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We narrowed to 782 results for: ina

Showing: 401 - 420 of 782 results

  1. Hot Plasmids - May 2022

    Type
    Blog Post
    Published
    May 26, 2022, 1:15 p.m.
    ...method (M-CREATE) by: Brian O'Neill  The Viviana Gradinaru Lab has previously developed the multiplexed version...mutations. These plasmids contain catalytically inactive dCas9 and microbial SSAPs (dCas9-SSAP) which insert...specific transcription response element (TRE) or kinase translocation reporters (KTRs). Find visual barcode...

  2. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...Did you catch our April AAV webinar with Tim Miles, PhD, Director of the CLOVER Center at CalTech? If...are sold in packages of 10…)  If you missed the webinar, you can watch the presentation and Q&A session...recent review.      And that's a wrap on our AAV webinar! Special thanks to our panel of experts, Tim Miles...

  4. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    Published
    Nov. 24, 2020, 2:15 p.m.
    ...log off for the day. 1:45 PM--Write final report. Work on a final report for a user research project I...help implement solutions for the stakeholders. Finally, software product managers test the solutions with...

  5. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    Published
    July 28, 2017, 1:30 p.m.
    ... by geneticists to study gene function. In this final installment, I’ll provide a brief introduction as...transgenes in different loci allows for genetic recombination so that the transgenes can be inherited on the...Engineering Posts Use FLEx Vectors for Site-Specific Recombination Resources at Addgene.org Browse Drosophila...

  6. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ...since it is impossible to check all possible combinations of biology and equipment. Because the performance...(Goedhart et al, 2011) allows for a precise determination of brightness in cells, under realistic experimental...fluorescent protein in the host cell, which is a key determinant of the practical brightness. Therefore, practical...

  7. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...s ability to process its own pre-crRNA arrays eliminates the need to include multiple promoters to drive...processing, i.e. tRNAs or Csy4 cleavage sites. This eliminates bulk from crRNA expression plasmids resulting...crRNA array (bolded red arrow).  This undoes the original reversing of the direct repeats, leading to expression...

  8. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ... approach, and conclusions (see above). Keep original files in a separate folder: You will often use...for a single use, save the script as originalName_descriptionOfEdit in the same directory where it was ...that was temporarily used. Just be sure that the original code remains in tact! Take notes Comment in ...

  9. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...insert targeted mutations through homologous recombination. To further increase the utility of CRISPR/Cas9...containing the RNA pol III promoter, gRNA and pol III terminator. These relatively large cassettes (considering...Size is also a factor; the pol lll promoter and terminator need be encoded only once, and each <200 bp tRNA-gRNA...

  10. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...This post was originally written by Jennifer Tsang and updated by Rachel Leeson. Have you ever wondered...cloning, waiting, and repeating before you can finally dive deep into generating the data you’re excited...cost $155.25 in reagents and consumables today. Finally, this doesn’t account for the most valuable component...

  12. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ... deletions.   Cas12a – The staggered cutter  Originally classified as Cpf1, Cas12’s primary difference...Another similarity to Cas12a is that Cas14 also indiscriminately cleaves non-complementary ssDNA when engaged... the genome. Cas13, similar to Cas14, also indiscriminately cleaves ssRNA when engaged at its RNA target...

  13. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...or awaiting a final publication in a journal.  Preprints can be one tool in a combination of strategies... Addgene ID for their deposited plasmids in the final journal publication or an updated preprint, further...

  14. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...ligation assay (PLA). The clever combination of FUNCAT and PLA PLA was originally published by the Söderberg...16590564. PubMed Central PMCID: PMC222790. 6. Buxbaum, Adina R., Bin Wu, and Robert H. Singer. "Single β-actin...

  15. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...questions the conclusion of inter-homologue recombination was released. This blog has not been updated...al. were interested in correcting an autosomal dominant mutation in MYBPC3 that leads to hypertrophic ...embryos to find those that do not carry a given dominant disease-causing gene, or that carry only one copy...

  16. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ...time). Other illumination strategies, such as 2-photon-excitation, selective plane illumination, TIRF or ...experiments on a single day on a stable system. A final piece of advice is to measure the excitation power...

  17. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...yellow, or magenta, depending on the hairpin combination bringing the total number of individual colors...Adding an additional hairpin/fluorescent protein combination, for example, with a far-red FP, would increase...15! They also envision using CRISPRainbow in combination with gene editing. CRISPRainbow requires very...

  18. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    Published
    Jan. 11, 2017, 3:30 p.m.
    ...available (below). However, on November 22nd a preliminary injunction against the FLSA updates was granted...inconsistencies across geographical regions When we originally started gathering this information, many assumed...to pursue academic work, and to enhance the sustainability of the scientific enterprise by reducing the...

  19. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ...drug discovery process.” The ClonTracer library originally came about as Bhang and co-workers were trying...treatment process. The authors went on to show that combination treatment with multiple drugs reveals even smaller...heterogeneity to drug discovery and the development of combination therapies.         References 1. Hyo-eun, C. ...

  20. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...AAV serotypes, and the ever-growing variety of recombinase systems. Picking Ligands Picking the right ligand...ligand co-developed by Bryan Roth's and Takafumi Minamimoto's lab called deschloroclozapine (DCZ) shows promise...our chemogenetics poster! This blog post was originally written by Maya Peters Kostman in May 2020 and...
Showing: 401 - 420 of 782 results