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  1. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

    Type
    Blog Post
    Published
    Nov. 14, 2016, 3:30 p.m.
    ...requested from Addgene many times as the technology disseminates throughout the research community. These popular...Takashi Yamamoto. “Addgene has made it possible to eliminate geographical restrictions in life science research...

  2. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    Published
    Nov. 1, 2016, 2:30 p.m.
    ... isolation, insertion and amplification of a recombinant DNA without sequence alteration. Molecular cloning...Annabel HA Parret, and Anastassis Perrakis. "Recombinant cloning strategies for protein expression." Current...

  3. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    Published
    June 29, 2023, 7 p.m.
    ...at one of my first “All Addgene” meetings, when Melina, our CSO and co-founder, approached me and asked...coworkers were only a Slack message away.  When it finally came time for me to present, I thought I would ...

  4. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    Published
    May 4, 2023, 1 p.m.
    ... or have a simple pathology. In these cases, preliminary identification of potential targets by CRISPR...mutations and then test both individual and combinatorial drugs on for effectiveness prior to patient ...

  5. Hot Plasmids - October 2022

    Type
    Blog Post
    Published
    Oct. 6, 2022, 1:15 p.m.
    ... reliable antibodies. It is derived from the C-terminal region of the human proto-oncogene c-Myc. Since... catalog pulled up over 13,000 plasmids! Our recombinant anti-c-Myc [9E10] (isotype IgG2a) antibody is...

  6. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ... ligand from the Bryan Roth lab and Takafumi Minamimoto’s lab known as deschloroclozapine (DCZ) has been...activation Targeting Further restricted to illuminated region Impacts all cells expressing receptors...

  7. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ...Originally published Jan. 14, 2014 and updated Apr. 14, 2020. Any newcomer who joins a molecular biology...types the gene is expressed in and amount of recombinant protein obtained. Selectable Marker The antibiotic...

  8. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...controllable gene knockouts, and inducible gene inactivation or activation in an organoid model (Sun, et ...activity.  As SLs are also known to trigger seed germination of parasitic plants, this approach was also used...

  9. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    Published
    March 31, 2015, 4:08 p.m.
    ...this quality.   I am often asked to give career seminars on Leadership Skills. After attempting to put ...efficiently and with high quality. Their work involves coordination and can require technical mastery. Scientists...

  10. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ... biotech field, let's look at how prokaryotes originally utilized CRISPR/Cas as a powerful and adaptive...expression of 1 of 5 anti-CRISPR genes were found to inactivate the Type I-F CRISPR/Cas system of Pseudomonas...

  12. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    Published
    Feb. 14, 2018, 2:36 p.m.
    ...notes, since the conference, I think I may have finally grasped just how complicated DNA repair pathways...genome editing using oligonucleotide donors". Kan, Yinan, Brian Ruis, Taylor Takasugi, and Eric A. Hendrickson...

  13. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ...beamsplitter, and a 495/30 nm emission filter. This combination results in a 2.6-fold increase in signal relative...fluorescent protein: identification of the molecular determinants of color and applications in fluorescence imaging...

  14. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    Published
    Aug. 31, 2023, 1 p.m.
    ...– I want to understand my obsession with the combination of chocolate and peanut butter), but it’s an ...Using a programmable microcontroller, NeuroStack coordinates optical stimulation of its associated fibers ...

  15. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    Published
    June 9, 2022, 1:15 p.m.
    ...roughly) the same field, often through manuscripts, seminars, or posters. It is important to note that it is...does not explicitly exclude people outside of the original recipients. Instead, it targets a specific audience...

  16. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    Published
    Sept. 21, 2023, 1:15 p.m.
    ...will help get your experiments back on track.  One final note — this post focuses on the species-on-species... Fragment (scFvs) Resources on Addgene.org Recombinant antibodies for IHC at Addgene  ...

  17. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    Published
    June 9, 2016, 2:30 p.m.
    ... THE ORIGINAL NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN RETRACTED AND FOLLOW UP STUDIES HAVE FAILED...prevent the invading genome from “recovering” its original sequence. If you’re looking to disrupt gene function...

  18. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2015, 2:30 p.m.
    ...generic term for any extrachromsomal hereditary determinant”. His proposal, however, was basically ignored... in areas such as bacterial conjugation and recombination, replication and topology, and cloning and gene...

  19. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    Published
    Aug. 25, 2015, 2:30 p.m.
    ...critical three letters after your name (P-H-D).  Finally, having spent your entire career in science, you... you see today is far different than what was originally planned, due in large part to the feedback we...

  20. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    Published
    July 7, 2015, 3:36 p.m.
    ...Bob Goldstein’s lab at the University of North Carolina has made CRISPR in C. elegans even easier - now...elegans genome using Cas9-triggered homologous recombination. Dickinson DJ, Ward JD, Reiner DJ, Goldstein...
Showing: 501 - 520 of 769 results