We narrowed to 202 results for: aav
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TypeBlog PostPublishedAug. 18, 2016, 2:30 p.m.... CRISPR/Cas9 Learn How CRISPR Has Been Used with AAV Resources on Addgene.org Find Cas9 Activators Find...
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Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog PostPublishedMarch 5, 2019, 1:08 p.m.... either by electroporation of plasmids, or using AAV vectors. The Imai lab also developed a version of... -
Antibodies 101: Single Chain Fragment Variables (scFvs)
TypeBlog PostPublishedJune 3, 2021, 1:15 p.m.... allows for their delivery by viral vectors like AAV, which could be useful for delivering scFvs that ... -
Plasmids 101: Expressing shRNAs for RNA Interference
TypeBlog PostPublishedJune 17, 2025, 1:15 p.m....occasions, shRNAs are transiently expressed using either AAV backbones or species-specific expression plasmids... -
Generating Mouse Models Using CRISPR/Cas9
TypeBlog PostPublishedJuly 12, 2016, 2:30 p.m....Acids Delivery, or GONAD (Gurumurty et al., 2016). AAV can also be used as a vector to deliver CRISPR/Cas9... -
Quick Guide to All Things Lentivirus
TypeBlog PostPublishedMarch 21, 2017, 2:30 p.m....Viral Transductions Learn Why You Might Want to Use AAV in Your Research Lentiviral Vector Uses and Overview... -
Plasmids 101: Using Transposons in the Lab
TypeBlog PostPublishedJuly 6, 2021, 1:28 p.m.... advantage over viral vectors (~5 kB payload for AAV and ~8 kB payload for lentivirus). Large cargos, ... -
How to Deposit Your Plasmids with Addgene
TypeBlog PostPublishedFeb. 27, 2024, 2:15 p.m....in bacteria) such as lentiviral, retroviral, and AAV plasmids, we recommend the NEB Stable strain. For... -
CRISPR 101: Multiplex Expression of gRNAs
TypeBlog PostPublishedSept. 10, 2020, 11:45 a.m....them advantageous for small capacity vectors like AAV. In addition to the mammalian option described below... -
Prime Editing: Adding Precision and Flexibility to CRISPR Editing
TypeBlog PostPublishedJan. 13, 2025, 9:49 p.m....respectively, to obtain PEs small enough to deliver with AAVs but still efficient at long and complex edits. The... -
Deep Dive: qPCR
TypeBlog PostPublishedSept. 29, 2022, 1:15 p.m....resources on the Addgene blog Digital Droplet PCR for AAV QuantificationPlasmid Cloning by PCR... -
Our New Antibody Mascot is....
TypeBlog PostPublishedFeb. 24, 2022, 2:15 p.m.... Last month, we introduced you to Blugene and Aavery's newest friend, our Addgene antibody mascot, here...on Feb. 24. Stay tuned! Original Blugene and Aavery would like your attention, please, as they introduce...AntibodyMakesThree on Twitter. As with Blugene and Aavery, we’re looking for a gender neutral name (and it... -
Typing CRISPR Systems
TypeBlog PostPublishedMarch 18, 2025, 1:15 p.m....362(6416), 839–842. https://doi.org/10.1126/science.aav4294 Hu, C., Myers, M. T., Zhou, X., Hou, Z., Lozen... 363(6422), 88–91. https://doi.org/10.1126/science.aav7271 Yoshimi, K., & Mashimo, T. (2022b). Genome ... -
Let There Be LITE Plasmids
TypeBlog PostPublishedNov. 21, 2013, 3:56 p.m....offers a tip: save yourself some time by making an AAV1 supernatant. (The details can be found here.) So... -
What's New in CRISPR - March 2020
TypeBlog PostPublishedMarch 10, 2020, 1:15 p.m....design and delivery. They generated hPSC lines with AAVS1-integrated, inducible, and fluorescent dCas9-KRAB... -
CRISPR 101: Cas9 Nickase Design and Homology Directed Repair
TypeBlog PostPublishedMarch 15, 2018, 12:59 p.m....situation thought to be suboptimal for HDR. At the AAVS1 locus, the two nearest gRNAs had cleavage sites ... -
Hot Plasmids - October 2022
TypeBlog PostPublishedOct. 6, 2022, 1:15 p.m....available as Cre-dependent, EF1ɑ-driven viral vectors (AAV1) - and you can also find several other constructs... -
Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2
TypeBlog PostPublishedMay 26, 2020, 1:15 p.m....editing at three different genomic sites in the AAVS1 locus using GEmCherry2. In this experiment they ... -
Plasmids 101: The protein expression toolbox
TypeBlog PostPublishedFeb. 16, 2023, 7:05 p.m....action Check out Addgene's Lentiviral Tet-on and AAVS1 targeted Tet-on transgene vector. Degron tags Tagging... -
PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog PostPublishedFeb. 23, 2016, 3:30 p.m....advantageous to adapt PITCh to insert genes into AAVS1, the “safe harbor locus” of the human genome, as...