Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 484 results for: multi

Showing: 181 - 200 of 484 results

  1. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    Published
    March 1, 2016, 3:30 p.m.
    ...assembly is well known for allowing easy assembly of multiple linear DNA fragments, but can also be used in ...biology, mainly because of the ease in assembling multiple fragments in one step with no scar sequences remaining...Whether it is for routine cloning, assembly of multiple fragments, or synthetic biology, you should consider...

  2. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...is necessary or desirable to control or look at multiple genes at the same time.  The 3 binary systems ...analysis in Drosophila or for precise control over multiple genes in synthetic systems. Recent work has even... Multiple Authors...

  3. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...about 100 bps into the run, it got “dirty”, with multiple sequence traces overlapping each other! When the... Wu X, Jiang W, Marraffini LA, Zhang F (2013) Multiplex Genome Engineering Using CRISPR/Cas Systems. Science...-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering...

  4. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...counts making them fast and viable options for multiplex sequencing in many laboratories. Nevertheless,...DSBs, doesn't require high read counts, fast multiplexing Requires delivery of dsODN (potentially toxic...dsODN), doesn't require high read counts (fast multiplexing) Requires large cell population, senstive to...

  5. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ... protein because the antibodies will recognize multiple epitopes on the protein. However, each animal ...advantage of flow cytometry is the ability to use multiple fluorescent signals (up to a dozen or more!) to...relative or absolute amount of the expression of multiple proteins on the same cell simultaneously as well...

  6. Lucky Thirteen: Guest Contributors of 2023

    Type
    Blog Post
    Published
    Dec. 5, 2023, 2:15 p.m.
    ...KarplusIBEX Knowledgebase: A Data Resource for Multiplex Tissue ImagingResearchers: Andrea Radtke, Ziv ...Cynthia Arokiaraj; Viviana Gradinaru; Tim ShayMultifunctional Neuronal Fibers: Modulation from Gut to BrainResearchers...

  7. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...way to increase CRISPR editing efficiency across multiple CRISPR enzymes. Plasmids for CRISPR coselection...segment on regulating telomere length with TZAP Multiplexible plant genome engineering toolkit The Voytas ... well as Cys4, tRNA, and ribozyme systems for multiplex gRNA expression. Expression of toolkit components...

  8. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    Published
    Dec. 18, 2018, 1:17 p.m.
    ... they have developed this further to provide multiplexed detection, higher sensitivity, and more. Read...lab compared the efficiency and specificity of multiple wild-type and engineered variants of Cas nucleases...

  9. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    Published
    Nov. 10, 2015, 3:30 p.m.
    ... which display sophisticated interactions with multiple other proteins. Again, little to no change in ... place to start. These tags are easy to use in multiple common applications. Due to their limited effects...

  10. Developing Transferable Skills During Science Training

    Type
    Blog Post
    Published
    June 3, 2014, 2:14 p.m.
    ...less experienced grads Manage a project involving multiple scientists, create and stick to timelines Be a...another field maybe, and publish Join a lab that has multiple collaborations, even with Industry labs Work on...

  12. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    Published
    May 17, 2016, 2:30 p.m.
    ...e.g. SNPs) exist. Additionally, consider testing multiple gRNAs targeted to the same locus to ensure their...elegans gRNA sequences. You can also filter by multiple keywords that are separated by a single space ...

  13. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    Published
    June 19, 2015, 3:08 p.m.
    ...protein purification through simple and efficient multiplexing. pCri plasmids are divided into two sets, each...schematic below), enabling a researcher to test multiple tags for one protein quickly and easily. These...

  14. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    Published
    Dec. 12, 2023, 2:15 p.m.
    ...both colors allows for increased options for multiplexing in experiments, including ones that study alpha2...Reiner, A., … Patriarchi, T. (2023). Sensitive multicolor indicators for monitoring norepinephrine in vivo...

  15. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    Published
    March 27, 2014, 5:14 p.m.
    ....1918.) Near-infrared fluorescent proteins for multicolor in vivo imaging. Shcherbakova and Verkhusha (...10;4:2153. doi: 10.1038/ncomms3153. PubMed) Multicontrast photoacoustic in vivo imaging using near-infrared...

  16. Fostering communication in remote collaborations

    Type
    Blog Post
    Published
    April 21, 2020, 1:14 p.m.
    ...as Slack. Share data and documents While having multiple scientists edit the same document may sound like...collaborators. Being able to present data from multiple aspects of a collaboration and immediately discuss...

  17. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ...for selection of plasmid-containing bacteria. Multiple Cloning Site (MCS) Short segment of DNA which ...maintain your plasmid stocks. The guide also contains multiple protocols and troubleshooting tips to make plasmid...

  18. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    Published
    May 2, 2023, 1 p.m.
    ...Development-specific genes – if a gene functions at multiple stages in development, its depletion may result...be observed. Functionally redundant genes – multiple genes which function in overlapping roles can ...

  19. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...frequently used in molecular biology to isolate, multiply, or express the insert they carry in the target...has the CMV promoter for mammalian expression, a multiple cloning site to insert YGOI, a bacterial selection...

  20. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ...biosynthetic pathways. Biosynthetic pathways containing multiple enzymes could be successfully reconstituted in.... The lab demonstrated that XMAS-TREE can be multiplexed and can be used in human pluripotent stem cells...
Showing: 181 - 200 of 484 results