...journal club discussion on Kevin Esvelt’s CRISPR/Cas9 gene drive paper was very popular. People kept asking...

...end-point sequencing assays. These plasmids use “Cas9-mediated adenosine transient reporter for editing...

...) model consists of a catalytically dead Cas9 protein (dCas9) that lacks endonuclease activity coupled.... Using dCas9 as an on/off switch The multiple scRNAs all individually utilize the same dCas9 protein....modulate dCas9 expression, multiple scRNA transcriptional programs can be regulated with dCas9 as a master...an orthogonal dCas9 protein that will still recognize guide RNA but with a different dCas9 binding module...specific proteins via aptamer domains. Combined with dCas9, this new scaffold RNA (scRNA) will bind to a PAM-adjacent...layered with distinct programs controlled by each dCas9 regulatory switch. By no means limited to the field...applied to effectively change metabolic flux. The dCas9 protein and orthogonal variants can be used as regulatory...

... EGFP and mEGFP. Many of these features, such as Cas9, weren’t detected by our in-house software. In fact...

... endogenous protein fusions created using CRISPR/Cas9. eNLs offer high luminescent signal for imaging ...

...depositors have developed heat shock-inducible Cre and Cas9 for easy genome engineering in species like C. elegans...

...primer for beginners Adenoviral delivery of CRISPR/Cas9 Tips for a first time AAV user Resources on Addgene.org...

...changes and require updating or retiring. CRISPR/Cas9, for example, has gone through rapid change in the...

... Genetic screens in human cells using the CRISPR/Cas9 system. Science, 343(6166): 80-84 (2014). 10.1126...

...Th2) cells from mice that constitutively express Cas9 and screen for genes that affect Th2 differentiation...Wang Lab created a tripartite system consisting of dCas9, a gRNA fused to an RNA binding motif, and a RNA-binding...protein-bEFP fusion. Thus, the bEFPs bind the UAS via the dCas9-gRNA complex which allows transcription from the...

...used with many types of proteins, including Cas9 and dCas9-transcription factors, for which low expression...

...translation in living single cells. However, before CRISPR-Cas9 these techniques were quite tedious and, even here...

...versions of non-modular proteins like Cre. Could Cas9-DOG be developed soon to allow genome modification...

...single 72h harvest is best for larger genes such as Cas9. See figure 1 for an example of how changing transfection...

...community has created hundreds of useful variants of Cas9. By providing access to the plasmids encoding antibodies...

..., Sanjana NE, Zhang F (2017) Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening...

...amplification, new toehold switch designs, CRISPR/Cas9-mediated genotyping, as well as our electronic reader...

...well plate for further analysis. To assess CRISPR/Cas9 knockout efficacy, we have used both Western blotting...

...primer for beginners Adenoviral delivery of CRISPR/Cas9 5 tips for troubleshooting viral transductions ...

...   For CRISPR libraries, Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening...