We narrowed to 131 results for: multiplex
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TypeBlog Post... for gene activation experiments. In cases of multiplex gene regulation (activating multiple genes at ...
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PCR Applications for Copy Number Variation Assays
TypeBlog Post...different markers, it can also be used to run multiplex assays, allowing the reference sequence and the... -
Hot Plasmids February 2024
TypeBlog Post...achieved with a 30-minute incubation, even with multiplexed guides. Figure 1: A) Cell-penetrating ... -
Pooled Library Amplifications
TypeBlog Post...Libraries (PDF, 401 KB) Screening Zhang Lab - Multiplexed Overexpression of Regulatory Factors (MORF) Library... -
CRISPR Guide
TypeGuide...detail later. Read more about Cas9 multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9...experimentally. Browse Plasmids: Double-Strand Break (Cut) Multiplex Genome Engineering Many CRISPR experiments involve...be expressed in the same cell. This is called multiplexing , and increases the likelihood that any cell...all desired genomic edits carried out by Cas9. Multiplexing applications include editing multiple genes ...with two gRNA sites on the same chromosome. Most multiplex systems enable researchers to target anywhere ...achieved targeting in the double digits. These multiplex gRNA vectors can be used to knock out, activate...enzymes, such as Cas12a or Cas13 , can improve multiplexing efficiency and are discussed in more detail ... -
Cre-Lox and Other Site-Specific Recombinases
TypeCollection...interest into the genome at the defined FRT site. Multiplexing: Orthogonal recombinase systems can be combined... -
New Tools Enable CRISPRa for Neuroscience Applications
TypeBlog Post... then packaged into AAV for in vivo delivery. Multiplex gene targeting is also possible and is only constrained... -
CRISPR 101: RNA Editing with Cas13
TypeBlog Post...curious if REPAIR and RESCUE can function in a multiplexed manner to edit multiple RNAs. It’s clear that... -
Getting to Know Expansion Microscopy
TypeBlog Post...Introduction to Immunofluorescence Antibodies 101: Multiplex Immunofluorescence Antibodies 101: Affinity Reagents... -
Hot Plasmids - November 2023
TypeBlog Post...recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. eLife, 8, e43322. doi... -
Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog Post... Wu X, Jiang W, Marraffini LA, Zhang F (2013) Multiplex Genome Engineering Using CRISPR/Cas Systems. Science... -
CRISPR-based Genome Editing Systems For Editing in E. coli
TypeBlog Post...mainly on the development and application of a multiplex genome editing system based on CRISPR-associated... -
Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design
TypeBlog Post...Resources Watch Dr. Michael Bassik’s lecture on “Multiplexing with CRISPR Screens” ... -
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells
TypeBlog Post... systems. References 1. Cong, Le, et al. "Multiplex genome engineering using CRISPR/Cas systems." ... -
Modular Cloning Guide
TypeGuide...multi-gene constructs for S. cerevisiae expression. Multiplex Yeast Toolkit (MYT) Yeast Expression, CRISPR Tom...cerevisiae , enabling larger multi-gene constructs, multiplexed CRISPR editing, and more. Fungal Toolkit for ...expression vectors for proximity labeling experiments. Multiplex CRISPR/Cas9 Assembly Systems Genome Engineering... -
Hot Biosensors 2022: Year-End Roundup
TypeBlog Post...with different ITAM/SH2 reporter pairs allow multiplexed measurement of different RTKs. Although this ... -
The Advances Behind The World's First Personalized CRISPR Treatment
TypeBlog Post...Jiang, W., Marraffini, L. A., & Zhang, F. (2013). Multiplex genome engineering using CRISPR/Cas systems. Science... -
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons
TypeBlog Post...13):2835-46. doi: 10.1242/dev.094631. PubMed. Multiplex cell and lineage tracking with combinatorial labels... -
Hot Plasmids: Summer 2024
TypeBlog Post...be packaged in AAV vectors, potentially with multiplexed targets. Figure 4: CHARM uses a fusion... -
Generating Mouse Models Using CRISPR/Cas9
TypeBlog Post... Wu X, Jiang W, Marraffini LA, Zhang F (2013) Multiplex Genome Engineering Using CRISPR/Cas Systems. Science...