched signaling pathways to optimize the conversion of fibroblasts into neurons. Elife...

  7. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...Designing shRNA Oligos for pLKO.1 B.1 Determine the optimal 21-mer targets in your gene B.2 Order oligos compatible... F.1 Recommended materials F.2 Determining the optimal puromycin concentration F.3 Protocol for lentiviral...Designing shRNA Oligos for pLKO.1 B.1 Determining the Optimal 21-mer Targets in your Gene Selection of suitable...silencing: Starting at 25nt downstream of the start codon (ATG), search for 21nt sequences that match the ...plasmid to obtain the ratio that gives you the optimal viral production. d. Create a master mix of FuGENE...located in the “Appendix”. F.2. Determining the Optimal Puromycin Concentration Each cell line responds...Addgene strongly recommends that you determine the optimal puromycin concentration for your cell line before...

  8. COVID-19 Resources

    Type
    Collection
    ...protein production, secretion, and codon-optimized versions designed for your expression system...

  9. Molecular Biology Reference

    Type
    Guide
    ...termed a codon. There are 64 codon combinations and only 20 natural amino acids, so multiple codons can encode...multiple codons code for each amino acid. There are 20 amino acids plus a start and stop codon. Below you...ochre) *AUG is the most common start codon. Alternative start codons include CUG in eukaryotes and GUG in...degeneracy. Refer to the amino acid table below for codon-amino acid pairs. Over the past two decades, researchers...also expanded the genetic code by redirecting some codons to encode for synthetic amino acids. Plasmids and...of replication, and an MCS. They are small and optimized to help in the initial cloning of a DNA fragment...field of recombinant DNA technology have been optimized for studying and manipulating genes. For instance...

  10. CRISPR Guide

    Type
    Collection
    ... an optimized gRNA for your application (see: gRNA Design Software ). In addition to an optimized gRNA... frameshift mutations leading to premature stop codons within the open reading frame (ORF) of the targeted...structured motif to enhance pegRNA stability PEmax — optimized PE architecture; increased synergy between PE ...compared to HDR. Prime editing requires more optimization and typically has lower efficiency (< 50%) than.... gRNA design for CRISPR libraries is usually optimized to select for gRNAs with high on-target activity...shifting the ORF and/or creating premature stop codons NHEJ N on- H omologous E nd J oining; A DNA repair...sequence ORF O pen R eading F rame; The translated codons that make up a gene PAM P rotospacer A djacent ...

  11. Promoters

    Type
    Guide
    ...The coding strand is the DNA strand that encodes codons and whose sequence corresponds to the mRNA transcript...promoter region is located closest to the start codon and contains the RNA polymerase binding site, TATA...The most 3' portion (closest to the gene's start codon) of the core promoter is the TSS, which is where...Tetracycline response element promoter; often optimized for mammalian systems Types of RNA Polymerases...

  12. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...synaptic glutamate response. They generated variants optimized for different applications (v82 has higher affinity...indicator (GEVI) called JEDI-2P (Liu et al. 2022) optimized for two-photon imaging. They use a unique and ...multiparameter screen for accelerated development and optimization of soluble genetically encoded fluorescent biosensors...variety of imaging conditions (155 μs and <0.1 °C in optimal conditions). While B-gTEMP itself is a significant...viral prep here: pAAV.hSyn.FLEX.iGluSnFR3.v857.PDGFR.codonopt    Aggarwal A, et al. Glutamate indicators ...

  13. Optogenetics Guide

    Type
    Guide
    ...been created via genetic point mutations, codon optimization, and chimeric fusion of domains from two ...engineered with enhancements such as: Human codon optimization Example: Halo Increased photocurrent amplitude...

  14. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...lab modified the nuclear localization signals and codon usage BE4 to create BE4max and AncBE4max with a ...cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nature Biotechnology...

  15. CRISPR Guide

    Type
    Guide
    ... an optimized gRNA for your application (see: gRNA Design Software ). In addition to an optimized gRNA... frameshift mutations leading to premature stop codons within the open reading frame (ORF) of the targeted...structured motif to enhance pegRNA stability PEmax — optimized PE architecture; increased synergy between PE ...compared to HDR. Prime editing requires more optimization and typically has lower efficiency (< 50%) than.... gRNA design for CRISPR libraries is usually optimized to select for gRNAs with high on-target activity...shifting the ORF and/or creating premature stop codons NHEJ N on- H omologous E nd J oining; A DNA repair...sequence ORF O pen R eading F rame; The translated codons that make up a gene PAM P rotospacer A djacent ...

  16. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...different retroviral vectors using a novel, CoDOn-Optimized gene for chimeric GALV envelope. Viruses , ...

  17. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Gene Type Wildtype Mutant Partial Sequence Codon-Optimized gRNA Other Tags GFP FP other Flag GST His TEV... Pickrell 131792 pHAGE-TBK1-C-FLAG-HA TBK1 STOP codon CMV ALS Alicia Pickrell 131889 pDONR221_SLC1A3 SLC1A3...
Showing: 61 - 77 of 77 results