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We narrowed to 782 results for: ina

Showing: 321 - 340 of 782 results

  1. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...post was originally written by Tyler Ford in March 2017 and updated by Angela Holmes and Christina Mork in..., spectinomycin, streptomycin, tetracycline, combinations of the above, or other. High or Low Copy... with highly repetitive sequences (prone to recombination in bacteria) such as lentiviral, retroviral,...

  2. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ... proceeding to ultracentrifugation to avoid contaminating your transduction with the packaging cells. ...may be the result of plasmid integration via recombination during transfection, and does not necessarily...PMC84922. 5. Ugai, Hideyo, et al. "Stability of a recombinant adenoviral vector: optimization of conditions...., Michael J. Yezzi, and Barbara R. Young. "Recombination events during integration of transfected DNA...

  3. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...system. Most Cascade complexes are comprised of a combination of Cas5, Cas6, Cas7, Cas8, and Cas11. Type I-...a fisherman would reel in a big catch. It was originally suspected that Cas3 moved a couple of kilobases...understood but likely occurs in similar regions. This combination of chewing up one stand and cutting the template...mutations if the chromosome is repaired with all the original DNA. With Cas3, you don’t have to play these odds...

  4. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ... program is the right fit for you without the financial and logistical stress that accompanies traveling...faculty, students, and each other. Before COVID-19, financial and logistical obstacles could make touring potential...COVID-19? How are students funded? Are there any financial aspects that are confusing? During a traditional...interviews for a variety of reasons (logistical, financial, etc…) now have options to fully engage in the...

  5. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ...(the precise mechanism isn’t well understood). Finally, TOPO cloning is another good option to consider...integration sequences, relies on site-specific recombination reactions catalyzed by BP Clonase and LR Clonase... to the use of toxic ccdB to prevent any non-recombinant vectors from propagating. The ligation-independent...since one can pool the components and define their final order through sequence homology. Similarly, Golden...

  6. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    Published
    June 22, 2017, 2:30 p.m.
    ... interest from its production site to its final destination. As an example, most secreted proteins are...pathway to their subcellular or extracellular destination. At the end of their life cycle, proteins may...through the vesicles to the lysosome where they are finally degraded (2, 3). Once you've created your fusion...

  7. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...expressed, they predominantly fluoresce one color but slowly mature until they predominantly fluoresce a ...of protein fusions or expressed independently) originally fluoresce green or blue and, when they mature...hallmarks of ideal FP timers: 1. Ratiometric determination of expression time The ratio of mature to immature...

  8. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ...repair mechanisms that can be exploited to either inactivate a gene or introduce a desired genetic alteration...expression. Its catalytic domains can be mutated to inactivate the protein’s nucleolytic capability, and this...remains intact while its nucleolytic activity is eliminated (Fig. 1) (1, 3). The gRNA can be further modified...tight control over Cas9 nuclease activity while eliminating the reliance on orthogonal Cas9 species. Within...

  9. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ... lab page, then onto the publication page, and finally on down to the individual plasmid page. The depositing...modifications listed can refer to changes made to the original backbone by the depositing lab, or by a previous...previously deposited with Addgene, you can list the original vector’s plasmid number here. Gene/insert: This...plasmid contains certain fluorescent proteins. Finally, for many plasmids you can find additional resources...

  10. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    Published
    Sept. 23, 2014, 3:05 p.m.
    ...last month it’s been viewed by users in the US, China, India, Canada, the UK, South Korea, and beyond!...protocols So that’s great, but what if someone in China improved upon that protocol? How would the thousands...prior to publication, and this will delay the dissemination of your improved protocol. Many scientists, ...need for quicker, more effective updating and dissemination of experimental protocols, simultaneously allowing...

  11. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ... nuclease domain. These mutations result in an inactive RNA-guided DNA binding protein (dCas9). dCas9 ...the next 3 ranked sgRNAs. Dolcetto was able to discriminate between non-essential and essential genes similarly... an improved activator design that consists of inactive Cas9-VP64, a sgRNA with two MS2 RNA aptamers, ... active sgRNAs for CRISPR-Cas9–mediated gene inactivation." Nature biotechnology 32.12 (2014): 1262. PubMed...

