We narrowed to 37 results for: gateway

Learn about different strategies used to generate plasmids for multiplex gRNA expression and how to use them in various organisms.

Sept. 10, 2020, 11:45 a.m.

Learn how to use and apply NEBuilder HiFi DNA Assembly Technology from New England Biolabs for quick and accurate plasmid cloning.

Sept. 6, 2017, 1:01 p.m.

Learn how you can use the Broad Target Accelerator Plasmid Collections from to help enable precision cancer medicine research.

May 11, 2017, 2:30 p.m.

Blue-white screening is a well loved technique that allows scientists to quickly identify recombinant clones for further analysis.

June 4, 2015, 1:03 p.m.

Check out all of the plasmids featured in our hot plasmids articles from 2016.

Dec. 22, 2016, 3:03 p.m.

Learn about click biology and how you can use it to advance your experiments and explore biological phenomenon.

Oct. 21, 2025, 1:15 p.m.

Sequence and ligation independent cloning (SLIC) lacks the strict requirements of many standard cloning techniques. Learn how to use SLIC in this post.

Sept. 26, 2024, 1:15 p.m.

Learn about the various expression systems and plasmids you can use to produce your protein of interest.

June 7, 2018, 1:17 p.m.

An overview of split fluorescent proteins and a highlight of some of the most popular and newest ones available on Addgene.

May 3, 2022, 1:15 p.m.

Read updates on new developments in cancer research and learn about Addgene's Cancer Collection pages.

Sept. 6, 2016, 2:30 p.m.

Learn about the components of a plasmid page on our website and shows how the different components can be useful to you

Feb. 4, 2016, 3:30 p.m.

The yeast two hybrid (Y2H) assay is useful for probing interactions between proteins. Learn more about the Y2H assay and how you can use it in this post.

Oct. 22, 2015, 2:30 p.m.

The basics of Fluorescence Resonance Energy Transfer (FRET), including tips and parameters to consider when using FRET for your experiments.

Nov. 5, 2014, 3:41 p.m.

Get the basics on RNA interference (RNAi), how it compares with CRISPR, and tools for RNAi studies in plants.

Oct. 27, 2020, 1:15 p.m.

Learn how to target donor and acceptor fluorophores for FRET experiments.

July 19, 2016, 2:30 p.m.

Need help with the plasmid deposit process at Addgene? Consult this post for an in-depth walk through the entire process.

Feb. 27, 2024, 2:15 p.m.

If you can't find a validated antibody for your flow experiment, this blog post can help you validate your antibodies yourself.

Sept. 10, 2024, 1:15 p.m.