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Plant-75.png Plant Plasmids and Resources


Background

Addgene’s repository contains many plasmid tools that were specifically designed for Plant Biology. These resources include tools for research using plant systems such as Arabidopsis, wheat, maize, rice, tomato, and a variety of other species. In addition to individual plasmids, Addgene carries several useful toolkits and plasmid collections for Plant Biology. Check out the Plant CRISPR and Plant SynBio collections!

The tables below highlight plasmids spanning a wide variety of molecular biology technologies: from genome engineering and gene regulation to fluorescent proteins. Addgene’s collection includes plasmids containing promoters that drive general and species-specific gene expression (see the column ‘promoter’ in the tables for species-specific tools).

Support open science in Plant Biology - deposit plant plasmids with Addgene!

CRISPR

Please refer to Addgene's CRISPR Guide for a general introduction to CRISPR technology or the Plant CRISPR resources for a full selection of plasmids expressing different functional variants of Cas9 and empty gRNA backbones.

A few examples of useful plant CRISPR resources include:

  • A Multipurpose Toolkit that enables targeted, specific modification of monocot and dicot genomes using a variety of genome engineering approaches including TALENs and CRISPR. Validated in tomato (Solanum lycopersicum), tobacco (Nicotiana tabacum), Medicago truncatula, wheat (Triticum aestivum), and barley (Hordeum vulgare) - Daniel Voytas.
  • Cas9 and gRNA backbone for use in Nicotiana benthamiana - Sophien Kamoun.
  • Plant codon optomized Cas9 for use in Arabidopsis and Nicotiana benthamiana - Jen Sheen.
  • pKIR Vectors for highly efficient CRISPR/Cas9-mediated gene knockout in Arabidopsis thaliana - Tetsuya Higashiyama.
  • A CRISPR/Cas9 toolkit for use in a variety of plant species (e.g. maize and Arabidopsis) and vectors for easy assembly of one or more gRNA expression cassettes - Qi-Jun Chen.
  • Cas9 and gRNA backbone for use in rice - Yinong Yang. Includes a protocol for Targeted Gene Mutation in Rice.
  • pDGE Dicot Genome Editing Kit for Golden Gate cloning-based assembly of constructs for CRISPR genome editing in dicotyledonous plants - Johannes Stuttmann.
  • Plasmids for CRISPR base editing in:

Cloning

The following collections are useful for creating plant expression vectors:

ORFeome Collections

The following collections are useful for studying gene expression in plants:

RNAi

The following collections are useful for gene silencing in plants:

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Empty Backbones

The table below highlights plasmids that can be used as empty backbones for your gene of interest for expression in plants. You can search the table for features you are looking for, such as the promoter or cloning method of choice.

