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Showing: 1 - 20 of 48 results
  1. AAVED

    Type
    Collection
    ...about Addgene's 2018 AAV Education and Development (AAVED) workshop series....Development (AAVED) Series AAV Technologies and Methods for Targeted Neuronal Manipulation Meeting Date September... The AAV Education and Development...September 21, 2018 Contact Us Questions? Contact us at AAVED@addgene.org Sponsor We are grateful to The Kavli...brainstorm practical strategies for using combinations of AAV-based molecular tools to achieve targeted neuronal...Fan 9:30 AM Cre and Flpo Katherine Matho 10:15 AM AAV Retrograde Tracing Gowan Tervo and Sarada Viswanathan...Promoter/Enhancer Specificity, Serotype specificity, AAV dosing, and Other Forms of Regulation Ben Deverman...
  2. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...HEK293 cells) during AAV production. Read our AAV Guide for more information about: AAV components Common...112862 pAAV2/1 AAV1 AAV packaging plasmid, expressing Rep2 and Cap1 Wilson 104963 pAAV2/2 AAV2 AAV packaging...104964 pAAV2/5 AAV5 AAV packaging plasmid, expressing Rep2 and Cap5 Fan 112863 pAAV2/7 AAV7 AAV packaging...112864 pAAV2/8 AAV8 AAV packaging plasmid, expressing Rep2 and Cap8 Wilson 112865 pAAV2/9n AAV9 AAV packaging... Virus (AAV) Plasmids Additional Resources: AAV Guide Biosafety Guide All Viral Plasmids Blog: Plasmids...Vector Elements Blog: AAV use in CRISPR Viral Vectors 101 eBook Packaging Plasmids AAV Plasmids Adeno-associated...immunological effects in vivo . While AAVs can be handled at BSL-1, AAVs expressing oncogenes or toxins should...
  3. Retrograde AAV viral preps

    Type
    Collection
    ... Viral Service AAV Retrograde AAV Viral Service: Retrograde AAV As part of our Viral Service...catalog items. Browse Available Retrograde AAV See our retrograde AAV based on the functional catagories listed...Activity Category PI Controls 37825 AAV-CAG-GFP CAG GFP Control Boyden 51502 AAV pCAG-FLEX-EGFP-WPRE CAG EGFP...sensor Kim 104489 pGP-AAV-syn-jGCaMP7b-WPRE Syn GCaMP7b Calcium sensor Kim 104491 pGP-AAV-syn-FLEX-jGCaMP7s-WPRE...104493 pGP-AAV-syn-FLEX-jGCaMP7b-WPRE Syn GCaMP7b, Cre-dependent Calcium sensor Kim 104495 pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE...104496 pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE CAG GCaMP7f, Cre-dependent Calcium sensor Kim 104488 pGP-AAV-syn-jGCaMP7f-WPRE...104492 pGP-AAV-syn-FLEX-jGCaMP7f-WPRE Syn GCaMP7f, Cre-dependent Calcium sensor Kim 162374 GP-AAV-syn-jGCaMP8s-WPRE...
  4. Caltech Systemic Capsids

    Type
    Collection
    ...Broad tropism - AAV-PHP.eB, AAV-PHP.AX, AAV-PHP.eC Cell type-biased: Astrocytes - AAV-PHP.AX with GRE ...Neurons - AAV.CAP-B10, AAV-PHP.N Broad - AAV-PHP.AX with GRE or transgenic animal L5 & Inhibitory - AAV-PHP....and NHPs: Broad tropism - AAV-MaCPNS2, AAV-PHP.S Sensory nervous system - AAV-MaCPNS1 Return to top...pGP-AAV-syn-FLEX-jGCaMP7s-WPRE Syn Calcium sensor, Cre-dependent GCaMP Kim , GENIE 104492 pGP-AAV-syn-...pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE CAG Calcium sensor, Cre-dependent GCaMP Kim , GENIE 104496 pGP-AAV-CAG-...GENIE 104487 pGP-AAV-syn-jGCaMP7s-WPRE Syn Calcium sensor GCaMP Kim , GENIE 104488 pGP-AAV-syn-jGCaMP7f-WPRE...scientific community. Text Description for the AAV Decision Tree The AAV decision tree graphic above helps you ...
  5. AAV Made on Request

    Type
    Collection
    ... Viral Service AAV AAV Made on Request Viral...Viral Service: AAV Made on Request Due to high demand for viral items, Addgene's AAV Made on Request Service...Addgene is offering a Made on Request AAV packaging service for AAV plasmids not currently available as ...only offered for AAV plasmids deposited at Addgene. We are unable to perform custom AAV preparations for...available through the AAV Made on Request viral service? We only offer packaging for AAV plasmids deposited...service. AAV Service Details Volume 1-1.5 mL in 100 µL aliquots Minimum Titer 5E12 GC/mL Serotype AAV1, AAV2...Information on Addgene's AAV Made on Request viral packaging service Viral...
  6. Brain Initiative Collection

