We narrowed to 131 results for: multiplex

1. Typing CRISPR Systems

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   Blog Post

   ...gRNAs under a single promoter, allowing for easy multiplexing. The double-stranded breaks caused by Cas12 ...Jiang, W., Marraffini, L. A., & Zhang, F. (2013). Multiplex genome engineering using CRISPR/CAS systems. Science...

2. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

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   ...such as primer and plasmid design. Examples:  “multiplex design”, “multi-method design”, and “single-method... common utility functions are batch design or multiplex features. However, tools aiding in primer design...

3. OCaMP: A New Calcium Indicator for Neural Imaging

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   ...Modern neuroscience experiments often rely on multiplexing, where multiple fluorescent tools are used simultaneously...

4. Fluorescent Proteins 101: Luciferases

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   ... with incompatible substrates can also allow multiplexing with experiments that supply both substrates...

5. Antibodies 101: Selecting the Right Antibody

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   ...chance that the antibody could meet your needs.    Multiplex experiments Finally, consider whether you will...

6. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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   ...ABE8e. ABE8e is not only fast, it can also be multiplexed: when tasked to correct two point mutations that...

7. Viral Vectors 101: Biosensors

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   ...models. Some of them have even been expanded for multiplexed imaging for advanced monitoring of different ...

8. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

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   ...nt direct repeat) mediating potent knockdown. Multiplexing from the CRISPR array yielded >90% knockdown...

9. Antibodies 101: Fab Fragments

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   ...Engineered fast-dissociating antibody fragments for multiplexed super-resolution microscopy. Cell Rep Methods...

10. What's New in CRISPR - November 2021

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    ... further demonstrated that PRIME-del can be “multiplexed” using pooled paired pegRNAs.  Read the paper...

11. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

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    ...to create high-performance sensors to enable multiplexed imaging of neurotransmitter, calcium, and membrane...

12. Plasmids 101: Modular Cloning

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    ... Cas nuclease. MoClo can be used to assemble multiplexed gRNA systems as well as additional components...

13. CRISPRainbow and Genome Visualization

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    ...publication at Addgene. 3. Ma, Hanhui, et al. “Multiplexed labeling of genomic loci with dCas9 and engineered...

14. Popular Retroviral Vectors and Their Uses in Scientific Research

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    ...combined with high-throughput sequencing to perform multiplexed analysis of cells pre-treated with a compound...

15. CRISPR 101: Cas9 vs. The Other Cas(s)

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    ...used in lieu of siRNA or shRNA for efficient, multiplexable RNA knockdown. Cas13 has also stirred up interest...

16. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

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    ...recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. eLife 8: . https://doi.org...

17. Hot Plasmids - October 2020

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    ... the plasmids at Addgene The Cas Hybrid for Multiplexed Editing and screening Applications (CHyMErA) ...

18. 6 Tips for Grant Writing

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    ...great information on ELISA troubleshooting and multiplex assays.   Additional Resources on the Addgene ...

19. 15 Hot Plasmids from 2017

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    ...segment on regulating telomere length with TZAP Multiplexible plant genome engineering toolkit The Voytas ... well as Cys4, tRNA, and ribozyme systems for multiplex gRNA expression. Expression of toolkit components...

20. Antibody Guide

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    ... controls. Multiplex Assays Flow cytometry, IF, IHC, and ICC are typically run as multiplex assays. Both...Methods Visualization Methods Cell Sorting Methods Multiplex Assays Selection Validation Affinity reagents ...targets, and is described in more detail in the Multiplex Assays section below. The following table describes...distinguish between the primary antibodies in multiplex assays. This is done by using different isotypes...means the majority of IF assays are considered multiplex assays, even if they only have one protein of ...additional markers to distinguish cell types. Multiplex assays can range from two antibodies to upwards...currently are in the mid-forties. When designing a multiplex assay, selection of the fluorophores used is important...