We narrowed to 376 results for: crispr

Controls are required for any plasmid-based experiment in order to accurately assess the results. This blog posts reviews different types of control plasmids, including but not limited to internal controls, negative controls, positive controls, and replicate controls.

April 9, 2015, 3:29 p.m.

Model organisms are species that are widely studied because they are easy to maintain and modify in the lab. Common model organisms include the mouse, the fruit fly, yeast, zebrafish, and the worm.

April 11, 2019, 12:39 p.m.

Learn about some things to consider when designing your fusions - where to make the fusions, the size and shape of the protein, and linker size.

April 9, 2019, 1:13 p.m.

Learn how biotin is used in a variety of molecular biology experiments and makes it easier for researchers to label and purify macromolecules of interest.

Nov. 15, 2018, 1:50 p.m.

Learn how various lab techniques have evolved but still remain relevant in many research arenas today.

June 21, 2016, 2:30 p.m.

An overview of the Brainbow system and its variants and how they utilize Cre-lox for multicolor labeling of neurons.

April 24, 2015, 2:39 p.m.

Check out some of the new and useful tools recently deposited in the Addgene repository!

Aug. 27, 2024, 1:15 p.m.

GFP is the most widely used fluorescent protein, but there are several instances that limits its use. GFP requires oxygen, is sensitive to acid, and its large size can be problematic. When thinking about ways to label proteins, a different probe may be more appropriate.

Aug. 23, 2018, 12:05 p.m.

Learn about the many fluorescent protein tools enabled by the nanoLuc luciferase.

Feb. 8, 2018, 12:17 p.m.

History, composition, and tips for lentiviral vectors all in one convenient blog post.

March 21, 2017, 2:30 p.m.

Lambda Red mediated recombineering can be used to generate point mutations in plasmids and bacterial genomes. Learn how to use Lambda Red in this post.

Dec. 15, 2016, 3:57 p.m.

Learn about fluorescent protein techniques you can use to monitor cellular trafficking throughout an organism.

Aug. 15, 2017, 1:24 p.m.

Need help with the plasmid deposit process at Addgene? Consult this post for an in-depth walk through the entire process.

Feb. 27, 2024, 2:15 p.m.

If you can't find a validated antibody for your flow experiment, this blog post can help you validate your antibodies yourself.

Sept. 10, 2024, 1:15 p.m.

In this episode of the Addgene Podcast, learn all about Joachim Goedart and his work with fluorescent proteins at the University of Amsterdam.

March 9, 2018, 2 p.m.

Curious about systemic capsids? Looking for one to test in your experimental system? This 101 is an introduction to systemic capsids uses and types!

Oct. 12, 2023, 1:15 p.m.