965 results

This post describes the design of shuttle vectors, which are designed for replication, selection, and expression in more than one host species.

May 20, 2025, 1:15 p.m.

This post describes the biology and use of integrase-deficient lentiviral vectors in comparison to standard lentiviral vectors.

May 13, 2025, 1:15 p.m.

Learn about Addgene's QC process and how and why we maintain a high standard of sequence accuracy across our plasmid collection.

May 6, 2025, 1:15 p.m.

Addgene adds new ready-to-use viral vectors to our service every month. Read all about the latest additions here!

April 29, 2025, 1:30 p.m.

Addgene remains steadfast in our mission to accelerate research and discovery by improving access to high-quality scientific materials and information. Our agility as a nonprofit resource can prove to be vital during this period of constrained funding.

April 23, 2025, 1:30 p.m.

This post covers loading controls and normalization of semi-quantitative western blots. We cover housekeeping proteins and total protein loading controls.

April 8, 2025, 1:15 p.m.

This post describes CRISPR-derived base editors that catalyze base transversions, such as purines like A or G to pyrimidines like T or C.

April 1, 2025, 1:15 p.m.

This post describes CRISPR-derived base editors to convert cytosine to adenine or guanine, called CGBEs or GBEs.

March 25, 2025, 1:15 p.m.

Learn about how CRISPR systems are classified. Addgene's guide to typing CRISPR.

March 18, 2025, 1:15 p.m.

Check out the latest Hot Plasmids, viral vectors, and antibodies in the Addgene repository!

March 11, 2025, 1:15 p.m.

Addgene Executive Director Chonnettia Jones reflects on highlights of 2024 and shares exciting plans for 2025.

Feb. 25, 2025, 2:15 p.m.

An overview of cytosine and adenine base transition editors, which make precise changes in DNA bases without causing double-strand breaks.

Feb. 13, 2025, 2:15 p.m.

Learn about how Cascade-Cas3 can be used as a CRISPR tool to degrade large fragments of DNA.

Feb. 11, 2025, 2:15 p.m.

Changes in export control regulations have make rabies and VSV-G plasmids more accessible to researchers around the world.

Feb. 6, 2025, 2:15 p.m.

Learn about the advantages of using AAV as a delivery method for CRISPR screens.

Feb. 4, 2025, 2:15 p.m.

Addgene adds new ready-to-use viral vectors to our service every month. Read all about the latest additions here!

Jan. 28, 2025, noon

This post provides tips for designing a prime editing experiment, from pegRNA design, PE tool selection, and choice of DNA sequence to edit.

Jan. 23, 2025, 2:15 p.m.

Learn about the most common injection routes in mice, including their key differences and uses.

Jan. 22, 2025, 9:42 p.m.

Making the transition from bench to management can be daunting, but these tips can help!

Jan. 21, 2025, 2:15 p.m.

Adeno-associated virus (AAV) vector serotypes can help you target specific cell types through their tissue tropism.

Jan. 16, 2025, 2:15 p.m.