We narrowed to 484 results for: multi
-
TypeBlog PostPublishedJuly 7, 2015, 3:36 p.m....modular, selection-based gene tagging approach Multiple features of C. elegans have made CRISPR/Cas9 so...HDR mentioned above, an accessible germline, and multiple selectable markers. Bob Goldstein’s lab previously...
-
Another Pathway into Cells: iTOP
TypeBlog PostPublishedJune 23, 2015, 8:37 p.m....efficient in primary fibroblasts. iTOP also works in multiple types of primary mouse cells, including neuronal...and continued protein expression would require multiple transduction events. For these reasons, iTOP is... -
Extracurricular Activities for a Strong Science Career Path
TypeBlog PostPublishedJan. 21, 2014, 3:08 p.m....graduate and postdoc training focuses on using multidisciplinary, collaborative, and translational approaches...word “extracurricular” may be taboo in the lab, multiple research studies highlight the importance of taking... -
FlipGFP, a novel fluorescence protease reporter to study apoptosis
TypeBlog PostPublishedMay 21, 2019, 12:10 p.m....to create an array of multicolor reporters that will enable imaging of multiple proteases at once (Zhang... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog PostPublishedJuly 21, 2017, 12:48 p.m....Controlling multiple genes with Gal4/UAS system One can also use Gal4 to drive expression of multiple genes ... -
Using CRISPR/Cas9 to Edit Disease Out of the Genome
TypeBlog PostPublishedJune 23, 2022, 1:15 p.m.... therapy, however, because the disease affects multiple organ systems. Nevertheless, the findings are ... Multiple Authors... -
Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection
TypeBlog PostPublishedJune 25, 2019, 12:54 p.m....molecular biology and scientific research. There are multiple ways foreign DNA can be introduced into cells ...References Baltrus, David A., and Karen Guillemin. "Multiple phases of competence occur during the Helicobacter... -
Targeted Mutagenesis with EvolvR
TypeBlog PostPublishedFeb. 21, 2019, 1:15 p.m....diversity introduced by lambda red recombineering and Multiplex Automated Genome Engineering (MAGE) will always...progeny of a single starting cell. Additionally, multiplexing of guides allows EvolvR to target more than ... -
Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies
TypeBlog PostPublishedJuly 28, 2017, 1:30 p.m....Also, if there is a chance you need to combine multiple transgenes in one fly, having the transgenes in...designing experiments where you want to express multiple transgenes, remember that you’ll need to integrate... -
A Practical Approach to Choosing the B(right)est Fluorescent Protein
TypeBlog PostPublishedJune 1, 2017, 2:30 p.m.... The theoretical brightness is calculated by multiplying EC by QY (EC*QY, sometimes normalized to the ...I Use? Choosing Your Fluorescent Proteins for Multicolor Imaging Learn about Fluorescent Protein Biosensors... -
AAV Q&A with Tim Miles
TypeBlog PostPublishedMay 17, 2022, 1:15 p.m.... will very likely cross-react with each other. Multiple labs have tried to transfer PHP.eB's enhanced ...which may be linked to epigenetic changes, such as multiple sclerosis? A: Basic science and preclinical researchers... -
Viral Vectors 101: Biosensors
TypeBlog PostPublishedJuly 6, 2023, 1 p.m.... have been tested in vivo, in tissue preps, in multiple species, and in cell culture models. Some of them... them have even been expanded for multiplexed imaging for advanced monitoring of different factors, in... -
Viral Vectors 101: Types of viruses
TypeBlog PostPublishedJune 6, 2023, 1:15 p.m....administration, such as in mouse experiments. AAV also has multiple serotypes (12 naturally occurring and more synthetic...enough for many applications, but in some cases multiple AAVs (co-infection), an alternative virus, or ... -
Viral Vectors 101: Viruses as Biological Tools
TypeBlog PostPublishedMarch 21, 2023, 1:15 p.m....therapies have already been approved in the US for multiple diseases, including hemophilia B and spinal muscular... Multiple Authors... -
How to Keep a Lab Notebook for Bioinformatic Analyses
TypeBlog PostPublishedJune 2, 2016, 2:30 p.m....folder: You will often use the same data files for multiple analyses. Rather than copying these files to your...to using dates as labels, since I often work on multiple projects in a given day. For example, instead ... -
dTAG - You're it!
TypeBlog PostPublishedJune 21, 2018, 2:06 p.m.... transcription factors with important roles in Multiple Myeloma), it is important to ensure lack of degradation...system was employed to evaluate degradation of multiple FKBP12F36V-tagged proteins using our lentiviral... -
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog PostPublishedApril 23, 2018, 2 p.m....aberrantly activate the proteasome. Clift et al. tested multiple methods for TRIM-Away delivery. For small cell...FIRE-Cas9, experiments can be designed to target multiple loci at once. In addition, the approach allows... -
Troubleshooting Your Plasmid Cloning Experiment
TypeBlog PostPublishedSept. 24, 2019, 12:55 p.m....methylation like XbaI, ClaI, etc. If you are doing multiple digestion be sure that the buffers and temperature...molar ratio. Assembly of the fragments There are multiple ways you can assemble the different parts of a... -
Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics
TypeBlog PostPublishedNov. 3, 2020, 2:15 p.m....electric current of ion channels in living cells. Multiple properties of a channelrhodopsin, such as cell... as compared to direct injection. This lower multiplicity of infection (MOI) makes it challenging for ... -
Antibodies 101: Conjugation
TypeBlog PostPublishedMarch 26, 2024, 1:15 p.m.... the visible spectrum, so they are great for multiplexing and can be used in a variety of assay types....detection of low abundance targets with significant multiplexing potential. You will find antibody-oligonucleotide...