962 results

Plasmid addiction systems, used by bacteria to maintain plasmids in dividing cells, have been developed by scientists as molecular biology tools.

Nov. 1, 2018, 12:35 p.m.

Learn about the different types of stem cells in this guide. In particular, we discuss the pros and cons of using embryonic stem cells and adult stem cells and using induced pluripotent stem cells (iPSCs) as a model for regenerative medicine.

Oct. 30, 2018, 1:04 p.m.

Find tips for transforming arabidopsis with your plasmid construct of interest.

Oct. 25, 2018, 1:23 p.m.

Get tips for producing, purifying, and tittering AAV, a tick but useful viral vector of choice for many people working to express genes in mammalian cells.

Oct. 23, 2018, 11:49 a.m.

In Vivo Assembly (IVA) cloning uses the bacterial recombination pathway to allow any cloning procedure to be performed with a simple two-step, 2 hr protocol prior to transformation. This post covers the basics of the IVA cloning procedure, primer design, and tips & tricks for successful IVA cloning.

Oct. 18, 2018, 12:37 p.m.

Learn a variety of techniques you can use increase the fidelity of multi-part Golden Gate Assembly reactions.

Oct. 11, 2018, 12:30 p.m.

Erik Snapp, Director of Graduate and Postdoctoral Programs at Janelia Research Campus, gives advice on how to complete a successful postdoc and set yourself on a path to a successful academic career.

Oct. 9, 2018, 12:21 p.m.

The labs of John Doench and David Root have recently created CRISPR libraries to knock out, inactivate, or activate genes for genome-wide screens. These optimized libraries are effective for probing gene function while using fewer sgRNAs.

Oct. 4, 2018, 12:44 p.m.

Get tips on choosing the best lab for your graduate or postdoc work.

Oct. 2, 2018, 12:56 p.m.

Learn why codon usage bias exists and techniques you can use to avoid the detrimental affects of codon usage bias on heterologous protein expression - codon optimization and expression of alternative tRNAs.

Sept. 27, 2018, 1:09 p.m.

Addgene has recently reached the 1 million plasmids shared milestone. This post shares some of the social media around this feat and answers the questions: how much space does 1 million vials containing plasmids take?

Sept. 25, 2018, 2:39 p.m.

Learn how the Personal Genetics Education Project (pgEd) engages with various communities on biological science and technology. Get tips on how to work at a similar organization from pgEd's director of new initiatives, Johnny Kung.

Sept. 20, 2018, 12:17 p.m.

Learn all about how to regulate cell growth dynamics with optogenetics, the Riboglow platform for visualizing RNA production and localization, mCarmine, a new and improved red fluorescent protein, and the ACE reporter system for quantifying base-editing and Cas9 cleavage activity.

Sept. 18, 2018, 1:33 p.m.

Genome-wide CRISPR/Cas9 screens are a high-throughput systematic approach for identifying genes involved in a biological process. Recently the Bassik Lab at Stanford developed a new genome-wide CRISPR knockout screen to analyze unanswered questions about CRISPR screen design by including safe targeting guides and short gRNAs.

Sept. 13, 2018, 1:55 p.m.

Learn the basics of CRISPR ribonucleoprotein (RNP) preparation and delivery for your next gene editing experiment.

Sept. 6, 2018, 12:02 p.m.

Learn how the Journal of Emerging Investigators enables high school students to learn more about the scientific process and scientific publishing.

Sept. 5, 2018, 12:01 p.m.

Gene expression is often imprecise due to variations in gene copy number within the cell (either on plasmid or genome). New promoters from Chris Voigt’s lab enable stable gene expression regardless of copy number regardless of growth phase and whether they are expressed on the plasmid or chromosome.

Sept. 4, 2018, 12:53 p.m.

Though microbes are microscopic, there are many ways to visualize microbes. In this blog post, we summarize the latest tools in science communication to showcase microbes - foldscopes, agar art, and GIFs have all been recently use to bring microbiology to diverse audiences.

Aug. 28, 2018, 12:04 p.m.

GFP is the most widely used fluorescent protein, but there are several instances that limits its use. GFP requires oxygen, is sensitive to acid, and its large size can be problematic. When thinking about ways to label proteins, a different probe may be more appropriate.

Aug. 23, 2018, 12:05 p.m.

There are many plasmid tools available in molecular biology so how do you chose the right cloning method for you? Here, consider speed, cost, difficulty, efficiency, and the number of inserts when designing your cloning strategy.

Aug. 21, 2018, 12:31 p.m.