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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 304 results for: pET

Showing: 121 - 140 of 304 results

  4. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...levels of assertiveness and cooperation: avoiding, competing, accommodating, collaborating, and compromising...Though there may be a time and place for avoiding, competing, or accommodating, if you find yourself gravitating...

  5. March for Science

    Type
    Blog Post
    ...References March for Science Website Locke, John, and Peter Laslett. Locke: Two treatises of government student... 15-008: Perspectives on Broader Impacts. The Pipettepen: The March for Science Raises Concerns Over Politicization...

  6. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...-mimetic sequences that imitate this motif and compete with both small molecule integrin inhibitors and...their targeted integrin subtype, even when in competition with other similar integrins, as reported by ...

  8. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...your primers should be free of palindromic and repetitive sequences, but if present, a minor extension ...template should be isolated from a methylation competent bacterial strain (e.g. DH5α which is dam+). Methylation...

  9. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...equipment, categorize all of your minor tools, like pipets, or mini-centrifuges, etc. Be sure to capture as...phones.  As for reagents and consumables (such as pipet tips, glassware, etc), get a simple list from your...

  11. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...with fluorescent derivatives of its substrate trimpethoprim (TMP-tag) (Miller et al., 2005). Although this...Benzylguanine (BG) Many Easy eDHFR 159 Trimpethoprim  (TMP-tag) Few Easy HaloTag 295 ...

  12. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...experiences! These applications can be highly competitive, so asking for help from mentors and other students... Imposter: The Effect of Perceived Classroom Competition on the Daily Psychological Experiences of First-Generation...

  15. Typing CRISPR Systems

    Type
    Blog Post
    ...hypotheses that they are involved in plasmid competition or can hijack machinery from other CRISPR systems...CRISPR–Cas systems are highly diverse and involved in competition between plasmids. Nucleic Acids Research, 48(...

  16. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...Unnatural amino acid incorporation in mammalian cells Peter Schultz and colleagues at the Scripps Research Institute...Giannaks et al 2003, Bert et al 2000). In 2000, Peter Cockerill and his lab developed an improved luciferase...available, as well as 5S RNA F30-2xdBroccoli fusion and pET28c-F30-Broccoli controls. Don’t just eat more broccoli...

  17. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...activity than BE3; editing window ~1-2 nt  87438  pET42b-HF-BE3  HF-Cas9 (NGG)  Very low off-target activity...et al. Cell Systems. 2016. PubMed PMID: 28189581 Peters J, et al. Cell. 2016. PubMed PMID: 27238023 Listen...

  20. 2021 Deck the Lab Winners!

    Type
    Blog Post
    ...submission.    Our Cultured Warrior (made by Nate using pipette boxes, wire, cable ties) received a festive makeover...
Showing: 121 - 140 of 304 results