  12. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...proteins of interest has occurred. Although the original Y2H systems utilized the yeast Gal4 activator,...for screening greater numbers, but the latter eliminates the step of identifying prey proteins after screening... the experiment to reduce the likelihood of indiscriminate reporter gene activation. Including a prey-... libraries can be screened using both N- and C-terminal fusions of these proteins. This way both ‘ends...

  13. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ... bit of Addgene history... As you may be aware, Melina Fan, her brother Ken Fan, and her husband Benjie... corn toss boards, Kan Jam, and Ladder Ball (I finally discovered my hidden talent in Ladder Ball... there...popularly- cookies). 3. Do something outside of your ordinary environment Team building events like Escape the...Veckerelli is the Administrative and Operations Coordinator at Addgene. In addition to her operational responsibilities...

  14. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    Published
    Feb. 18, 2020, 2:15 p.m.
    ...Microbe meeting, but it was my favorite because I finally felt like I had a network of people to talk to ..., Kat Milligan-Myhre, and Pragati Chengappa.  Financial and moral supporters Denise Akob, Sara Alexander...Heather Hendrickson, Jonathan Jacobs, Mo Kaze, Christina Kellogg, Heather Martinez, Pat Schloss, Tara Smith...McGovern, and Julie Wolf. Apologies to anyone I’ve inadvertently left off this list. Many thanks to our guest...

  15. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type
    Blog Post
    Published
    April 2, 2019, 1:44 p.m.
    ...this is where most of our conference room names originated from. Let’s take a look at some of the stories...known to play a role in neural development, sex determination, and anterior-posterior segmentation (Jennings... SB transposon system including those from the original publication, Ivics et al 1997. Spaghetti Squash...may think that a gene called "spaghetti squash" originated from the squash itself, but think again. Spaghetti...

  16. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...their best interest to do so, sure, but it also eliminates that feeling of floundering one can have when...also need to talk to Tony from legal, Diana in finance, and Wu in graphic design. You could dread these...career options are expanding. As someone who coordinates cross-team projects at Addgene, I find that my... that led you into science in the first place. Finally, if you still find the idea of not holding a pipette...

  17. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...but some applications have been challenging or inaccessible. The Ting lab recently made two deposits that... Figure 3: A) LOV-Turbo becomes active when illuminated with blue light, catalyzing the biotinylation...that had been trafficked from the “source” to “destination”. Images adapted from Lee and Cheah et al. 2023.... (2019). A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex ...

  18. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ... in directly controlling muscle activity and coordinating movement, several additional brain areas that...dark areas (protected) as opposed to brightly illuminated ones (risky). They also prefer to explore novel... (Howerton at al. 2014). Addgene has several recombinase-expressing ready-made AAV vectors which can be...scientists study such as sleep, food intake, mobility/coordination, learning, and memory. We’ll cover these in ...

  19. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    Published
    Aug. 13, 2019, 12:58 p.m.
    ...strain distribution centers in the US, Europe, and China. In addition, the Zebrafish Model Organism Database...screens. Microscopy-based lineage tracing can help illuminate how signaling pathways—such as those regulated...patterns of gene activity and cell divisions that coordinate development of the body plan (Farrell et al.,...transgenesis with Tol2 and application for Cre/lox recombination experiments." Methods in cell biology. Vol. ...

  20. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ... Originally published Jul 14, 2015 and last updated Sep 16, 2020 by Beth Kenkel.  CRISPR genome editing...catalytically dead dCas13b to the catalytic domain of RNA deaminase ADAR2. Constructs containing the ADAR2 truncation...orthologs works for some applications, it doesn’t eliminate the need to deliver larger cargo such as base ...Cytosine and adenine base editing of the brain, liver, retina, heart and skeletal muscle of mice via adeno-associated...
Showing: 321 - 340 of 782 results