ID Plasmid Purpose Promoter Cloning Method PI
33297pUNos_C1maize ubiquitin (Ubi-1) None Katagiri
36184pFZ19XVE Unknown Voytas
44987pZHY05135S Gateway Cloning Voytas
61704pGTQL1211YNGateway compatible BiFC vector (pEarleyGate201-YN)35S Gateway Cloning Cui
61705pGTQL1221YCGateway compatible BiFC vector (pEarleyGate202-YC)35S Gateway Cloning Cui
67583pSIM24 KanRPlant Gene Expression VectorM24 Restriction Enzyme Maiti
68580pMpGWB126Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants Gateway Cloning Kohchi
68581pMpGWB127Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsMpEF1alpha promoter Gateway Cloning Kohchi
68654pMpGWB326Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants Gateway Cloning Kohchi
68691pMpGWB426Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants Gateway Cloning Kohchi
71745pTA7001-DESTGATEWAY compatible derivative of plant binary vector pTA7001 from Nam Chua's laboratory at The Rockefeller University, Dexamethasone inducibleGAL4 Restriction Enzyme Innes
74795pGWB401Gateway cloning compatible binary vector for simple cloning of gene. Gateway Cloning Nakagawa
74796pGWB402Gateway cloning compatible binary vector for expression of gene by CaMV35S promoter.CaMV35S Gateway Cloning Nakagawa
74797pGWB402-omegaGateway cloning compatible binary vector for expression of gene by 2xCaMV35S enhancer and omega sequence.2xCaMV35S enhancer and omega Gateway Cloning Nakagawa
74808pGWB414Gateway cloning compatible binary vector for C-terminal fusion with 3xHA (CaMV35S promoter).CaMV35S Gateway Cloning Nakagawa
74809pGWB415Gateway cloning compatible binary vector for N-terminal fusion with 3xHA (CaMV35S promoter).CaMV35S Gateway Cloning Nakagawa
74843pGWB501Gateway cloning compatible binary vector for simple cloning of gene. Gateway Cloning Nakagawa
74844pGWB502Gateway cloning compatible binary vector for expression of gene by CaMV35S promoter.CaMV35S Gateway Cloning Nakagawa
74845pGWB502-omegaGateway cloning compatible binary vector for expression of gene by 2xCaMV35S enhancer and omega sequence.2xCaMV35S enhancer and omega Gateway Cloning Nakagawa
74873pGWB535Gateway cloning compatible binary vector for C-terminal fusion with LUC (no promoter). Gateway Cloning Nakagawa
74883pGWB553Gateway cloning compatible binary vector for C-terminal fusion with mRFP (no promoter). Gateway Cloning Nakagawa
80082pJL-TRBOAn improved agroinfection-compatible Tobacco mosaic virus (TMV)-based transient expression vector that lacks the TMV CP gene coding sequence. None Lindbo
86198pYPQ202Multisite Gateway T-DNA entry plasmid (attR1 & attR2); AtUBQ10 promoter and Hygromiycin resistance markerArabidopsis ubiquitin 10 (AtUbi10) Restriction Enzyme Qi
86207pYPQ203 (pMDC32-Ubi1)Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and Hygromiycin resistance markerMaize ubiquitin 1 (ZmUbi1) Restriction Enzyme Qi
91110pTRANS_211Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: BeYDV Gibson Cloning Voytas
91112pTRANS_213Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: WDV Gibson Cloning Voytas
91115pTRANS_221Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: BeYDV Gibson Cloning Voytas
91116pTRANS_222Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: ToLCV Gibson Cloning Voytas
91117pTRANS_223Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: WDV Gibson Cloning Voytas
91120pTRANS_231Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: BeYDV Gibson Cloning Voytas
91125pTRANS_253Transformation, Type: T-DNA, Plant Selection: PvUbi2:hpt II, Viral Replication: WDV Gibson Cloning Voytas
91127pTRANS_263Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: WDV Gibson Cloning Voytas
98558pJL 89Useful for making infectious clones of RNA virus cDNAs; Has 35S promoter and HDV Ribozyme sequences in T-DNA region of Agrobacterium vector.Enh 35S None Lindbo

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Fluorescent Proteins

The table below highlights plasmids that feature fluorescent or luminescent proteins for expression in plants. You can search the table for features you are looking for, such as the promoter or fluorescent protein of choice.

