We narrowed to 138 results for: multiplex

...great information on ELISA troubleshooting and multiplex assays.   Additional Resources on the Addgene ...

... controls. Multiplex Assays Flow cytometry, IF, IHC, and ICC are typically run as multiplex assays. Both...Methods Visualization Methods Cell Sorting Methods Multiplex Assays Selection Validation Affinity reagents ...targets, and is described in more detail in the Multiplex Assays section below. The following table describes...distinguish between the primary antibodies in multiplex assays. This is done by using different isotypes...means the majority of IF assays are considered multiplex assays, even if they only have one protein of ...additional markers to distinguish cell types. Multiplex assays can range from two antibodies to upwards...currently are in the mid-forties. When designing a multiplex assay, selection of the fluorophores used is important...

... controls. Multiplex Assays Flow cytometry, IF, IHC, and ICC are typically run as multiplex assays. Both...Methods Visualization Methods Cell Sorting Methods Multiplex Assays Selection Validation Affinity reagents ...targets, and is described in more detail in the Multiplex Assays section below. The following table describes...distinguish between the primary antibodies in multiplex assays. This is done by using different isotypes...means the majority of IF assays are considered multiplex assays, even if they only have one protein of ...additional markers to distinguish cell types. Multiplex assays can range from two antibodies to upwards...currently are in the mid-forties. When designing a multiplex assay, selection of the fluorophores used is important...

...segment on regulating telomere length with TZAP Multiplexible plant genome engineering toolkit The Voytas ... well as Cys4, tRNA, and ribozyme systems for multiplex gRNA expression. Expression of toolkit components...

...detail later. Read more about Cas9 multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9...Figure 2: Overview of the NHEJ repair mechanism. Multiplex Genome Engineering Many CRISPR experiments involve...be expressed in the same cell. This is called multiplexing , and increases the likelihood that any cell...all desired genomic edits carried out by Cas9. Multiplexing applications include editing multiple genes ...with two gRNA sites on the same chromosome. Most multiplex systems enable researchers to target anywhere ...achieved targeting in the double digits. These multiplex gRNA vectors can be used to knock out, activate...enzymes, such as Cas12a or Cas13 , can improve multiplexing efficiency and are discussed in more detail ...

...detail later. Read more about Cas9 multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9...Figure 2: Overview of the NHEJ repair mechanism. Multiplex Genome Engineering Many CRISPR experiments involve...be expressed in the same cell. This is called multiplexing , and increases the likelihood that any cell...all desired genomic edits carried out by Cas9. Multiplexing applications include editing multiple genes ...with two gRNA sites on the same chromosome. Most multiplex systems enable researchers to target anywhere ...achieved targeting in the double digits. These multiplex gRNA vectors can be used to knock out, activate...enzymes, such as Cas12a or Cas13 , can improve multiplexing efficiency and are discussed in more detail ...

... for gene activation experiments. In cases of multiplex gene regulation (activating multiple genes at ...

...different markers, it can also be used to run multiplex assays, allowing the reference sequence and the...

...Libraries (PDF, 401 KB) Screening Zhang Lab - Multiplexed Overexpression of Regulatory Factors (MORF) Library...

...achieved with a 30-minute incubation, even with multiplexed guides. Figure 1: A) Cell-penetrating ...

...interest into the genome at the defined FRT site. Multiplexing: Orthogonal recombinase systems can be combined...

...Introduction to Immunofluorescence  Antibodies 101: Multiplex Immunofluorescence Antibodies 101: Affinity Reagents...

...curious if REPAIR and RESCUE can function in a multiplexed manner to edit multiple RNAs. It’s clear that...

... then packaged into AAV for in vivo delivery. Multiplex gene targeting is also possible and is only constrained...

...recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. eLife, 8, e43322. doi...

...mainly on the development and application of a multiplex genome editing system based on CRISPR-associated...

...Resources Watch Dr. Michael Bassik’s lecture on “Multiplexing with CRISPR Screens”    ...

... Wu X, Jiang W, Marraffini LA, Zhang F (2013) Multiplex Genome Engineering Using CRISPR/Cas Systems. Science...

... systems.     References 1. Cong, Le, et al. "Multiplex genome engineering using CRISPR/Cas systems." ...

...multi-gene constructs for S. cerevisiae expression. Multiplex Yeast Toolkit (MYT) Yeast Expression, CRISPR Tom...cerevisiae , enabling larger multi-gene constructs, multiplexed CRISPR editing, and more. Fungal Toolkit for ...expression vectors for proximity labeling experiments. Multiplex CRISPR/Cas9 Assembly Systems Genome Engineering...