We narrowed to 706 results for: plasmid

Learn how to optimize CRISPR-activated homology directed repair to make targeted edits in mammalian cells.

Sept. 5, 2017, 1:58 p.m.

Find 10 tips on how to give the perfect science talk

Aug. 23, 2016, 2:30 p.m.

Discover the best situations to use Cas9 activators in your experiments with tips from their creators.

Aug. 18, 2016, 2:30 p.m.

Cell culture can be difficult work, follow these tips to keep your cell lines free of contamination.

May 5, 2016, 2:30 p.m.

Lentiviral transductions can be frustrating. Follow these tips to achieve consistently successful lentiviral transductions.

April 7, 2016, 2:30 p.m.

This post, written by the creator of the CRISPRecise kit, explains how to use it to minimize off-target effects in CRISPR genome editing.

March 31, 2026, 1:15 p.m.

Baby KJ was treated for his devastating genetic disorder with CRISPR base editing. This post describes the prior advances that made his cure possible.

Sept. 16, 2025, 1:15 p.m.

This post describes CRISPR-derived base editors that catalyze base transversions, such as purines like A or G to pyrimidines like T or C.

April 1, 2025, 1:15 p.m.

Introduction to immunofluorescence and how you can use it to illuminate your research.

Nov. 16, 2021, 2:15 p.m.

Looking for a way to study integrins? IPI's first deposit, a toolset comprised of anti-integrin antibodies, is now available in Addgene's repository!

March 28, 2024, 1:15 p.m.

Overview of split vector approaches for fitting genes >4.7 kB into AAV including overlapping, transplicing, hybrid, and sequential homologous recombination.

Sept. 1, 2020, 1:15 p.m.

GFP is the most widely used fluorescent protein, but there are several instances that limits its use. GFP requires oxygen, is sensitive to acid, and its large size can be problematic. When thinking about ways to label proteins, a different probe may be more appropriate.

Aug. 23, 2018, 12:05 p.m.

We describe the different classes of optogenetic tools available and what levels of control they give you in your experimental setup.

June 27, 2023, 1:15 p.m.

Practical advice for using CRISPRs: designing your gRNA and introducing it into cells.

Sept. 24, 2020, 1:15 p.m.

Learn how you can use Green Listed to design gRNAs for custom CRISPR screens.

July 11, 2017, 2:30 p.m.

Learn about some of the factors you should take into consideration when choosing the appropriate AAV for your next experiment.

June 13, 2017, 2:30 p.m.

Learn about the pros and cons of biased and unbiased CRISPR genotyping methods.

Oct. 4, 2016, 2:30 p.m.

Get an overview of flow cytometry including how it works, what you can use flow cytometry for, and tips and tricks for a successful flow cytometry run.

July 20, 2021, 1:15 p.m.

Get tips on choosing the best fluorescence microscopy technique for your next imaging experiment.

Oct. 10, 2017, 1:57 p.m.

Cancer immunotherapies hold great promise for treating many different types of cancer. Learn more about this promising new way to fight cancer.

March 14, 2017, 2:30 p.m.