    Type
    Collection
    ...Addgene's AAV are made from a selected set of plasmids that are currently in our repository. AAV are distributed...coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA Ian Wickersham 104052-AAVPHP.V1 pAAV-CAG-DIO-EYFP An AAV genome...Viral Prep Description PI 65417-AAV8 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine AAV packaging vector for hSyn driven...enhancer element Gordon Fishell 99120-AAV1 pAAV-ihSyn1-tTA An AAV genome that expresses tTA from the hSyn1...of the tTA Viviana Gradinaru 99121-AAV1 pAAV-ihSyn1-DIO-tTA An AAV genome with Cre-dependent expression...of the tTA Viviana Gradinaru 99130-AAV1 pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized...6 enhancer Viviana Gradinaru 99130-AAV8 pAAV-mDlx-NLS-mRuby2 An AAV genome that expresses nuclear localized...
  7. Biosensor AAV Preps

    Type
    Collection
    ... Viral Service AAV Biosensors Viral Service: Biosensor AAV Browse our AAV that express fluorescent...162376 pGP-AAV-syn-jGCaMP8f-WPRE Syn jGCaMP8f none Constitutive 1, 9, rg* GENIE 162379 pGP-AAV-syn-FLEX-...162382 pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE CAG jGCaMP8f none Cre dependent 1, 5, 9, rg* GENIE 169258 AAV-hSyn-Soma-jGCaMP8f...176750 AAV-CamKIIa-jGCaMP8f-WPRE CamKIIa jGCaMP8f none Constitutive 1, 5, rg* Looger 176753 AAV-mDlx-jGCaMP8f-WPRE...162374 pGP-AAV-syn-jGCaMP8s-WPRE Syn jGCaMP8s none Constitutive 1, 5, 9, rg* GENIE 162377 pGP-AAV-syn-FLEX-jGCaMP8s-WPRE...162380 pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE CAG jGCaMP8s none Cre dependent 1, 9, rg* GENIE 169256 AAV-hSyn-Soma-jGCaMP8s...176752 AAV-CamKIIa-jGCaMP8s-WPRE CamKIIa jGCaMP8s none Constitutive 1, 9, rg* Looger 176755 AAV-mDlx-jGCaMP8s-WPRE...
  8. Serotype Testing AAV

    Type
    Collection
    ... Viral Service AAV Serotype Testing AAV Viral Service: ...: Serotype Testing AAV These AAV encode fluorescent reporters and can be used to compare the tropism of...experiments. For more information, see our full AAV inventory or our AAV production page . Note: For a list of ...Description : Ready-to-use AAV in various serotypes suitable for direct injection. These AAV are made from plasmid...have the suffix (for example, AAV1). AAV Vectors for Serotype Testing pAAV-CAG-GFP (Plasmid #37825) Description... 140 Add to Cart AAV Retrograde 37825-AAVrg.T 20 µL $ 140 Add to Cart pAAV-hSyn-EGFP (Plasmid #50465) ...
  9. Recombinases AAV Preps

    Type
    Collection
    ...provide high quality AAV preps from select plasmids in the repository. Browse our AAV collection of site... Viral Service AAV Recombinases Viral Service: Recombinases These AAV encode site-specific recombinases... gene expression. Dre AAV ID Name Promoter Fluorophore Serotype(s) PI 50363 AAV phSyn1(S)-DreO-bGHpA Syn...none 5, rg* Zeng Flpo AAV ID Name Promoter Fluorophore Serotype(s) PI 51669 AAV phSyn1(S)-FlpO-bGHpA hSyn...140137 pAAV-EF1a-iFlpV EF1a none 1, PHPeB Zeng *AAVrg = retrograde serotype, produced with the AAV retro...Deisseroth 87306 AAV pEF1a-DIO-FLPo-WPRE-hGHpA EF1a none 1, 2, 5, 8, 9, rg* Zhang 75469 pAAV-EF1a-Flp-DOG-...pAAV-EF1a-Flp-DOG-NW EF1a none 1 Cepko 183412 pAAV-CAG-FlpO CAG none 1, rg* Janelia VCre AAV ID Name Promoter Fluorophore...
  10. Cre-lox system

