We narrowed to 706 results for: plasmid

An introduction to the use of antibodies in immunoprecipitation, including antibody immobilization, controls, and a basic overview of techniques.

Dec. 7, 2021, 2:15 p.m.

Choosing a CRISPR activator? This article features four of the most popular CRISPR activators (dCas9-VP64, SAM, SunTag, and VPR) and some newer activators like dCas9-CBP and SPH.

Oct. 6, 2020, 1:15 p.m.

Marc Zimmer's book, The State of Science, examines broad issues in science including racism and sexism, peer-review, funding, CRISPR, and the politicization of science.

July 30, 2020, 1:15 p.m.

Learn all about CAPTURE - a new CRISPR-based technique to study regulatory elements in their native chromatin contexts.

July 2, 2020, 12:40 p.m.

Find tips and tricks for using digital droplet PCR to quantify your AAV titers.

Jan. 16, 2020, 2:18 p.m.

Crossing the blood brain barrier is difficult for many AAVs. But, PHP.B has impressive CNS transduction in some mice. Learn more about how PHP.B and about its strain specific performance.

July 23, 2019, 12:46 p.m.

Electronic lab notebooks (ELNs) are useful tools that help researchers keep track of data. However, not all ELNs support the same functionality when it comes to reproducibility.

Jan. 17, 2019, 1:38 p.m.

The labs of John Doench and David Root have recently created CRISPR libraries to knock out, inactivate, or activate genes for genome-wide screens. These optimized libraries are effective for probing gene function while using fewer sgRNAs.

Oct. 4, 2018, 12:44 p.m.

Learn how and why Adeno-associated virus (AAV) has enabled breakthroughs in retinal gene therapy.

July 31, 2018, 12:55 p.m.

Learn how you can use Synthego's ICE tool to analyze your CRISPR genome editing experiments.

May 8, 2018, 1 p.m.

Learn all about mesothelioma and upcoming immunotherapy treatments for this devastating form of cancer.

Dec. 13, 2016, 3:30 p.m.

Luke Lavis from Janelia Farms describes how small molecule fluorophores can still be useful in the age of fluorescent proteins.

Sept. 8, 2016, 2:30 p.m.

Learn how to design a CRISPR screening experiment targeting enhancers.

Aug. 9, 2016, 2:30 p.m.

Learn about the founding the science communication nonprofit (CINSO) and get tips on thinking about your science career.

Feb. 25, 2016, 3:30 p.m.

Learn how the team building atmosphere at Addgene keeps its employees excited about helping researchers share their science.

Sept. 3, 2015, 2:30 p.m.

If flow plots seem confusing, our guide to understanding them is here to help!

Feb. 6, 2024, 3:13 p.m.

Learn how to verify indels, deletions, and homology directed repair (HDR) events in your CRISPR modified cell lines.

Nov. 3, 2022, 12:15 p.m.

Genome-wide CRISPR/Cas9 screens are a high-throughput systematic approach for identifying genes involved in a biological process. Recently the Bassik Lab at Stanford developed a new genome-wide CRISPR knockout screen to analyze unanswered questions about CRISPR screen design by including safe targeting guides and short gRNAs.

Sept. 13, 2018, 1:55 p.m.

Learn how electronic lab notebooks can make your lab life a little easier and find tips to choose the best the electronic lab notebook for your needs.

July 10, 2018, 1:08 p.m.

Learn how we titer AAV for our viral service at Addgene and find tips for improving your AAV tittering methods.

Nov. 15, 2017, 3 p.m.