... Viral Service AAV Recombinases Viral Service: Recombinases These AAV encode... site specific recombinases. Viral...encode site-specific recombinases, which can be used to control gene expression. Dre AAV ID Name Promoter...AAV.TBG.PI.Cre.rBGe TBG none 8 Wilson Light-Inducible Recombinases ID Name Promoter Fluorophore Serotype(s) PI ...

...- N-terminal Flag-HA for mammalian expression pNIC-CTHF - C-terminal TEV-His6... TEV - N-terminal Flag-TEV for bacterial expression pCS2FLAG - N or C-terminal ...pKH3 - N or C-terminal 3xHA tag for mammalian expression pHAHA - N-terminal double ... C-terminal 3xHA tag for yeast expression Myc Epitope Tag pKMyc - N-terminal Myc ... TEV - N-terminal His6-TEV tag for bacterial expression pET His6 - C-terminal ... N-terminal His6-GST-TEV for bacterial expression pDest-565 - N-terminal ...Gateway) pPS892 - N-terminal GST for yeast expression pDM#834 - C-terminal GST ...

...Cre-lox recombination principles, scientists have developed constructs to activate/inactivate genes when...respectively. Split Cre: Cre recombinase is split in half into N and C terminal Cre fragments (NCre and CCre...photo-activatable cre recombinase CAG AAV Bruchas 75268 pAAV-CRY2-CreN photo-activatable cre recombinase CAG AAV Bruchas...pCMV-Tag2B-NCre N-terminal split-Cre CMV Mammalian Hirrlinger 106369 pCMV-Tag3B-CCre C-terminal split-Cre CMV...pCMV-Tag2B-NCreERT2 N-terminal split-Cre-ERT2 CMV Mammalian Hirrlinger 106371 pCMV-Tag3B-ERT2CCre C-terminal split-Cre-ERT2...photo-activatable Cre recombinase CMV Mammalian Sato 122961 pcDNA3.1_PA-Cre-Y324F Catalytically inactive PA-Cre CMV...FRT: Alternative recombinase system for the control of gene expression. Flp recombinase recognizes FRT ...

...enhancer Viviana Gradinaru 99133-AAV1 pAAV-CAG-fDIO-mNeonGreen An AAV genome with Flp recombinase-dependent ... tools and emphasizes their rapid and broad dissemination to the research community. Browse plasmids ,...83894-AAV1 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent dTomato expression in forebrain GABA-ergic...83894-AAV9 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent dTomato expression in forebrain GABA-ergic...83894-AAVrg pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent dTomato expression in forebrain GABA-ergic... 83895-AAV1 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain GABA-ergic... 83895-AAV2 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain GABA-ergic...

...protein kinase kinase kinase 14 FTDCR1B, HS, HSNIK, NIK MAP3K8 mitogen-activated protein kinase kinase kinase...protein kinase kinase 1 MAPKK1, MEK1, MKK1, PRKMK1 MAP2K2 mitogen-activated protein kinase kinase 2 FLJ26075...Kits and Collections Human Kinase ORF Kit : 558 distinct human kinases and kinase-related protein Open Reading...expression. Myristoylated Kinase Kit : Consists of 190 human kinases and kinase-related open reading frames...phosphoinositide-3-kinase, catalytic, delta polypeptide p110D PIK3CG phosphoinositide-3-kinase, catalytic, gamma...phosphoinositide-3-kinase, regulatory subunit 1 (alpha) GRB1, p85, p85-ALPHA PIK3R2 phosphoinositide-3-kinase, regulatory...plasminogen activator, urokinase ATF, UPA, URK, u-PA PLAUR plasminogen activator, urokinase receptor CD87, UPAR...

...dual-transgenic recombinase-expressing mouse lines 2-9 and combinations of transgenic recombinase-expressing...2014. INTRSECT INTRSECT (intronic recombinase sites enabling combinatorial targeting) is a synthetic molecular... Cre and Flp (B,E). When the correct combinations of recombinases are present, the introns are excised...expression plasmids that combine multiplexed recombinases, including Cre, Flp, and VCre to enable viral...Intersectional expression approaches combine multiplexed recombinases, including Cre, Flp, and VCre to enable viral...intersectional cell population definitions using recombinases. A) Cre and Flp are multiplexed to enable intersectional...expressed in cells based on a doubly-specified combination of genetic and/or anatomical-defined parameters...