    Type
    Collection
    ...107312 AAV-hSyn-mCherry-P2A-Cre-WPRE mCherry and Cre; expressed in neurons hSyn AAV Yang 107313 AAV-aCamkII-mCherry-P2A-Cre-WPRE-BGH-polyA...Deisseroth 55635 pAAV-EF1a-sCre SCre EF-1 alpha AAV Deisseroth 55636 pAAV-EF1a-Cre Cre EF-1 alpha AAV Deisseroth...CamKII AAV Wilson 105553 pENN.AAV.hSyn.Cre.WPRE.hGH Cre hSyn AAV Wilson 105555 pENN.AAV.hSyn.Cre.hGH Cre ...51507 AAV pmSyn1-EBFP-Cre Cre-EBFP fusion; Expression in neurons. synapsin AAV Zeng 51904 paavCAG-iCre ...iCre CAG AAV Kim 55632 pAAV-Ef1a-mCherry-IRES-Cre Cre and mCherry coexpression EF-1 alpha AAV Deisseroth...EGFP-Cre fusion hSyn AAV Wilson 105545 pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 EGFP-Cre fusion CMV AAV Wilson 105550 ...Cre hSyn AAV Wilson 105558 pENN.AAV.CamKII 0.4.Cre.SV40 Cre CamKII AAV Wilson 105603 pAAV.GfaABC1D.PI.Cre.SV40...
  11. AAV Viral Preps

    Type
    Collection
    ...Serotypes If an AAV is available in serotypes AAV1, AAV2, AAV5, AAV6.2, AAV8, AAV9, or AAVrh10 it will be ... Viral Service AAV Viral Service: AAV Addgene's viral service provides high-quality...Samples small samples of control AAV vectors for trial purposes in serotypes AAV1, 2, 5, 8, 9 and retrograde...Ready-to-use adeno-associated virus (AAV) available from Addgene's viral service. Browse constructs currently...high-quality and high-titer viral preparations. Addgene's AAV are made from plasmids that are currently in our ...-use viral preparation from the inventory below. AAV are distributed as 100 µL solutions of purified viral... visit our Viral Service page . Browse Available AAV By Function Chemogenetics DREADDs PSAMs Optogenetics...
  12. Viral Production

    Type
    Collection
    ... and quality control are described below. AAV Production AAV distributed by Addgene has been produced ...determine purity of the AAV preparation. Silver staining of purified and non-purified AAV subjected to gel electrophoresis...using our virus protocols . Browse our AAV vectors collection. AAV Data Hub Beta Lentivirus Production Lentiviral... images). AAV Pro cells were transduced with either pAAV-Ef1a-mCherry-IRES-Cre (55634-AAVrg) alone at ... # 84445-AAVrg). Electron Microscopy The ratio of empty to full (i.e., genome containing) AAV particles... and AAV. Viral... Viral Service Viral Production Viral Production Jump To: Viral Production Overview AAV Production...
  13. Brain Armamentarium

    Type
    Collection
    ...PHPeB CN1390-rAAV-DLX2.0-minBG-SYFP2-WPRE3-BGHpA DLX2.0 (3xCore version of DLX enhancer). AAV vector for...Gradinaru 163509-PHPeB CN1839-rAAV-hSyn1-SYFP2-10aa-H2B-WPRE3-BGHpA AAV vector for nuclear SYFP2 labeling...Gradinaru 164450-PHPeB CN1851-rAAV-hI56i-minBglobin-iCre-4X2C-WPRE3-BGHpA AAV vector for Cre expression in...Jonathan Ting Viviana Gradinaru 37825-CAP-B10 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward...Edward Boyden Viviana Gradinaru 37825-CAP-B22 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward...Boyden Viviana Gradinaru 37825-CAP-MaCPNS1 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward...Boyden Viviana Gradinaru 37825-CAP-MaCPNS2 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward...
  14. Control AAV Preps

    Type
    Collection
    ... Viral Service AAV Controls Viral Service: Control AAV Browse our control AAV that express fluorescent...plasmids. Browse all Control AAV *AAVrg = retrograde serotype, produced with the AAV retro helper plasmid from...provide high quality AAV preps from select plasmids in the repository. Browse our control AAV collection, including...non-cre-dependent) Serotype AAV1 AAV2 AAV5 AAV8 AAV9 AAVrh10 Retrograde AAV PHP.eB PHP.S PHP.V1 CAP-B10...Constructs 45185 AAV-EF1a-BbTagBY EF1a TagBFP and EYFP Cre-dependent 9 Sanes 45186 AAV-EF1a-BbChT EF1a ....eB Roth 51506 AAV phSyn1(S)-tdTomato-WPRE hSyn tdTomato Constitutive 5 Zeng 58909 pAAV-GFAP104-mCherry...Roth 50459 pAAV-hSyn-DIO-mCherry hSyn mCherry Cre dependent 1, 2, 5, 8, 9, rg* Roth 51502 AAV pCAG-FLEX-EGFP-WPRE...
  15. AAV for Neuronal Tracing

