965 results

Tips to help you save time when using the the Basic Local Alignment Search Tool (BLAST) provided by NCBI to compare sequence results.

May 29, 2014, 1:29 p.m.

Tips to consider when publishing a scientific manuscript, including what type of journal to publish in, peer review, and helpful dos and don'ts.

May 22, 2014, 4:02 p.m.

Tips to help you choose which fluorescent protein to use in your experiments.

May 20, 2014, 2:06 p.m.

Green fluorescent protein (GFP) and its derivatives can be used experimentally as fusion tags, biosensors, reporters, and more.

May 15, 2014, 3:33 p.m.

Ten ways to begin sharing your science with your community, from talking to writing to tweeting.

May 13, 2014, 8:34 p.m.

Interested in how other labs celebrate accomplishments and have fun outside the lab? We've interviewed 3 PIs to learn about how their labs celebrate.

May 8, 2014, 3:07 p.m.

New fluorescence resonance energy transfer (FRET)-based calcium biosensors and FRET-based sensors for the intracellular imaging of transition metal ions.

May 6, 2014, 1:07 p.m.

Addgene explains what a material transfer agreement is, how it can benefit you and your academic institution, and how Addgene makes MTAs easy.

May 1, 2014, 8:23 p.m.

Advice on starting a lab from three PIs who have successfully built their own academic labs.

April 29, 2014, 1:43 p.m.

Addgene answers questions about lentiviral plasmids, including 2nd vs 3rd generation transfer vectors, packaging plasmids, biosafety concerns, and more.

April 23, 2014, 1:08 p.m.

Tips for how to choose a research project in a life science lab.

April 17, 2014, 2:45 p.m.

Tips for being more environmentally friendly in a life science research lab.

April 15, 2014, 3:11 p.m.

Advice for scientists choosing a lab, including interviews with Principal Investigators and important factors to consider in your decision.

April 8, 2014, 2:50 p.m.

The next post in the Plasmids 101 series focuses on the promoter region, the element responsible for initiating the transcription of your insert into RNA.

April 3, 2014, 8:05 p.m.

The first in a series of blog posts in which PIs give advice about achieving success in academia, choosing you research topic, and more.

April 1, 2014, 5:33 p.m.

In vivo imaging is used to study individual plasmids or protein-protein interactions in organs and whole mammals.

March 27, 2014, 5:14 p.m.

Read about how mammalian plasmids differ from their bacterial counterparts, including how replication occurs and whether selection is necessary for transfected cells.

March 25, 2014, 3:15 p.m.

Ten tips to help scientists negotiate their work/life balance from Joanne Kamens, Addgene's Executive Director.

March 20, 2014, 3:04 p.m.

Dr. Le Cong, first author on a seminal CRISPR article from Dr. Feng Zhang's lab, answers FAQs about the genome editing technology.

March 13, 2014, 4:08 p.m.

CRISPR-Cas9 technology appears in numerous publications, including its use for genome editing in monkeys and x-ray crystallography of Cas9's 3-D shape.

March 11, 2014, 5:55 p.m.