We narrowed to 294 results for: cas9

Baby KJ was treated for his devastating genetic disorder with CRISPR base editing. This post describes the prior advances that made his cure possible.

Genome-wide CRISPR/Cas9 screens are a high-throughput systematic approach for identifying genes involved in a biological process. Recently the Bassik Lab at Stanford developed a new genome-wide CRISPR knockout screen to analyze unanswered questions about CRISPR screen design by including safe targeting guides and short gRNAs.

The labs of John Doench and David Root have recently created CRISPR libraries to knock out, inactivate, or activate genes for genome-wide screens. These optimized libraries are effective for probing gene function while using fewer sgRNAs.

Cas13a is an RNA cleaving enzyme that can be used to detect specific RNAs. Learn about this and other applications, like nucleic acid detection.

Overview of polycistronic glycine tRNA-gRNA (PTG) multiplexing gRNA cassettes, with comparisons to Pol III and Cys4 gRNA cassettes.

X-CHIME, a series of CRISPR tools developed and deposited by Arlene Sharpe's lab, allows for germline knockouts in immune cells in mice.

Learn about the latest CRISPR plasmids deposited at Addgene such as an E. coli genome-wide CRISPR inhibition library, SECURE base editors, and Nme2Cas9.

Learn how to optimize CRISPR-activated homology directed repair to make targeted edits in mammalian cells.

Designing a CRISPR experiment in bacteira? Check out these tools for genome engineering, CRISPRa, CRISPRi, base editing, and microbiome engineering.

New CRISPR pooled libraries, such as SAM, CRISPRa, and CRISPRi, offer new ways to do genome-wide functional screens.

Find plasmids for CRISPR-Cas and Cascade for large genomic deletions.

This post describes CRISPR-derived base editors to convert cytosine to adenine or guanine, called CGBEs or GBEs.

Learn about TnpB, a small genome editor with a function similar to CRISPR systems!

Check out the latest Hot Plasmids, viral vectors, and antibodies in the Addgene repository!

Learn how to use in vitro screening and other tricks to expedite the CRISPR genome editing process in mice.

Hear from the authors about the generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9.

Learn how you can use HDR and CRISPR to make a knock-in cell line for your research.

The CRISPR babies saga shows us that we need action to move forward in bringing CRISPR technologies from bench to bedside.

Find out how you can use new CRISPR tagging plasmids from the Allen Institute for Cell Science to tag your cellular structure of interest.

Check out all of the plasmids featured in our hot plasmids articles from 2017.