We narrowed to 364 results for: crispr
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  TypeBlog PostPublishedJuly 27, 2023, 1:15 p.m....Vectors for Delivery of Gene-Editing Systems Based on CRISPR/Cas: Current State and Perspectives. Viruses, 13...
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  Tips for arabidopsis transformationTypeBlog PostPublishedOct. 25, 2018, 1:23 p.m....Addgene blog Engineering the plant genome using CRISPR/Cas9 Using pSiM24 for simplifying plant genetic...
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  Tips for a 1st time AAV user (by a Rookie AAV user)TypeBlog PostPublishedOct. 23, 2018, 11:49 a.m....packaging transfections Find tips for a first time CRISPR user Resources on Addgene.org Learn how Addgene...
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  The Challenges of Cell CultureTypeBlog PostPublishedMay 5, 2016, 2:30 p.m....Blog Learn All about Mammalian Vectors Read up on CRISPR Delivery Systems for Mammalian Cells Learn about...
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  Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPsTypeBlog PostPublishedOct. 27, 2015, 3 p.m.... genes for proteins in these compartments using CRISPR systems should strongly consider using moxFPs over...
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  Plasmids 101: Control PlasmidsTypeBlog PostPublishedApril 9, 2015, 3:29 p.m....or genome editing technologies, such as RNAi or CRISPR, non-targeting controls may be appropriate as they...
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  Getting the Most from Your Lentiviral TransductionTypeBlog PostPublishedApril 7, 2016, 2:30 p.m....Elements Learn How to Do Genome-Wide Screens with CRISPR Lentiviral Vectors Additional Resources on Addgene.org...
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  Five Popular Model OrganismsTypeBlog PostPublishedApril 11, 2019, 12:39 p.m.... Mice can be easily manipulated with tools like CRISPR to make transgenic lines. The fruit fly (Drosophila...
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  Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right ConnectionTypeBlog PostPublishedApril 9, 2019, 1:13 p.m....revolution in genome editing methods (most notably CRISPR/Cas mediated HDR) enables the tagging of endogenous...
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  Plasmids 101: BiotinylationTypeBlog PostPublishedNov. 15, 2018, 1:50 p.m.... interactions Learn how biotin can be used with CRISPR Additional resources ThermoFisher Scientific...
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  Fluorescent Proteins 101: When GFP lets you downTypeBlog PostPublishedAug. 23, 2018, 12:05 p.m....genomic loci in living human cells by an optimized CRISPR/Cas system." Cell 155.7 (2013): 1479-1491. PubMed...
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  Evolution of Lab TechniquesTypeBlog PostPublishedJune 21, 2016, 2:30 p.m.... instance, the popular genome-engineering tool, CRISPR, may one day make techniques used to generate transgenic...
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  Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of NeuronsTypeBlog PostPublishedApril 24, 2015, 2:39 p.m....CRISPR-Cas9 genome editing may be the hot new way to manipulate gene expression, but other gene manipulation...
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  Hot Plasmids: Summer 2024TypeBlog PostPublishedAug. 27, 2024, 1:15 p.m....Genome-wide programmable transcriptional memory by CRISPR-based epigenome editing. Cell, 184(9), 2503–2519... Gilbert Labs have previously deposited their CRISPRoff system — a programmable epigenetic editor consisting...deposited with Addgene. These variants, including a “miniCRISPR” version, are much smaller proteins than SpyCas9...
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  Technologies Enabled by NanoLuc® LuciferaseTypeBlog PostPublishedFeb. 8, 2018, 12:17 p.m....detect endogenous tagged genes generated through CRISPR/Cas9 knock-in. Finally, NLuc is very inviting for...
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  Quick Guide to All Things LentivirusTypeBlog PostPublishedMarch 21, 2017, 2:30 p.m....vectors fantastic options for delivering shRNA, CRISPR/Cas9 components, and fluorescent sensors. Retroviral...
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  Viral Vectors 101: Optogenetic ToolsTypeBlog PostPublishedJune 27, 2023, 1:15 p.m....electrical activity to gene expression, and even CRISPR based gene editing. But, how do you decide which...
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  Lambda Red: A Homologous Recombination-based Technique for Genetic EngineeringTypeBlog PostPublishedDec. 15, 2016, 3:57 p.m....Knockin Plasmids to Disrupt Genes in Mammalian Cells CRISPR Methods for Bacterial Genome Engineering Resources...
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  Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent ProteinsTypeBlog PostPublishedAug. 15, 2017, 1:24 p.m.... of other technologies such as optogenetics and CRISPR/Cas9 will help immunologists create even better...
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  How to Deposit Your Plasmids with AddgeneTypeBlog PostPublishedFeb. 27, 2024, 2:15 p.m....sequences cloned into it. gRNAs are used for directing CRISPR/Cas9 to a targeted genomic location, while shRNAs...