We narrowed to 161 results for: grna

Practical advice for using CRISPRs: designing your gRNA and introducing it into cells.

Sept. 24, 2020, 1:15 p.m.

Learn about different strategies used to generate plasmids for multiplex gRNA expression and how to use them in various organisms.

Sept. 10, 2020, 11:45 a.m.

Researchers at the Wyss Institute for Biologically Inspired Engineering explain how you can use truncated gRNAs to regulate gene expression.

Jan. 10, 2017, 3:37 p.m.

Overview of polycistronic glycine tRNA-gRNA (PTG) multiplexing gRNA cassettes, with comparisons to Pol III and Cys4 gRNA cassettes.

June 2, 2015, 6:06 p.m.

An overview of gRNA modifications available to CRISPR users and some of the advantages of using synthetically modified guides in your experiments.

Oct. 20, 2022, 1:15 p.m.

Learn how you can use Green Listed to design gRNAs for custom CRISPR screens.

July 11, 2017, 2:30 p.m.

Learn how to verify indels, deletions, and homology directed repair (HDR) events in your CRISPR modified cell lines.

Nov. 3, 2022, 12:15 p.m.

Use CRISPRainbow to label multiple genome sites with fluorescent proteins of various colors.

Feb. 28, 2017, 3:30 p.m.

New to using CRISPR pooled libraries for your screen? Here, we cover how to choose a CRISPR library, and the steps and tips for a successful CRISPR screen.

Aug. 18, 2015, 2:30 p.m.

Learn the basics of CRISPR ribonucleoprotein (RNP) preparation and delivery for your next gene editing experiment.

Sept. 6, 2018, 12:02 p.m.

The CombiGEM technology allows a researcher to design and screen CRISPR gRNA libraries for effects on your biological phenomenon of choice.

April 12, 2016, 2:30 p.m.

Learn how to use in vitro screening and other tricks to expedite the CRISPR genome editing process in mice.

June 28, 2016, 10:59 a.m.

New CRISPR pooled libraries from the Broad Institute allow you to specifically target the mouse and human kinomes in your next screening experiment.

Sept. 9, 2016, 2:43 p.m.

Drive-and-proccess (DAP) arrays are a new tool from the Gao lab that allows for up to 31-loci CRISPR multiple base editing.

July 7, 2022, 1:15 p.m.

Find some tips and tricks for using CRISPR for gene editing.

March 7, 2017, 3:30 p.m.

Learn how to use Cas9 nickases for genome editing, including homology directed repair.

March 15, 2018, 12:59 p.m.

CRISPR-Sirius is a new tool to visualize genomic loci using dCas9. It's brighter than its predecessor CRISPRainbow and unlike fluorescent in situ hybridization, can be used in live cells.

April 18, 2019, 12:53 p.m.

Find out what CRISPR tools are distributed as a part of Addgene's new viral service.

Dec. 6, 2016, 3:30 p.m.

Review of off-target effects in CRISPR experiments. Topics covered include: measuring, preventing, and controlling for off-target events.

Feb. 8, 2024, 2:15 p.m.

Find information on CRISPR mammalian expression systems and delivery methods. Use the table to quickly compare between the methods.

Sept. 24, 2015, 2:30 p.m.