We narrowed to 161 results for: grna

Check out all of the plasmids featured in our hot plasmids articles from 2016.

Dec. 22, 2016, 3:03 p.m.

A review of the CasPEDIA resource and its features.

Jan. 25, 2024, 2:15 p.m.

Learn how to use Easi-CRISPR to generate mouse knock-in and conditional allele models.

April 5, 2018, 12:42 p.m.

Addgene reached its 15 year anniversary on January 8, 2019. In this blog post, find the top 15 requested plasmids since our founding. Many of these include lentiviral or CRISPR tools!

Jan. 8, 2019, 1:54 p.m.

Various lentiviral CRISPR-Cas9 libraries have been developed to perform genome-wide knockout screens, including the GeCKO library from Feng Zhang's lab.

Feb. 20, 2014, 4:57 p.m.

Enjoy 12 new CRISPR articles from Addgene's 12 Days of CRISPR!

Dec. 22, 2020, 2:15 p.m.

Learn about xCas9, an engineering Cas9 variant with broad PAM flexibility and high on-target specificity.

March 28, 2018, 6:52 p.m.

Dr. Le Cong, first author on a seminal CRISPR article from Dr. Feng Zhang's lab, answers FAQs about the genome editing technology.

March 13, 2014, 4:08 p.m.

Learn about how Cascade-Cas3 can be used as a CRISPR tool to degrade large fragments of DNA.

Feb. 11, 2025, 2:15 p.m.

PRIDICT 2.0 is an updated software that can help you design gRNAs for PRIME editing. Read more in our guest post!

June 25, 2024, 1:15 p.m.

With many different Cas9 variants out there, it can be hard to know which one to use in your particular CRISPR experiment. This post is here to help.

Oct. 13, 2022, 1:45 p.m.

Discover the best situations to use Cas9 activators in your experiments with tips from their creators.

Aug. 18, 2016, 2:30 p.m.

Here, we cover four different plasmid screening strategies including blue-white screening, colony PCR, restriction digest analysis, and Sanger sequencing.

Sept. 15, 2020, 1:15 p.m.

Learn about some newly developed Cas proteins and how to choose a Cas protein based on site accessibility, specificity, and sensitivity.

Nov. 5, 2019, 1:28 p.m.

Cas14 was discovered by the Doudna lab. It cleaves ssDNA (but not dsDNA) in trans and can be adapted for a diagnostic toolkit such as DETECTR.

Nov. 29, 2018, 1:53 p.m.

Find the latest plasmids and viral vectors to available at Addgene in the October 2020 hot plasmids blog post!

Oct. 13, 2020, 1:15 p.m.

Overview of split vector approaches for fitting genes >4.7 kB into AAV including overlapping, transplicing, hybrid, and sequential homologous recombination.

Sept. 1, 2020, 1:15 p.m.

Three studies recently showed that CRISPR can be used to treat Duchenne muscular dystrophy in mice. Learn about the work and obstacles to application.

Jan. 26, 2016, 3:30 p.m.

Did you know CRISPR can be used to target non-coding RNAs? In this blog post, we explain the pros and cons of different approaches.

Nov. 14, 2024, 2:15 p.m.

Mobile-CRISPRi is a set of modular CRISPRi tools that can be used in diverse bacteria from various environments. It has the advantage of stable genomic integration and is easily transferred via conjugation.

April 4, 2019, 12:53 p.m.