We narrowed to 486 results for: multi
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  TypeBlog PostPublishedSept. 21, 2021, 1:15 p.m....biosynthetic pathways. Biosynthetic pathways containing multiple enzymes could be successfully reconstituted in.... The lab demonstrated that XMAS-TREE can be multiplexed and can be used in human pluripotent stem cells...
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  Even more elegant: Single injection CRISPR/Cas9 in C. elegansTypeBlog PostPublishedJuly 7, 2015, 3:36 p.m....modular, selection-based gene tagging approach Multiple features of C. elegans have made CRISPR/Cas9 so...HDR mentioned above, an accessible germline, and multiple selectable markers. Bob Goldstein’s lab previously...
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  Another Pathway into Cells: iTOPTypeBlog PostPublishedJune 23, 2015, 8:37 p.m....efficient in primary fibroblasts. iTOP also works in multiple types of primary mouse cells, including neuronal...and continued protein expression would require multiple transduction events. For these reasons, iTOP is...
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  Extracurricular Activities for a Strong Science Career PathTypeBlog PostPublishedJan. 21, 2014, 3:08 p.m....graduate and postdoc training focuses on using multidisciplinary, collaborative, and translational approaches...word “extracurricular” may be taboo in the lab, multiple research studies highlight the importance of taking...
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  Using CRISPR/Cas9 to Edit Disease Out of the GenomeTypeBlog PostPublishedJune 23, 2022, 1:15 p.m.... therapy, however, because the disease affects multiple organ systems. Nevertheless, the findings are ... Multiple Authors...
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  Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UASTypeBlog PostPublishedJuly 21, 2017, 12:48 p.m....Controlling multiple genes with Gal4/UAS system One can also use Gal4 to drive expression of multiple genes ...
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  FlipGFP, a novel fluorescence protease reporter to study apoptosisTypeBlog PostPublishedMay 21, 2019, 12:10 p.m....to create an array of multicolor reporters that will enable imaging of multiple proteases at once (Zhang...
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  AAV Q&A with Tim MilesTypeBlog PostPublishedMay 17, 2022, 1:15 p.m.... will very likely cross-react with each other. Multiple labs have tried to transfer PHP.eB's enhanced ...which may be linked to epigenetic changes, such as multiple sclerosis? A: Basic science and preclinical researchers...
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  A Practical Approach to Choosing the B(right)est Fluorescent ProteinTypeBlog PostPublishedJune 1, 2017, 2:30 p.m.... The theoretical brightness is calculated by multiplying EC by QY (EC*QY, sometimes normalized to the ...I Use? Choosing Your Fluorescent Proteins for Multicolor Imaging Learn about Fluorescent Protein Biosensors...
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  Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and TransfectionTypeBlog PostPublishedJune 25, 2019, 12:54 p.m....molecular biology and scientific research. There are multiple ways foreign DNA can be introduced into cells ...References Baltrus, David A., and Karen Guillemin. "Multiple phases of competence occur during the Helicobacter...
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  Targeted Mutagenesis with EvolvRTypeBlog PostPublishedFeb. 21, 2019, 1:15 p.m....diversity introduced by lambda red recombineering and Multiplex Automated Genome Engineering (MAGE) will always...progeny of a single starting cell. Additionally, multiplexing of guides allows EvolvR to target more than ...
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  Quick Guide to Working with Drosophila Part 3: Genome Engineering in FliesTypeBlog PostPublishedJuly 28, 2017, 1:30 p.m....Also, if there is a chance you need to combine multiple transgenes in one fly, having the transgenes in...designing experiments where you want to express multiple transgenes, remember that you’ll need to integrate...
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  Viral Vectors 101: BiosensorsTypeBlog PostPublishedJuly 6, 2023, 1 p.m.... have been tested in vivo, in tissue preps, in multiple species, and in cell culture models. Some of them... them have even been expanded for multiplexed imaging for advanced monitoring of different factors, in...
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  Viral Vectors 101: Types of virusesTypeBlog PostPublishedJune 6, 2023, 1:15 p.m....administration, such as in mouse experiments. AAV also has multiple serotypes (12 naturally occurring and more synthetic...enough for many applications, but in some cases multiple AAVs (co-infection), an alternative virus, or ...
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  Viral Vectors 101: Viruses as Biological ToolsTypeBlog PostPublishedMarch 21, 2023, 1:15 p.m....therapies have already been approved in the US for multiple diseases, including hemophilia B and spinal muscular... Multiple Authors...
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  How to Keep a Lab Notebook for Bioinformatic AnalysesTypeBlog PostPublishedJune 2, 2016, 2:30 p.m....folder: You will often use the same data files for multiple analyses. Rather than copying these files to your...to using dates as labels, since I often work on multiple projects in a given day. For example, instead ...
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  Antibodies 101: ConjugationTypeBlog PostPublishedMarch 26, 2024, 1:15 p.m.... the visible spectrum, so they are great for multiplexing and can be used in a variety of assay types....detection of low abundance targets with significant multiplexing potential. You will find antibody-oligonucleotide...
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  Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogeneticsTypeBlog PostPublishedMarch 3, 2020, 2:15 p.m.... Type IIS restriction sites to easily assemble multiple DNA fragments. This system has been used to construct...controlled by Cre recombination. Each cell can have multiple copies of the transgene that recombine independently...
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  Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression ToolsTypeBlog PostPublishedApril 23, 2018, 2 p.m....aberrantly activate the proteasome. Clift et al. tested multiple methods for TRIM-Away delivery. For small cell...FIRE-Cas9, experiments can be designed to target multiple loci at once. In addition, the approach allows...
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  dTAG - You're it!TypeBlog PostPublishedJune 21, 2018, 2:06 p.m.... transcription factors with important roles in Multiple Myeloma), it is important to ensure lack of degradation...system was employed to evaluate degradation of multiple FKBP12F36V-tagged proteins using our lentiviral...