We narrowed to 251 results for: cas9

Learn about CRISPR plasmids at Addgene such as an E. coli genome-wide CRISPRi library, GeneWeld vectors, CasX plasmids, and drug inducible CRISPR/Cas9 activation.

Sept. 10, 2019, 12:56 p.m.

This post discusses the applications, advantages, and caveats to using hybrid guide RNAs, in which DNA bases are substituted for RNA bases.

Jan. 27, 2026, 2:14 p.m.

iTOP is a protein-only transduction method for delivering proteins and RNA into a variety of cell types, with potential applications with CRISPR/Cas9.

June 23, 2015, 8:37 p.m.

Baby KJ was treated for his devastating genetic disorder with CRISPR base editing. This post describes the prior advances that made his cure possible.

Sept. 16, 2025, 1:15 p.m.

Genome-wide CRISPR/Cas9 screens are a high-throughput systematic approach for identifying genes involved in a biological process. Recently the Bassik Lab at Stanford developed a new genome-wide CRISPR knockout screen to analyze unanswered questions about CRISPR screen design by including safe targeting guides and short gRNAs.

Sept. 13, 2018, 1:55 p.m.

The labs of John Doench and David Root have recently created CRISPR libraries to knock out, inactivate, or activate genes for genome-wide screens. These optimized libraries are effective for probing gene function while using fewer sgRNAs.

Oct. 4, 2018, 12:44 p.m.

Cas13a is an RNA cleaving enzyme that can be used to detect specific RNAs. Learn about this and other applications, like nucleic acid detection.

Sept. 21, 2017, 2:07 p.m.

Overview of polycistronic glycine tRNA-gRNA (PTG) multiplexing gRNA cassettes, with comparisons to Pol III and Cys4 gRNA cassettes.

June 2, 2015, 6:06 p.m.

X-CHIME, a series of CRISPR tools developed and deposited by Arlene Sharpe's lab, allows for germline knockouts in immune cells in mice.

Jan. 16, 2024, 6:13 p.m.

Learn about the latest CRISPR plasmids deposited at Addgene such as an E. coli genome-wide CRISPR inhibition library, SECURE base editors, and Nme2Cas9.

May 28, 2019, 12:49 p.m.

Learn how to optimize CRISPR-activated homology directed repair to make targeted edits in mammalian cells.

Sept. 5, 2017, 1:58 p.m.

Designing a CRISPR experiment in bacteira? Check out these tools for genome engineering, CRISPRa, CRISPRi, base editing, and microbiome engineering.

Sept. 28, 2020, noon

New CRISPR pooled libraries, such as SAM, CRISPRa, and CRISPRi, offer new ways to do genome-wide functional screens.

Feb. 24, 2015, 7:50 p.m.

This post describes CRISPR-derived base editors to convert cytosine to adenine or guanine, called CGBEs or GBEs.

March 25, 2025, 1:15 p.m.

Learn about TnpB, a small genome editor with a function similar to CRISPR systems!

July 29, 2025, 1:15 p.m.

Check out the latest Hot Plasmids, viral vectors, and antibodies in the Addgene repository!

Nov. 21, 2024, 1:30 p.m.

Learn how to use in vitro screening and other tricks to expedite the CRISPR genome editing process in mice.

June 28, 2016, 10:59 a.m.

Hear from the authors about the generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9.

Feb. 18, 2015, 3:09 p.m.

Learn how you can use HDR and CRISPR to make a knock-in cell line for your research.

Dec. 22, 2022, 2:15 p.m.

The CRISPR babies saga shows us that we need action to move forward in bringing CRISPR technologies from bench to bedside.

Dec. 20, 2018, 1:15 p.m.