We narrowed to 251 results for: cas9

Discover the tools you need to perform plant genome engineering with CRISPR.

Oct. 11, 2016, 2:30 p.m.

Learn how to use Cas9 nickases for genome editing, including homology directed repair.

March 15, 2018, 12:59 p.m.

The development of adenoviral vectors for the delivery of CRISPR/Cas9 expands genome engineering to primary cells.

Dec. 10, 2020, 1 p.m.

Did you know CRISPR can be used to target non-coding RNAs? In this blog post, we explain the pros and cons of different approaches.

Nov. 14, 2024, 2:15 p.m.

Learn about the csRNP epitope tagging method - a new and efficient way to tag endogenous genes using CRISPR.

June 28, 2018, 3:01 p.m.

Learn all about plasmids for protein degradation, endosome biosensing, studying the epigenome, and controlling gene expression in yeast.

April 23, 2018, 2 p.m.

Protospacer Adjacent Motifs (PAM) serve as a binding signal for Cas proteins. Here we highlight the many Cas proteins and their PAM sequence variations.

Aug. 20, 2020, 2:30 p.m.

Learn about new plasmids including optogenetic control of synaptic transmission, acoustic reporter genes, miRNA reporter vectors, and CRISPR-Cas9 delivery for in vivo screening.

June 6, 2019, 12:41 p.m.

Learn about xCas9, an engineering Cas9 variant with broad PAM flexibility and high on-target specificity.

March 28, 2018, 6:52 p.m.

Take behind-the-scenes look at the isolation of a thermostable Cas9, IgnaviCas9, from Yellowstone and learn about the advantages of using a thermostable Cas9 protein.

Nov. 12, 2019, 2 p.m.

Choosing a CRISPR activator? This article features four of the most popular CRISPR activators (dCas9-VP64, SAM, SunTag, and VPR) and some newer activators like dCas9-CBP and SPH.

Oct. 6, 2020, 1:15 p.m.

An easy demethylation protocol uses CRISPR-based dCas9-directed DNA avoids the use of Tet

Dec. 14, 2021, 2:15 p.m.

Discover the best situations to use Cas9 activators in your experiments with tips from their creators.

Aug. 18, 2016, 2:30 p.m.

This post highlights ways to overcome the AAV size limitation to deliver CRISPR editing tools to cells.

Sept. 16, 2020, 1 p.m.

New to using CRISPR pooled libraries for your screen? Here, we cover how to choose a CRISPR library, and the steps and tips for a successful CRISPR screen.

Aug. 18, 2015, 2:30 p.m.

Guest blogger Pooran Dewari presents results of an NgAgo survey he gave to some of the first NgAgo users. Find out how NgAgo compares to Cas9 so far.

Aug. 4, 2016, 2:30 p.m.

Researchers at the Wyss Institute for Biologically Inspired Engineering explain how you can use truncated gRNAs to regulate gene expression.

Jan. 10, 2017, 3:37 p.m.

Various lentiviral CRISPR-Cas9 libraries have been developed to perform genome-wide knockout screens, including the GeCKO library from Feng Zhang's lab.

Feb. 20, 2014, 4:57 p.m.

Use anti-CRISPR proteins to turn off genome editing and prevent off-target effects. In this article, read about how it works and its applications

July 23, 2020, 1:20 p.m.

Learn about some newly developed Cas proteins and how to choose a Cas protein based on site accessibility, specificity, and sensitivity.

Nov. 5, 2019, 1:28 p.m.