ID Plasmid Purpose Promoter PI
50304pICSL30003 Patron
50316pICSL50004 Patron
61628pGreenIIM DR5v2-ntdTomato/DR5-n3GFPExpresses nuclear tdTomato from auxin-dependent DR5v2 (TGTCGG) promoter and nuclear 3xGFP from the DR5 (TGTCTC) promoterAuxin-dependent DR5v2 promoter containing 9 reverse TGTCGG sites and -46 35S promoter, Auxin-dependent DR5 promoter containing 9 reverse TGTCTC sites and -46 35S promoter Weijers
61629pGreenIIM RPS5A-mDII-ntdTomato/RPS5A-DII-n3VenusR2D2: Expresses a fusion of auxin-sensitive DII to nuclear 3xVenus and auxin-insensitive mDII to nuclear tdTomato, both from the Arabidopsis RPS5A promoterRPS5A, RPS5A Weijers
64257pGH00.0126Binary T-DNA vector expressing EGFP and neomycin phosphotransferase II (NPTII); for Agrobacterium tumefaciens-mediated transformationE12-omega, E12-omega Guiltinan
64396pHMWGFPRHigh molecular weight glutenin gene promoter driving GFP expressionHigh molecular weight glutenin gene promoter Vickers
64401pGFPGUSPlusbinary plant vector expressing GFP and GUS, both driven by a 35S promoter35S, 35S Vickers
68215pEGB 35S:Renilla:Tnos (GB0109)Transcriptional unit for Renilla Protein plant expression driven by the 35S promoter35S Orzaez
68216pEGB 35S:Luciferase:Tnos (GB0110)Transcriptional unit for Firefly Luciferase Protein plant expression driven by the 35S promoter35S Orzaez
74798pGWB404Gateway cloning compatible binary vector for C-terminal fusion with sGFP (no promoter). Nakagawa
74799pGWB405Gateway cloning compatible binary vector for C-terminal fusion with sGFP (CaMV35S promoter).CaMV35S Nakagawa
74827pGWB441Gateway cloning compatible binary vector for C-terminal fusion with eYFP (CaMV35S promoter).CaMV35S Nakagawa
74828pGWB442Gateway cloning compatible binary vector for N-terminal fusion with eYFP (CaMV35S promoter).CaMV35S Nakagawa
74830pGWB444Gateway cloning compatible binary vector for C-terminal fusion with eCFP (CaMV35S promoter).CaMV35S Nakagawa
74833pGWB451Gateway cloning compatible binary vector for C-terminal fusion with G3GFP (CaMV35S promoter).CaMV35S Nakagawa
74835pGWB453Gateway cloning compatible binary vector for C-terminal fusion with mRFP (no promoter). Nakagawa
74836pGWB454Gateway cloning compatible binary vector for C-terminal fusion with mRFP (CaMV35S promoter).CaMV35S Nakagawa
74837pGWB455Gateway cloning compatible binary vector for N-terminal fusion with mRFP (CaMV35S promoter).CaMV35S Nakagawa
74846pGWB504Gateway cloning compatible binary vector for C-terminal fusion with sGFP (no promoter). Nakagawa
74847pGWB505Gateway cloning compatible binary vector for C-terminal fusion with sGFP (CaMV35S promoter).CaMV35S Nakagawa
74848pGWB506Gateway cloning compatible binary vector for N-terminal fusion with sGFP (CaMV35S promoter).CaMV35S Nakagawa
74874pGWB540Gateway cloning compatible binary vector for C-terminal fusion with eYFP (no promoter). Nakagawa
74875pGWB541Gateway cloning compatible binary vector for C-terminal fusion with eYFP (CaMV35S promoter).CaMV35S Nakagawa
74884pGWB554Gateway cloning compatible binary vector for C-terminal fusion with mRFP (CaMV35S promoter).CaMV35S Nakagawa
74885pGWB555Gateway cloning compatible binary vector for N-terminal fusion with mRFP (CaMV35S promoter).CaMV35S Nakagawa
74992pBGN155GFP(S65T)-based BiFC vector for transient expression of sGN (1-155) in plantsCaMV35S Wada
75412pEGB SF-35S:Renilla:TNOS-35S:P19:TNOS (GB0160)Module for the expression of the Renilla Luciferase with the silencing suppressor P1935S Orzaez
7918335S-DsREDmonomer-NosTTransient expression vector for DsRED monomerCaMV35S Wada
80083pJL-TRBO-GAn improved agroinfection-compatible Tobacco mosaic virus (TMV)-based transient expression vector that lacks the TMV CP gene coding sequence and expresses GFP. Lindbo
8012735S-eGFP-nosTTransient expression vector for eGFP in plantsCaMV35S Kodama
8012935S-sfGFP-nosTTransient expression vector for sfGFP in plantsCaMV35S Kodama
89487YFN43gateway-based Bimolecular Fluorescence Complementation (BiFC) binary vector containing N terminal fragment of YFP2x35S Ferrando
89488YFC43gateway-based Bimolecular Fluorescence Complementation (BiFC) binary vector containing C terminal fragment of YFP2x35S Ferrando
97423pEffTransient expression of recombinant proteins in plants. The plasmid contains gfp, which is replaced by the gene of interest. Ravin
132565pN_35S/mEGFPmEGFP expression regulated by the CaMV35S promoter, co-expressed nourseothricin acetyl transferase selectable marker (nat)Cauliflower mosaic virus 35S Pribil
132566pN_35S/mApplemApple expression regulated by the CaMV35S promoter, co-expressed nourseothricin acetyl transferase selectable marker (nat)Cauliflower mosaic virus 35S Pribil

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Other Resources

Addgene Blog Posts

External Resources

  • Arabidopsis Biological Resource Center (ABRC) - The ABRC collects, preserves, reproduces, and distributes diverse seed and other stocks of Arabidopsis thaliana and related species.
  • The Arabidopsis Information Resource (TAIR) - TAIR includes a database of genetic and molecular biology data for Arabidopsis thaliana research.
  • BAR The Bio-Analytic Resource for Plant Biology - Tools for gene and protein expression and mapping of gene expression in plants.
  • Figshare - Plant Illustrations - Collection of illustrations and pictures of plants, roots, shoots, and inflorescences - a growing resource for the plant science community.
  • Grassius - a public web resource composed by a collection of databases, computational and experimental resources that relate to the control of gene expression in the grasses.
  • OpenPlant Synthetic Biology Research Centre - The initiative promotes interdisciplinary exchange, open technologies, and responsible innovation for improvement of sustainable agriculture and conservation.
  • Medicinal Plant Genomics Resource - Transcriptomic and metabolomic resources for 14 key medicinal plants for the advancement of drug production and development.
  • The TapRoot - Podcast - The Taproot is a podcast to understand how scientific publications are created. Papers from the plant biology literature are discussed with one of the authors. The podcast is produced by Plantae.
  • TraVa - Database of gene expression profiles in Arabidopsis thaliana based on RNA-seq analysis.

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Do you have suggestions for other plasmids that should be added to this list?

Fill out our Suggest a Plasmid form or e-mail [email protected] to help us improve this resource!