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We narrowed to 10 results for: grna

Showing: 1 - 10 of 10 results

  1. Plan Your Experiment

    Type
    Guide
    ...edit type, designing and cloning a gRNA, delivering Cas and the gRNA, and validating the edit.... system for Cas9 and the guide RNA (gRNA), the design of the gRNA sequence, and finally how to validate... proper gRNA design. While there are some cases where it makes sense to manually design a gRNA, in most...most cases gRNA design software is better and more efficient at designing potential gRNAs for you to choose...your gRNA is the balance between on-target and off-target activity. In a perfect world, your gRNA sequence...mismatches between a given gRNA sequence and your genomic target may reduce the gRNA activity. Be sure to sequence...sequence you used for gRNA design. Addgene offers many plasmids containing validated gRNAs . These plasmids...

  2. CRISPR Guide

    Type
    Guide
    ...optimized gRNA for your application (see: gRNA Design Software ). In addition to an optimized gRNA design... of unique gRNAs. gRNA design for CRISPR libraries is usually optimized to select for gRNAs with high ... guide RNA ( gRNA or sgRNA ) and a CRISPR-associated endonuclease (Cas enzyme). The gRNA is a short synthetic...commonly referred to as an sgRNA. gRNA scaffold sequence The sequence within the gRNA that is responsible for...amplify a specific sequence of DNA pegRNA P rime E dit gRNA; a specialized gRNA containing an additional primer...Cas enzyme and a gRNA specific to the gene of interest. The genomic target of the gRNA can be any ~20 nucleotide... between the gRNA scaffold and surface-exposed, positively-charged grooves on Cas9. gRNA binding induces...

  3. Guide to Using Pooled Libraries

    Type
    Guide
    ...each plasmid contains a unique cDNA. In shRNA or gRNA libraries, each plasmid contains a unique gene targeting...library from the Moffat lab has over 175,000 different gRNA-containing plasmids). Amplifying and Using the Library... screen. The figure above is an outline of how a gRNA library is used. Below are the general steps to ...experimental and control cells Generate a list of gRNAs that disappear with addition of the selection mechanism...

  4. Lentiviral Vector Guide

    Type
    Guide
    ... require gRNAs for each target. Lentiviral vectors are a common way of delivering these gRNAs, due to ...Vector Production Cloning Cloning your transgene, gRNA, or shRNA of interest into the transfer plasmid ...plasmids: Transfer plasmid — contains transgene, sgRNA, or shRNA of interest flanked by LTRs; 8–10 kb packaging...

  5. Modular Cloning Guide

    Type
    Guide
    ...-in-one CRISPR/Cas9 vectors expressing multiple gRNAs (up to seven) with a Cas9 nuclease, nickase, dCas9...plasmids including CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for genome editing in monocots...

  6. Addgene's eBook Collection

    Type
    Guide
    ...planning your CRISPR experiment and designing your gRNA and how to use pooled libraries in genome-wide screens...

  7. Adenovirus Guide

    Type
    Guide
    ...Vector Production Cloning Cloning your transgene, gRNA, or shRNA of interest into the transfer plasmid ...capacity is large enough to carry the Cas9 gene and a gRNA expression cassette in one single viral particle...

  8. Promoters

    Type
    Guide
    ...also transcribes small RNAs, such as shRNAs and gRNAs. Additional Resources Tetracycline (Tet) Inducible...

  9. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...Vector Production Cloning Cloning your transgene, gRNA, or shRNA of interest into the transfer plasmid ... Cas9 from Streptococcus pyogenes (SpCas9) and a gRNA together into an rAAV capsid is challenging due ...

  10. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...Vector Production Cloning Cloning your transgene, gRNA, or shRNA of interest into the transfer plasmid ...Figure 2): Transfer plasmid — contains transgene, sgRNA, or shRNA of interest flanked by LTRs; ~8 kb packaging...
Showing: 1 - 10 of 10 results