We narrowed to 371 results for: crispr

Here, we cover four different plasmid screening strategies including blue-white screening, colony PCR, restriction digest analysis, and Sanger sequencing.

Sept. 15, 2020, 1:15 p.m.

Read about the iGEM IISER Bhopal team and their work developing a cold tolerant E. coli using specialized chaperones.

Jan. 28, 2020, 2:35 p.m.

We've partnered with SnapGene to update our plasmid maps. Learn how to use our new maps to accelerate your research.

June 29, 2017, 1:06 p.m.

Learn how dry ice shipment can affect pH and discover precautions you can take to protect your virus samples.

June 20, 2017, 2:30 p.m.

Learn how to determine the practical brightness of your fluorescent protein in your specific experimental setup.

June 1, 2017, 2:30 p.m.

Nano-lanterns contain luciferase fused to a fluorescent protein. The Nagai lab has assembled multicolored nano-lanterns for various applications.

May 25, 2017, 2:30 p.m.

Learn about click biology and how you can use it to advance your experiments and explore biological phenomenon.

Oct. 21, 2025, 1:15 p.m.

Adeno-associated Virus (AAV) is used extensively for in-vivo transgene expression. Learn how to use AAV in this post.

May 30, 2023, 1:15 p.m.

There are many tools available for studying the microbiome. Learn about plasmid tools for engineering gut microbes, detection, and synthetic biology.

June 27, 2019, 12:25 p.m.

Gene expression is often imprecise due to variations in gene copy number within the cell (either on plasmid or genome). New promoters from Chris Voigt’s lab enable stable gene expression regardless of copy number regardless of growth phase and whether they are expressed on the plasmid or chromosome.

Sept. 4, 2018, 12:53 p.m.

Congratulations to Paula Videira, Michael McMurray, & Richard Gronostajski who each are being awarded free plasmids form Addgene. Read on to learn how these researchers plan to use plasmids for research on diseases of glycosylation, arginine mutagenesis, & neural development.

June 26, 2018, 1:33 p.m.

Learn how you can visualize newly synthesized proteins with FUNCAT-PLA.

Jan. 16, 2018, 3:20 p.m.

Addgene Software Engineer, Kris Shamloo, describes difficulties we've faced developing our lab management software and gives tips to help you make your own.

Aug. 25, 2016, 2:30 p.m.

It's important to determine how much functional virus you have a in your prep before starting a transduction. Learn how to titer your lentivirus here.

March 15, 2016, 2:30 p.m.

Plasmid-encoded antibodies offers many advantages over other types of antibodies. Learn about how recombinant antibodies are made.

May 4, 2021, 1:15 p.m.

Overwhelmed by too many papers to read? Find four ways to search for and save relevant papers.

Jan. 9, 2020, 1:45 p.m.

If you're new to behavioral neuroscience, this article will walk you through how to standardize your experiments and chose viral vectors appropriate for your experiment.

Aug. 20, 2019, 12:31 p.m.

Find tips and best practices for producing lentivirus in your lab: from choosing a packaging system to optimizing transfection and improving titers.

Aug. 7, 2018, 12:31 p.m.

Learn how you can use planarian plasmids from the Sánchez lab to teach developmental biology techniques to new molecular biologists.

May 24, 2018, 1:20 p.m.

Learn how and why AAV vectors are being used in cell and gene therapies.

Nov. 7, 2017, 1:59 p.m.