We narrowed to 254 results for: cas9

Some promoters are only active under specific circumstances. Here's a guide to inducible promoters, which are regulated by chemicals, light, and/or temperature.

Jan. 18, 2018, 2:34 p.m.

Using synthetic biology principles has simplified and improved plasmids for optogenetic control of gene expression in E. coli.

Sept. 8, 2015, 2:30 p.m.

A general overview of how and when to use screens vs. selections and the ins and outs of each method.

Nov. 10, 2022, 2:15 p.m.

Tim Miles, the guest speaker at our recent AAV webinar, and our webinar panel of experts, is finishing up their Q&A session.

May 17, 2022, 1:15 p.m.

We've partnered with SnapGene to update our plasmid maps. Learn how to use our new maps to accelerate your research.

June 29, 2017, 1:06 p.m.

Nano-lanterns contain luciferase fused to a fluorescent protein. The Nagai lab has assembled multicolored nano-lanterns for various applications.

May 25, 2017, 2:30 p.m.

Learn how you can visualize newly synthesized proteins with FUNCAT-PLA.

Jan. 16, 2018, 3:20 p.m.

Connie Cepko's lab at Harvard University has developed transcription factor and CRE recombinase variants activated by GFP. Learn more inside!

Nov. 24, 2015, 3:30 p.m.

Plasmid-encoded antibodies offers many advantages over other types of antibodies. Learn about how recombinant antibodies are made.

May 4, 2021, 1:15 p.m.

Find tips and best practices for producing lentivirus in your lab: from choosing a packaging system to optimizing transfection and improving titers.

Aug. 7, 2018, 12:31 p.m.

Researchers from the Collins and Yin labs have developed new paper-based diagnostics for detecting Zika. Learn more inside.

Aug. 30, 2016, 2:30 p.m.

Once you create a pooled library, you'll need to ensure it has an amplification protocol. Read our blog on how to develop a protocol for your library!

May 30, 2024, 1:30 p.m.

An overview of split fluorescent proteins and a highlight of some of the most popular and newest ones available on Addgene.

May 3, 2022, 1:15 p.m.

All new plasmids deposited at Addgene are verified by next-generation sequencing to provide you with more data to choose the right plasmid for your next experiment.

April 6, 2021, 12:30 p.m.

Learn how to produce, purify, and titer adenoviral vectors for your research.

June 14, 2018, 11:44 a.m.

Quickly isolate mammalian cells clones using the agarose cloning method.

Sept. 7, 2017, 12:17 p.m.

This month's Hot Plasmids articles features new plasmids, new antibodies, and a viral ticker tape system!

Nov. 14, 2023, 3:35 a.m.

Pooled libraries are a powerful tool, but they can difficult to prepare! Learn the tips and tricks for taking your pooled libraries from plasmids to screening.

July 13, 2023, 1:15 p.m.

A recent study shows ancestral bias in CRISPR guide libraries, leading to skewed cancer dependency results, particularly in cell lines with African ancestry.

Nov. 29, 2022, 2:15 p.m.

REPAIR and RESCUE are two CRISPR tools for RNA editing based on Cas13b.

July 31, 2020, 12:30 p.m.