...inducible vector for expression of a recombinant protein with an N-terminal 6x His tag and an MBP tag. Compatible...inducible vector for expression of a recombinant protein with an N-terminal 6x His tag and an MBP tag. Contains...promoters (Lactose/IPTG inducible) Various combinations of N and C-terminal Strep and His tags Thorben Dammeyer... and without different combinations of His and Strep tags as well as N-terminal PelB tags for localization...be expressed in B. subtilis Various combinations of N and C-terminal Strep, His, MBP, TRX, GST, SUMO, MISTIC...Gang Bao Gateway cloning and expression of an N-terminally His-tagged TALEN fused to an E. coli codon optimized...Gang Bao Gateway cloning and expression of a C-terminally His-tagged TALEN fused to an E. coli codon optimized...

...Mitogen-activated protein kinase kinase MET MET proto-oncogene, receptor tyrosine kinase MLST8 MTOR associated...STK4 Serine/threonine kinase STK11 Serine/threonine kinase 11 TBK1 TANK binding kinase 1 TFDP TFDP1 TFDP2... CDK6 Cyclin-dependent kinase CNKSR CNKSR1 CNKSR2 Connector enhancer of kinase suppressor of Ras CYTH2...receptor tyrosine kinase 2 ERK MAPK1 MAPK3 Also known as MAPK; Mitogen-activated protein kinase ETS ETS1 ETS2...coiled-coil containing protein kinase ROS1 ROS proto-oncogene 1, receptor tyrosine kinase RPS6KA RPS6KA1 RPS6KA2... Ribosomal protein S6 kinase RPS6KB RPS6KB1 RPS6KB2 Ribosomal protein S6 kinase B1 RPTOR Regulatory associated...within the cell. GTPases have an active (GTP) and inactive (GDP) state, regulated by the addition and removal...

...Flpo recombinase Recombinases Zhang 121675 pAAV-EF1a-fDIO-Cre EF1a Flp-dependent Cre recombinase Recombinases...EYFP Control Gradinaru 105547 pENN.AAV.EF1a.eGFP.WPRE.rBG EF1a EGFP Control Wilson Recombinases 24593 AAV-pgk-Cre...expression Recombinases Larsen 105553 pENN.AAV.hSyn.Cre.WPRE.hGH Syn Cre expression Recombinases Wilson 107788...mCherry expression Recombinases Deisseroth 55636 pAAV-EF1a-Cre EF1a Cre expression Recombinases Deisseroth 55637... expression Recombinases Deisseroth 55638 pAAV-EF1a-vCre EF1a VCre expression Recombinases Deisseroth ...expression Recombinases Zeng 51669 AAV phSyn1(S)-FlpO-bGHpA Syn Flpo expression Recombinases Zeng 183412...possible) in vivo testing, and full sequencing of the final viral vector preparation. In some cases, in vivo...

...viruses (AAV) are small viruses originally discovered as contaminants of adenovirus stocks. One major ...amplification system Gradinaru 129024 pProvector_AAV2_586_PLGLAR AAV2_586_PLGLAR Recombinant AAV2 rep/cap plasmid...low immune response and low toxicity. Although recombinant AAV does not integrate into the host genome, ...Cap controlled by tTA-TRE amplification system Gradinaru 103006 pUCmini-iCAP-PHP.S PHP.S AAV packaging ...Cap controlled by tTA-TRE amplification system Gradinaru 127847 pUCmini-iCAP-PHP.V1 PHP.V1 AAV packaging...Cap controlled by tTA-TRE amplification system Gradinaru 175004 pUCmini-iCAP-AAV.CAP-B10 B10 AAV packaging...Cap controlled by tTA-TRE amplification system Gradinaru 175005 pUCmini-iCAP-AAV.CAP-B22 B22 AAV packaging...

