966 results

Gene expression is often imprecise due to variations in gene copy number within the cell (either on plasmid or genome). New promoters from Chris Voigt’s lab enable stable gene expression regardless of copy number regardless of growth phase and whether they are expressed on the plasmid or chromosome.

Sept. 4, 2018, 12:53 p.m.

Though microbes are microscopic, there are many ways to visualize microbes. In this blog post, we summarize the latest tools in science communication to showcase microbes - foldscopes, agar art, and GIFs have all been recently use to bring microbiology to diverse audiences.

Aug. 28, 2018, 12:04 p.m.

GFP is the most widely used fluorescent protein, but there are several instances that limits its use. GFP requires oxygen, is sensitive to acid, and its large size can be problematic. When thinking about ways to label proteins, a different probe may be more appropriate.

Aug. 23, 2018, 12:05 p.m.

There are many plasmid tools available in molecular biology so how do you chose the right cloning method for you? Here, consider speed, cost, difficulty, efficiency, and the number of inserts when designing your cloning strategy.

Aug. 21, 2018, 12:31 p.m.

Learn how you can use RaPID to detect RNA-protein interactions and its applications in infectious and human genetic diseases.

Aug. 16, 2018, 12:40 p.m.

Learn how you can use the Spaghetti Monster fluorescent protein (smFP) tags for neuronal tracing and multicolor labeling of brain structures.

Aug. 14, 2018, 12:42 p.m.

Learn how AAVs can be used to trace neuronal connections.

Aug. 9, 2018, 1:04 p.m.

Find tips and best practices for producing lentivirus in your lab: from choosing a packaging system to optimizing transfection and improving titers.

Aug. 7, 2018, 12:31 p.m.

Learn how you can use the Biobits plasmids and freeze-dried cell extracts to teach students about molecular biology.

Aug. 2, 2018, 12:56 p.m.

Learn how and why Adeno-associated virus (AAV) has enabled breakthroughs in retinal gene therapy.

July 31, 2018, 12:55 p.m.

Learn how you can use kinase translocation reporters to monitor kinase activity at the single cell level.

July 26, 2018, 12:46 p.m.

Gamma-retroviral vectors and lentiviral vectors can be used in a wide variety of experiments including those involving genome engineering, RNAi, and inducible gene expression. In this post, learn about some of the most popular viral vectors available from Addgene and their uses.

July 24, 2018, 12:51 p.m.

Find great science communication tips from Nathan Sanders of ComSciCon, a communicating science conference series for graduate students.

July 19, 2018, 1:12 p.m.

Learn how you can use the Build Your Plasmid card game to teach undergraduate students about genetic engineering.

July 17, 2018, 12:23 p.m.

Learn some best practices to keep your mammalian cell lines free of contaminants and your experiments running smoothly.

July 12, 2018, 1:09 p.m.

Learn how electronic lab notebooks can make your lab life a little easier and find tips to choose the best the electronic lab notebook for your needs.

July 10, 2018, 1:08 p.m.

Learn about the csRNP epitope tagging method - a new and efficient way to tag endogenous genes using CRISPR.

June 28, 2018, 3:01 p.m.

Learn all about a highly processive reverse transcriptase, a new nanobody toolkit, and CRISPR-based tools for monitoring protein-DNA interactions

June 27, 2018, 1:16 p.m.

Congratulations to Paula Videira, Michael McMurray, & Richard Gronostajski who each are being awarded free plasmids form Addgene. Read on to learn how these researchers plan to use plasmids for research on diseases of glycosylation, arginine mutagenesis, & neural development.

June 26, 2018, 1:33 p.m.

Learn how to use the dTAG system to inducibly degrade your protein of interest.

June 21, 2018, 2:06 p.m.