We narrowed to 253 results for: cas9

1. Overcoming the Challenges of Lentiviral Production

   Type

   Blog Post

   Published

   Aug. 7, 2018, 12:31 p.m.

   ...single 72h harvest is best for larger genes such as Cas9. See figure 1 for an example of how changing transfection...

2. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

   Type

   Blog Post

   Published

   May 4, 2021, 1:15 p.m.

   ...community has created hundreds of useful variants of Cas9. By providing access to the plasmids encoding antibodies...

3. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

   Type

   Blog Post

   Published

   April 6, 2021, 12:30 p.m.

   ..., Sanjana NE, Zhang F (2017) Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening...

4. Adenoviral Vector Production and Troubleshooting

   Type

   Blog Post

   Published

   June 14, 2018, 11:44 a.m.

   ...primer for beginners Adenoviral delivery of CRISPR/Cas9 5 tips for troubleshooting viral transductions ...

5. Cloning Mammalian Cells with the Agarose Method

   Type

   Blog Post

   Published

   Sept. 7, 2017, 12:17 p.m.

   ...well plate for further analysis. To assess CRISPR/Cas9 knockout efficacy, we have used both Western blotting...

6. Pooled Library Amplifications

   Type

   Blog Post

   Published

   May 30, 2024, 1:30 p.m.

   ...   For CRISPR libraries, Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening...

7. Uncovering Ancestral Bias in CRISPR Research

   Type

   Blog Post

   Published

   Nov. 29, 2022, 2:15 p.m.

   ... the guide from binding to the genome or prevent Cas9 from cutting, both of which can introduce significant...

8. Split Fluorescent Proteins for Studying Protein-Protein Interactions

   Type

   Blog Post

   Published

   May 3, 2022, 1:15 p.m.

   ...Split-horseradish peroxidase (Alice Ting Lab 2016)  Split-Cas9 for inducible genome editing (Feng Zhang Lab 2015...

9. Hot Plasmids - November 2023

   Type

   Blog Post

   Published

   Nov. 14, 2023, 3:35 a.m.

   ...edits, such as long or highly structured templates. Cas9 variants PE6e–g often had even higher editing efficiencies...

10. Lentiviral Vector Uses and Overview

    Type

    Blog Post

    Published

    May 19, 2016, 2:30 p.m.

    ...Screening Read about Genome-Wide Screening Using CRISPR/Cas9 Additional Resources on Addgene.org Check out ...

11. Tips for arabidopsis transformation

    Type

    Blog Post

    Published

    Oct. 25, 2018, 1:23 p.m.

    ... blog Engineering the plant genome using CRISPR/Cas9 Using pSiM24 for simplifying plant genetic engineering...

12. CRISPR 101: RNA Editing with Cas13

    Type

    Blog Post

    Published

    July 31, 2020, 12:30 p.m.

    ...the target locus, making them more flexible than Cas9/Cpf1. Some Cas13 enzymes prefer targets with a given...

13. The Fluorescent Vegetables in Aptamer Soup

    Type

    Blog Post

    Published

    Jan. 29, 2019, 2:27 p.m.

    ...cellular RNA to specific intracellular locations using Cas9 and track the localization of the complex. Broccoli...

14. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type

    Blog Post

    Published

    April 24, 2015, 2:39 p.m.

    ...CRISPR-Cas9 genome editing may be the hot new way to manipulate gene expression, but other gene manipulation...

15. Technologies Enabled by NanoLuc® Luciferase

    Type

    Blog Post

    Published

    Feb. 8, 2018, 12:17 p.m.

    ...endogenous tagged genes generated through CRISPR/Cas9 knock-in. Finally, NLuc is very inviting for endogenous...

16. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type

    Blog Post

    Published

    Sept. 1, 2020, 1:15 p.m.

    ...sequential homology directed repairs (HDR) with CRISPR/Cas9. Donor A contains: 400bp homology arms to the site...

17. Quick Guide to All Things Lentivirus

    Type

    Blog Post

    Published

    March 21, 2017, 2:30 p.m.

    ...vectors fantastic options for delivering shRNA, CRISPR/Cas9 components, and fluorescent sensors. Retroviral...

18. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type

    Blog Post

    Published

    Oct. 27, 2020, 1:15 p.m.

    ...Bassik MC (2016) Systematic comparison of CRISPR/Cas9 and RNAi screens for essential genes. Nat Biotechnol...

19. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type

    Blog Post

    Published

    Aug. 15, 2017, 1:24 p.m.

    ...other technologies such as optogenetics and CRISPR/Cas9 will help immunologists create even better tools...

20. How to Deposit Your Plasmids with Addgene

    Type

    Blog Post

    Published

    Feb. 27, 2024, 2:15 p.m.

    ...cloned into it. gRNAs are used for directing CRISPR/Cas9 to a targeted genomic location, while shRNAs are...