We narrowed to 802 results for: OMP;

...Gene knockout with CRISPR technology is usually accomplished by Cas9-mediated dsDNA breaks: following a cut... accessibility due to chromatin status). No computational prediction is ever perfect, but this can decrease...to conduct genetic screens, however, a library composed of the latter would likely be more effective, ...

...attached fluorophore. However, since tRNA suppression competes with natural chain termination at stop codons,...contrast can be improved using disulfide-containing compounds such as British anti-Lewisite (which was originally...Vilensky & Redman, 2003]). Most importantly, these compounds cross cell membranes, thus enabling FRET measurements...

...system, developed by the Zhang lab, which has comparable efficiency to the optimized chimeric design but...Where are double strand breaks (DSBs) induced, compared to where the target site sequence (protospacer...how closely homologous the off-target sites are compared to the on-target site, the specific site sequence...

... this segment Hot Plasmid 1: The Tol2 Gateway-Compatible Toolbox for quickly generating zebrafish transgenic.... Listen to Our Segement on the Tol 2 Gateway-Compatible Toolbox  Hot Plasmid 2: AAV Vectors for tageting...

...Get Tips on Verifying Your Plasmid Table 1: Comparing Typical Comericial Ladders with the PSU Ladders...1000 Lanes Cost $200-$500  <$10 $20-30 Cost Comparison: While convenient, commercial ladders cost between...

...experiment. Search engines let you easily find and compare available antibodies from many vendors, while data...Search engine May include validation data  Biocompare Any Any Search engine     Are we missing...

... 1. Lab B needs a redo :-( @SandTBioSci #CompetitiveStreaking @Addgene pic.twitter.com/mk44J4Gcj9 — Dave...species for the challenge, but one species always outcompeted the other species.  When he found the chromoprotein...

...genome usually for a research or applied goal. Components of CRISPR: gRNAs: Sequences of RNA that direct...single base changes in the DNA sequence without completely cutting the DNA. RNA Editors: CRISPR nucleases...

...multinational pharmaceutical company based in Switzerland. After completing her PhD in Chemistry and Chemical...

...decorated office! Below, they turned one of the lab computers into a fireplace scene.  Got our @EmoryChem ...all who participated in the 2018 #DeckTheLab competition. The four winners will receive a holiday care...

...neutralization or targeted destruction.  Antibodies are composed of two light chains and two heavy chains. The ...the use of less toxic adjuvants; 2) generating complex antibody chips that allow assessment of many protein...

...lab equipment on? This applies to water baths, computers, and more. Consolidate your autoclave runs. In...long-term goals to stay motivated and to feel accomplished. In addition to doing some of the quick things...

...measure will ever adequately capture a notion as complex as “scientific impact.” As we look ahead to the...Science - The Book Bollen J, et al. "A Principal Component Analysis of 39 Scientific Impact Measures" (2009...

...’s role in infection also makes it a critical component of vaccines and the target of neutralizing antibodies...Science Translational Medicine have made their complete collection of relevant papers freely available...

...optimal DNA cleavage at 48 °C, a temperature incompatible to genome editing in human cells. The Feng Zhang...efficiency can be controlled by manipulating complementarity between the gRNA and target. Read the paper...

...about careers that we’ve compiled our top career blog posts into one comprehensive, downloadable guide: the...

... more primitive version of the larger and more complex Cas9 and Cas12 proteins. Cas14 cleaves ssDNA But...the detection of RNA, dsDNA, and now ssDNA. By comparing the ability of Cas12 and Cas14 to detect SNPs ...

...finishing your PhD or perhaps you have almost completed a postdoctoral position… or two. You have learned...technique and be able to talk and own these accomplishments. Employers and academic departments want to...

...which decrease the rate of off-target genome edits compared to rat APOBEC1. Now, they’ve engineered SECURE... increased editing efficiency of a target site compared to a mixture of two base editor enzymes in mammalian...

...gRNAs to knockout genes – are essential screening components, the model system and the assay are just as critical...him that he has the best words and fingers of a completely normal size.      References: 1. Doench, John...