...displays enhanced transduction of the retina following subretinal administration and enhanced transduction...transduction and lateral spread in the retina following subretinal injection. It was identified by John ...transduction and lateral spread in the retina following subretinal injection as compared to the parental...Heparan Sulfate Binding on Transduction of Retina by Recombinant Adeno-Associated Virus Vectors. J Virol ...may also like: Viral Service: All AAV Viviana Gradinaru PHP AAV Serotypes Blog: Viral Vectors As part ...possible) in vivo testing, and full sequencing of the final viral vector preparation. These viral vector preparations...delivered directly to the brain parenchyma or subretinal space Serotypes The following serotypes were ...

...sequence in the 3' UTR Carl Novina 33154 pAC-Luc Firefly Creation of N-terminal Firefly fusions in Drosopholia...luciferase reporter. These plasmids improve over the original STARR-seq plasmids by using the bacterial ORI ...which was a source of false positives with the original STARR-seq method. Learn more about STARR-seq in...87067 pcDNA3.1-ccdB-Nanoluc NanoLuc® Creation of N-terminal Nanoluc fusions using Gateway cloning Mikko Taipale... pLenti6.2-ccdB-Nanoluc NanoLuc® Creation of C-terminal Nanoluc fusions using Gateway cloning. Lentival... pLenti6.2-Nanoluc-ccdB NanoLuc® Creation of N-terminal Nanoluc fusions using Gateway cloning. Lentiviral...87070 pcDNA3.1-Nanoluc-ccdB NanoLuc® Creation of N-terminal Nanoluc fusions using Gateway cloning Mikko Taipale...

...luciferase-2A-Cre recombinase 60226 : sgRNA cloning backbone with luciferase-2A-Cre recombinase Vectors for ... to NeuN, plus Cre recombinase 60228 : sgRNA targeted to LacZ, plus Cre recombinase 60229 : sgRNA cloning... backbone with Cre recombinase 60230 : sgRNA targeted to NeuN, plus Cre recombinase-2A-EGFP-KASH 60231...plasmids. CRISPR loci in microbial hosts contain a combination of CRISPR-associated (Cas) genes as well as non-coding...consists of three components: A nucleolytically inactive Cas9-VP64 fusion A sgRNA incorporating two MS2...targeting efficiency. These plasmids use the inverted terminal repeats (ITR) from AAV serotype 2. SaCas9 only...KRAS , p53 , and LKB1 , plus luciferase-2A-Cre recombinase, and Kras G12D HDR template 60225 : control for...

...pCAG-T7-TALEN(Sangamo)-Destination Pawel Pelczar pCAG-T7-TALEN(Sangamo)-Destination constructs were designed...TALEN(Sangamo)-FokI-KKR-Destination 40132 pCAG-T7-TALEN(Sangamo)-FokI-ELD-Destination Golden Gate TALEN 2.0...new destination vectors can be used to create TALE-transcription factors and are used at the final assembly...pC-GoldyTALEN and RCIsricpt-GoldyTALEN are designed as destination vectors for the Voytas lab Golden Gate TALEN ...-BB and pTAL6-BB are designed as alternative destination vectors for the Golden Gate TALEN kit (Bogdanove...expression vector resulting in shorter N- and C-terminal tal protein segments (136AA and 63AA, respectfully...several later studies. pCAG-T7-TALEN(Sangamo)-Destination vectors are available with homodimeric or enhanced...

...pAAV-mDlx-NLS-mRuby2 Dlx mRuby2 Control Gradinaru 104055 pAAV-CAG-eYFP CAG EYFP Control Gradinaru 104061 CAG-NLS-GFP CAG...light-inducible recombinase iCreV Zeng 140136 pAAV-EF1a-iDreV EF1a light-inducible recombinase iDreV Zeng ... Engineered systemic constructs from Viviana Gradinaru's lab at Caltech. Viral...engineered systemic serotypes from the Viviana Gradinaru laboratory and the Caltech CLOVER Center . As ...possible) in vivo testing, and full sequencing of the final viral vector preparation. Systemic Delivery AAV ...NHP, non-human primate. Image courtesy of the Gradinaru Lab and the Caltech CLOVER Center and was adapted...CAG NLS-GFP Control Gradinaru 105530 pAAV.CMV.PI.EGFP.WPRE.bGH CMV EGFP Control Wilson 105547 pENN.AAV....

