...controls help you rule out errors like uneven pipetting or irregular membrane transfer. Figure ...important for semi-quantitative western blots. No pipetter is perfect, and proteins may not transfer precisely...

...residue at the N terminus with a small peptide (LPETG). In EXCELL, the mgSrtA variant can be displayed...interacting cell surface proteins with a biotin tagged LPETG peptide. Biotin tagged proteins can then be purified...

...your primers should be free of palindromic and repetitive sequences, but if present, a minor extension ...template should be isolated from a methylation competent bacterial strain (e.g. DH5α which is dam+). Methylation...

...equipment, categorize all of your minor tools, like pipets, or mini-centrifuges, etc. Be sure to capture as...phones.  As for reagents and consumables (such as pipet tips, glassware, etc), get a simple list from your...

...with fluorescent derivatives of its substrate trimpethoprim (TMP-tag) (Miller et al., 2005). Although this...Benzylguanine (BG) Many Easy eDHFR 159 Trimpethoprim  (TMP-tag) Few Easy HaloTag 295 ...

...experiences! These applications can be highly competitive, so asking for help from mentors and other students... Imposter: The Effect of Perceived Classroom Competition on the Daily Psychological Experiences of First-Generation...

...which subpopulation of DA neurons could activate appetitive rather than aversive stimuli. In a recent paper... 756. PubMed PMID: 6493336. Harz, Hartmann, and Peter Hegemann. "Rhodopsin-regulated calcium currents ...

...to contact me at [email protected]. Keep pipetting and happy genome editing! Pooran Dewari is a ... them to study brain tumour biology. When not pipetting, he can be seen daydreaming about sunny days in...

...Neutrophils travel to the sites of infection or damage (Peters et al. 2008, Chtanova et al. 2008, Kamenyeva et... immune system and its dynamics. References 1. Peters, Nathan C., et al. "In vivo imaging reveals an ...

...hypotheses that they are involved in plasmid competition or can hijack machinery from other CRISPR systems...CRISPR–Cas systems are highly diverse and involved in competition between plasmids. Nucleic Acids Research, 48(...

...Unnatural amino acid incorporation in mammalian cells Peter Schultz and colleagues at the Scripps Research Institute...Giannaks et al 2003, Bert et al 2000). In 2000, Peter Cockerill and his lab developed an improved luciferase...available, as well as 5S RNA F30-2xdBroccoli fusion and pET28c-F30-Broccoli controls. Don’t just eat more broccoli...

...activity than BE3; editing window ~1-2 nt  87438  pET42b-HF-BE3  HF-Cas9 (NGG)  Very low off-target activity...et al. Cell Systems. 2016. PubMed PMID: 28189581 Peters J, et al. Cell. 2016. PubMed PMID: 27238023 Listen...

...endothelin 1 ET1, HDLCQ7 EDN2 endothelin 2 ET2, PPET2 EDN3 endothelin 3 ET3, MGC15067, MGC61498 EDNRA ...

...master fundamental technical lab skills, like pipetting! We’ll release a new tip every two weeks during...

...submission.    Our Cultured Warrior (made by Nate using pipette boxes, wire, cable ties) received a festive makeover...

...research on CRISPR and what it's like to work in a competitive field. Chapter 2: Wei Leong Chew's swift transition...

... world. Cap your Eppendorf tubes, hang up your pipettes, and seal up those plates – it’s time to turn ...

... In the future, you’ll be able to access these snippets here or from their respective articles on the ...

...organization we thrive on 3 things: fun, teamwork, and competition. And our Halloween celebration this Friday will...

...bacterial transformation of chemically and electrocompetent cells is minimal (picogram range). It can ...