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We narrowed to 25 results for: loxP

Showing: 11 - 20 of 25 results
  1. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    Published
    May 12, 2015, 1:32 p.m.
    ...Cre may induce recombination at pseudo- or cryptic loxP sites (estimated to occur at a frequency of 1.2 ...458-65. doi:10.1007/s004270100167. PubMed. Cryptic loxP sites in mammalian genomes: genome-wide distribution...
  2. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    Published
    July 7, 2015, 3:36 p.m.
    ...easy to remove after injection, they added flanking loxP sites and a heat-shock inducible Cre to generate...schematic below.) After self-excision, the resulting loxP scar is located in this synthetic intron, and only...
  3. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...technology (Cre/loxP) to remove the donor cassette. While this method can be effective, a loxP site is left...Grégoire and M. Kmita, “Recombination between inverted loxP sites is cytotoxic for proliferating cells and provides...
  4. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...series of primary cell types using Cre and various loxP reporters. In both mouse and human embryonic stem...
  5. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...transgenes that are flanked by loxP sites. While there is only a small loxP scar behind at the site of excision...
Showing: 11 - 20 of 25 results