...promoters, including ubiquitous ones such as EF-1α and CAG. Figure 2: Dual lentivirus system with ubiquitous...

... version is commonly used in promoter hybrids. CAG General expression mRNA Strong hybrid mammalian promoter...

...overlapping 5′ and 3′ EGFP fragments under the ubiquitous CAG promoter. The approximately 500 bp genomic fragment...cassette. (b) pCAG-EGxxFP target plasmid and pX330-sgRNA plasmid (Mashiko et al. 2013). The pCAG-EGxxFP target...cloned directionally into the BbsI site. (c) The pCAG-EGxxFP target plasmid was co-transfected with pX330...Once amplified, you can insert this region into a pCAG-EGXXFP plasmid using standard cloning techniques...of transfecting HEK293T cells with your modified pCAG-EGXXFP plasmid along with the individual gRNAs in...Remember the primers you designed to generate your pCAG-EGXXFP plasmid? They are the perfect primer sets...

...different cells. Promoters such as chicken-beta-actin or CAG can drive strong early gene expression, but can become...

...thoroughly tested in your cells.  Some promoters, such as CAG are very strong and may require less viral particles...

...integration is TA dinucleotides and it leaves behind the CAG DNA footprint from its terminal sequences at the ...

...that AAV-PHP.eB, when paired with the ubiquitous CAG promoter, is relatively biased toward L5 and inhibitory...is quite pronounced, even when using a ubiquitous CAG promoter. Spatial transcriptomics shows that its ...

...Autobow systems are available with either the Thy1 or CAG promoter, while Brainbow AAV is under control of ...target sequence in between two fragments of EGFP in pCAG-EGxxFP. The EGFP fragments contain 482bp of overlapping...

...mice. The Jacks Lab used this backbone to target a CAG-driven loxP-stop-loxP cassette into the Rosa26 locus...pCS2TAL3-RR Pawel Pelczar pCAG-T7-TALEN(Sangamo)-Destination series, pCAG-Golden-Gate-Esp3I-Destination...pCAG-Golden-Gate-Esp3I-Destination Takashi Yamamoto pcDNA-TAL-NC2, pCAGGS-TAL-NC2 Charles Gersbach pcDNA3.1-GoldenGate, pcDNA3.1...

...oligonucleotides Cas9, photocaged-gRNA Gene editing Manna et al., 2019   NPPOC-caged trimethoprim (TMP) ...transcription Zhou et al., 2020   NPOM-caged nucleobases Cas9, photocaged-gRNA Gene editing For more details...chromatin, and study kinetics. Using chemistry to photocage CRISPRs The aforementioned techniques each employed...These researchers used a genetically encoded photocaging technique to insert a light-removable protecting...protecting group, specifically a nitrobenzyl photocaged lysine (PCK), on the Cas9 protein (Hemphill et al.,..., read this article). The group first tested photocaging various lysines in Cas9 to determine which best... ability to cleave targeted DNA, settling on photocaging the K866 lysine, as seen in the figure below....

...final Forward Primer sequence of 5'-TAAGCAGAATTCATGTGGCATATCTCGAAGTAC-3'. For the Reverse Primer, the design...final Reverse Primer sequence of 5’-TGCTTAGCGGCCGCTCAGTACTTCGAGATATGCCA-3’. Preparing the PCR product for...

...if an experimenter picks up a mouse from the home cage and places it into the experimental setup, the mouse...individually versus in groups), health status, diet, cage enrichment, stress level, origin (vendor or in-house...

...adaptations of this method have been to tag, cage, uncage, and trap the chemical fluor with the genetically...

...BinningSingletons mixer with new friends @iprophage @robincagle5 pic.twitter.com/52KQDMeSjO — Brianna Weiss (@... even more valuable program to Microbe 2020 in Chicago.  Finding our place in networking and mentoring...

...localization sequence) and expressing it from a CAGGs promoter. However FLPe is still less efficient than...binding domain (LDB) to the C-terminus of FLP or Cre (pCAG-CreERT2 #14797). These Cre/ FLP versions are retained...

...enzymatic activity. In addition, proteins can be “caged” by fusion to specific domains thereby rendering... The authors use PhoCl in combination with this caging technique to beautifully demonstrate light-dependent...

...are some of our 2017 contest winners:   Chicagoland's Community Biology Lab (@ChiTownBio) and one ...

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...Marathon for the MGH Pediatric Oncology and the Chicago Marathon to benefit Neurofibromatosis, New England...

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