    Type
    Collection
    ... Tracing These AAV encode tools for rabies virus-based monosynaptic tracing. These AAV can be used with...steps for using AAV and modified rabies virus (RABV) for monoclonal neuronal tracing. (1) AAV encoding TVA...deletion-mutant rabies virus AAV1 Wickersham 100798 pAAV-syn-FLEX-splitTVA-EGFP-tTA These AAV together can be used...virus. AAV Vectors for Monosynaptic Neuronal Tracing ID Name Description Serotype PI 52473 pAAV-synP-FLEX-splitTVA-EGFP-B19G...Ready-to-use AAV available from Addgene's viral service encoding tools for monosynpatic retrograde neuronal... Viral Service AAV Monosynaptic Neuronal Tracing Viral Service: Tools...to the starter cell in trans , via delivery by an AAV. Since this starter cell now has all the viral genes...
  16. Chemogenetics AAV Preps

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    Collection
    ...Chemogenetics Viral Service: Chemogenetics AAV Use our chemogenetics AAV to chemically induce neuronal activity...119742 AAV SYN PSAM4 GlyR IRES EGFP PSAM4 GlyR - Inhibition IRES EGFP none 5 Sternson 119744 AAV CAMKII...Constitutive (non-cre dependent) Serotype AAV1 AAV2 AAV5 AAV8 AAV9 Retrograde AAV PHPeB Clear Filters ID Name Receptor..., 9, PHPeB Dimidschstein *AAVrg = retrograde serotype, produced with the AAV retro helper plasmid from...Ready-to-use AAV available from Addgene's viral service encoding chemogenetics tools. Viral... Viral Service AAV Chemogenetics...mCherry fusion Flp-dependent 8, rg* Gether 119741 AAV SYN flex PSAM4 GlyR IRES EGFP PSAM4 GlyR - Inhibition...
  17. AAV Molecular Tools

    Type
    Collection
    ...Ready-to-use AAV available from Addgene's viral service encoding tet-off transactivators and tools for... Viral Service AAV Molecular Tools Viral Service: Molecular Tools Tetracycline...Tetracycline Transactivators and Inducible Tools These AAV encode tetracycline-inducible tools/controls and ... Gradinaru Tools for Affinity Purification These AAV encode tools for affinity purification (which can...Schmidt Tools for Neuronal Connectivity Labeling These AAV encode tools that can be used for neuronal connectivity...Name Expression System Activity Serotype PI 51509 AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE Syn-driven...axon terminals. 1 Luo Tools for Cell Ablation These AAV encode tools that can be used for cell ablation. ...
  18. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ...save you time and help you get results faster. AAV AAV are distributed as 100 µL of purified viral particles...Viral preparation options include lentivirus and AAV. Viral... are reported with each shipment. Find Available AAV Lentivirus Lentiviruses are distributed as purified...reports from the research community demonstrating AAV performance under various experimental conditions... tecnologies. Penn Vector Core Transfer Status : AAV vectors from the Penn Vector Core will be transferred...
  19. Zhang Lab CRISPR Page

    Type
    Collection
    ...Posts Jump to: SpCas9 Plasmids GeCKO SAM AAV - In vivo Delivery AAV - Cas9 Mouse CRISPR ( C lustered R egularly... lung adenocarcinoma. Delivery of a single AAV vector ( AAV-KPL ) in the lung generated loss-of-function...nature14299. Epub 2015 Apr 1. PubMed . Return to AAV In vivo plasmids AAV Plasmids with Cas9 Mouse CRISPR-Cas9 knockin...#60958) contains pAAV-U6::sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). This is a AAV plasmid for sgRNA...This plasmid is used as a control for AAV-KPL. #60226 - AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR... Lab SAM Cloning Protocol 321.5 KB Return to top AAV - In vivo Genome Editing The Zhang Lab has both single...Cas9 and sgRNA in vivo via adeno-associated virus (AAV). The single vector system uses a smaller Cas9 from...
  20. Optogenetics AAV Preps

    Type
    Collection
    ...options. Browse all Control AAV *AAVrg = retrograde serotype, produced with the AAV retro helper plasmid from...high quality AAV preps from select plasmids in the repository. Browse our optogenetics AAV collection, ... Viral Service AAV Optogenetics Viral Service: Optogenetics AAV Optogenetic tools can be used...20071 pACAGW-ChR2-Venus-AAV CAG ChR2 Venus Constitutive 1, 9 Svoboda 83898 pAAV-mDlx-ChR2-mCherry-Fishell... 28017 AAV-CAG-hChR2-H134R-tdTomato CAG ChR2/H134R tdTomato Constitutive rg* Svoboda 75470 pAAV-CAG-FLEXFRT-ChR2...62723 pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] Syn ChrimsonR tdTomato Cre dependent 5 Boyden 130909 AAV-CAG-...and variants 22222 AAV-FLEX-Arch-GFP CAG Arch GFP Cre dependent 1, 9 Boyden 28305 pAAV-FLEX-ArchT-tdTomato...
Showing: 1 - 20 of 48 results