We narrowed to 17 results for: multiplex

...Return to top Multiplex gRNA Kits Many CRISPR plasmid kits at Addgene contain multiplex-compatible gRNA...gRNA Expression Vectors gRNA and Cas gRNA Only Multiplex Resources CRISPR requires you to express both ...Selectable Marker PI Publication Return to top Multiplex gRNA Plasmids ID Plasmid Vector Type Promoter ...gRNA plasmids, including: Multiplex CRISPR/Cas9 Assembly Systems Multiple Lentiviral Expression System ...

... Depositor Collections MORF Feng Zhang - Multiplexed Overexpression of Regulatory Factors (MORF) Collection...

... controls. Multiplex Assays Flow cytometry, IF, IHC, and ICC are typically run as multiplex assays. Both...Methods Visualization Methods Cell Sorting Methods Multiplex Assays Selection Validation Affinity reagents ...targets, and is described in more detail in the Multiplex Assays section below. The following table describes...distinguish between the primary antibodies in multiplex assays. This is done by using different isotypes...means the majority of IF assays are considered multiplex assays, even if they only have one protein of ...additional markers to distinguish cell types. Multiplex assays can range from two antibodies to upwards...currently are in the mid-forties. When designing a multiplex assay, selection of the fluorophores used is important...

...genome engineering, offering flexibility and easy multiplexing/scaling. Prokaryotes have long utilized CRISPR...the tool’s specificity, orthogonality, and multiplexibility in various species, as well as the development...Cpf1's small size also makes it suitable for multiplexing and use in AAV. Scientists have also developed... Wu X, Jiang W, Marraffini LA, Zhang F. 2013. Multiplex genome engineering using CRISPR/Cas systems. Science...Huisman M, Zhang S, Grunwald D, Pederson T. 2016. Multiplexed labeling of genomic loci with dCas9 and engineered..., Severinov K, van der Oost J, Zhang F. 2017. Multiplex gene editing by CRISPR-Cpf1 using a single crRNA...

...detail later. Read more about Cas9 multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9...Figure 2: Overview of the NHEJ repair mechanism. Multiplex Genome Engineering Many CRISPR experiments involve...be expressed in the same cell. This is called multiplexing , and increases the likelihood that any cell...all desired genomic edits carried out by Cas9. Multiplexing applications include editing multiple genes ...with two gRNA sites on the same chromosome. Most multiplex systems enable researchers to target anywhere ...achieved targeting in the double digits. These multiplex gRNA vectors can be used to knock out, activate...enzymes, such as Cas12a or Cas13 , can improve multiplexing efficiency and are discussed in more detail ...

...functional variants of Cas9. A CRISPR/Cas9 toolkit for multiplex genome editing in variety of plant species (e....mutation. Combined fluorescent seed selection and multiplex CRISPR/Cas9 assembly for fast generation of multiple...CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants. Plant...

...FreeGenes Project Open Enzyme Collection Screening Multiplexed Overexpression of Regulatory Factors (MORF) Collection...

...mitochondria Mitycam, or ER targeted GCaMPer Multiplexed Optical Sensors in Arrayed Islands of Cells for... exceptional brightness, photostability, and multiplexing capabilities. bioRxiv. 2025 Jan 11:2024.12.16.628673...Single-fluorophore biosensors for sensitive and multiplexed detection of signalling activities. Nat Cell ...GFP-based fluorescent reporter cAMPr Spatial Multiplexing of Fluorescent Reporters for Imaging Signaling...Cl-) ClopHensorN chloride sensor (cytosolic) Multiplexed Optical Sensors in Arrayed Islands of Cells for...mitochondrial sensor for H 2 O 2 oxidation (roGFP2-Orp1) Multiplexed Optical Sensors in Arrayed Islands of Cells for...RdLight1 An expanded palette of dopamine sensors for multiplex imaging in vivo. Nat Methods. 2020 Nov;17(11):...

...intersectional expression plasmids that combine multiplexed recombinases, including Cre, Flp, and VCre to...Intersectional expression approaches combine multiplexed recombinases, including Cre, Flp, and VCre to...definitions using recombinases. A) Cre and Flp are multiplexed to enable intersectional viral targeting of populations...

...), 537–541. DOI: 10.1021/sb400021j Figure 1: Multiplexing CRISPR knockdown to modulate expression of two...

...Resources Addgene Kits MoClo-YTK Yeast Gateway Kit Multiplex Yeast Toolkit (MYT) Markerless Yeast Localization...

...Propionate Induction Lim Lab RNA Feedback Lu Lab Multiplexed/Programmable Gene Networks Mascher Lab Bacillus...

...generates unique combinations of protein bits. B) Multiplex imaging of barcodes with expansion microscopy ...

... in genome editing of bacteria. CRISPathBrick Multiplex Plasmid : CRISPR expression in E. coli - Koffas...

...interest into the genome at the defined FRT site. Multiplexing: Orthogonal recombinase systems can be combined...

...20bp). Return to top References SpCas9 plasmids Multiplex Genome Engineering using CRISPR/Cas Systems. Cong...

...subclass from the conventional mAb to facilitate multiplex labeling with subclass-specific secondary antibodies...recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. eLife . 8:e43322. PMID...