...nickase or catalytically inactive “dead” Cas9 (dCas9) to a cytidine deaminase like APOBEC. Base editors...target sequence present in the gRNA. CRISPR was originally employed to knock out target genes in various... gRNA binding that shifts the molecule from an inactive, non-DNA binding conformation into an active DNA-binding... that increase Cas9 proofreading and target discrimination. All three high fidelity enzymes generate less...resulting population of cells will contain some combination of wild type alleles, NHEJ-repaired alleles, ...creating an A to G (or T to C) change. Adenine DNA deaminases do not exist in nature, but have been created... of the Escherichia coli TadA, a tRNA adenine deaminase. Like cytosine base editors, the evolved TadA...

...the N-terminal portion of split mCherry, while a phosphobinding domain is fused to the C-terminal mCherry... of kinases of interest by mass spectrometry in a technique called serine-oriented library/kinase-library...overall improved ease of use when expressing recombinant phosphoproteins. These attributes make rEcoli...111705-8 ) is available from Addgene. Screening kinases. The mode #1 phosphosite library can also be synthesized...enhanced biological tolerance. Mohler K, Moen JM, Rogulina S, Rinehart J. Mol Syst Biol 2023 Aug 8;19(8):...interactions. Barber KW, Muir P, Szeligowski RV, Rogulina S, Gerstein M, Sampson JR, Isaacs FJ, Rinehart... Nat Biotechnol. 2018 Aug;36(7):638-644. PubMed Kinase Substrate Profiling Using a Proteome-wide Serine-Oriented...

...absence of unexpected DNA contaminants. The data is also used to determine recombination rates in FLEX/DIO/fDIO...Endotoxin contamination in vector preparations can alter the immunogenic properties of the final product...mycoplasma contamination. To date, Addgene has never had a case of mycoplasma contamination. In the event...is performed on viral genomes isolated from the final AAV preparation. Sequencing results are analyzed...particularly in large animal studies. Endotoxin contamination is minimized by using an endotoxin-free plasmid.... To minimize the immunogenic properties of the final vector preparation, the quantity of gram-negative...Takara, and is routinely tested for mycoplasma contamination using mycoplasma detection kits. The cell line...

...Verdoes Backbones for mouse recombinant antibodies A panel of recombinant monoclonal antibodies against... Collection Addgene distributes ready-to-use recombinant antibodies made from plasmids in our collection...alternative approach is to use synthetically produced recombinant antibodies, created by cloning antibody components...Some scientists choose to work with full length recombinant monoclonal antibodies (R-mAbs) expressed in mammalian...research applications from the NeuroMab/Trimmer Lab Recombinant mAb Collection . Once the plasmids are transiently...of production due to their small size. These recombinant antibody fragments can be used in experiments...tool kit for rapid cloning and expression of recombinant antibodies. Sci Rep. 2014 Jul 30;4:5885. Andrew...

...growth and proliferation. mTOR, a serine/threonine kinase, is found in two complexes, mTORC1 and mTORC2... and multiple familial cancers are caused by inactivating mutations in negative regulators of mTORC1. ...homolog LKB1 Also known as STK11; serine/threonine kinase 11 mTOR Mechanistic target of rapamycin NF1 Neurofibromin...S6K Also known as RPS6KB1; Ribosomal protein S6 kinase B1 TSC1/2 TSC1 TSC2 Tuberous sclerosis VHL Von ...LST8 homolog GSK3 GSK3A GSK3B Glycogen synthase kinase 3 MDM2 MDM2 proto-oncogene mSin1 Also known as ...as MAPKAP1; Mitogen-activated protein kinase associated protein 1 mTOR Mechanistic target of rapamycin ...; tumor protein p53 PDK1 Pyruvate dehydrogenase kinase, isozyme 1 PI3K Catalytic Subunits PIK3CA PIK3CB...