We narrowed to 50 results for: codon optimized

1. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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   Blog Post

   Published

   Feb. 4, 2020, 2:15 p.m.

   ...manipulations start. Anthony Forster’s lab used codon optimization programs to engineer 14 chromoprotein sequences...

2. CRISPR 101: RNA Editing with Cas13

   Type

   Blog Post

   Published

   July 31, 2020, 12:30 p.m.

   ...pathogenic alleles, or introduce a premature stop codon to render an RNA nonfunctional. In their quest to...directed evolution across the full ADAR2DD to further optimize cytidine deaminase activity. While RESCUE can ...

3. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

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   Blog Post

   Published

   July 12, 2021, 1:15 p.m.

   ...SARS-CoV-2 ORFs and include plasmids that are codon optimized. Browse the collection or learn more about ...

4. Tips for Screening with Yeast Two Hybrid Systems

   Type

   Blog Post

   Published

   Oct. 22, 2015, 2:30 p.m.

   ...20805792. 7. Stynen B., Van Dijck P., Tournu H. A CUG codon adapted two-hybrid system for the pathogenic fungus...channel interactions detected by a genetic system optimized for systematic studies of membrane protein interactions...

5. Optogenetics + CRISPR, Using Light to Control Genome Editing

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   Blog Post

   Published

   Sept. 3, 2020, 12:15 p.m.

   ...develop an optimized photoactivatable CRISPR gene activation system, but settled on a different optimal fusion...researchers tested a variety of combinations to optimize both fusion proteins. The best performing combination...fusion protein combination. The optimized LACE system consisted of: 1) CIBN-dCas9-CIBN, where CIBN is the... would add the PCK upon reaching the amber stop codon, TAG. (To learn more about site-specific incorporation...

6. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

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   Blog Post

   Published

   Oct. 11, 2016, 2:30 p.m.

   ...enhance targeting to the nucleus, and several codon optimized Cas9 variants have been created in an effort...

7. RNA Interference in Plant Biology: New Tools for an Old Favorite

   Type

   Blog Post

   Published

   Oct. 27, 2020, 1:15 p.m.

   ... to the use of exon skipping, alternative start codons, and/or the phenomenon of transcriptional adaptation...and robust gene silencing (following initial optimization of the amiRNA). Although downstream work is ...

8. Plasmids 101: Using Transposons in the Lab

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   Blog Post

   Published

   July 6, 2021, 1:28 p.m.

   ... active in mammalian cells compared to the codon-optimized wild-type piggyBac transposase.  piggyBac ...

9. Hot Biosensors 2022: Year-End Roundup

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   Blog Post

   Published

   Dec. 6, 2022, 2:15 p.m.

   ...synaptic glutamate response. They generated variants optimized for different applications (v82 has higher affinity...indicator (GEVI) called JEDI-2P (Liu et al. 2022) optimized for two-photon imaging. They use a unique and ...multiparameter screen for accelerated development and optimization of soluble genetically encoded fluorescent biosensors...variety of imaging conditions (155 μs and <0.1 °C in optimal conditions). While B-gTEMP itself is a significant...viral prep here: pAAV.hSyn.FLEX.iGluSnFR3.v857.PDGFR.codonopt    Aggarwal A, et al. Glutamate indicators ...

10. CRISPR 101: Cytosine and Adenine Base Editors

    Type

    Blog Post

    Published

    Feb. 13, 2025, 2:15 p.m.

    ...lab modified the nuclear localization signals and codon usage BE4 to create BE4max and AncBE4max with a ...cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nature Biotechnology...