Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 202 results for: aav

Showing: 81 - 100 of 202 results

  1. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...specifically AAV, are also being exploited as delivery mechanisms for gene therapy. The tractability of AAV, low...of virus to choose from (lentivirus, retrovirus, AAV, etc.). When you’ve decided on an application, our...00487-6 Additional Resources on the Addgene Blog AAV: A Versatile Viral tool for Gene Expression in Mammals...Listen to Connie Cepko Discuss Gene Therapy with AAV Adenoviral Delivery of CRISPR/Cas9 Aims to Expand...

  2. Clear and Accessible Writing

    Type
    Blog Post
    Published
    Feb. 15, 2024, 2:15 p.m.
    ...sighted readers.    Figure 1: An image from AAV Purification by Iodixanol Gradient Ultracentrifugation...image in Figure 1 appears on our protocol page for AAV Purification by Iodixanol Gradient Ultracentrifugation...vectors for the first time and learning how to purify AAV.  Second, you’ll identify your purpose in using that...the technical details of collecting the purified AAV–that the interfaces between layers are blurred after...are captured at the 40% layer. To collect purified AAV, insert a syringe at the interface of the 40% layer...

  3. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ... it is too large to be effectively packaged into AAV particles for delivery into mammalian cells, especially...archaea are much smaller and would be ideal for such AAV packaging, but their activity in mammalian systems... Cas12a based systems, making it well suited for AAV packaging. Most importantly, this CasMINI system ...effective at genome editing when delivered in an AAV system, and demonstrated that the new system was ...methods, including plasmid, ribonucleoprotein, and AAV.  Read the paper in Nature Chemical Biology Find...

  4. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...Cpf1 multiplexing: Transfection, lentivirus, and AAV Cpf1’s ability to process its own pre-crRNA arrays...platforms: transfection, lentiviral transduction, and AAV transduction.  In most of Zetsche et al’s experiments...array that can be processed by Cpf1. Lastly, an AAV vector was used to multiplex edit 3-4 genes in a ...experiments, cells were infected with a 1:1 ratio of two AAVs,  one expressing Cpf1 and the other a crRNA array...Experiment A Match Made in Heaven: CRISPR/Cas9 and AAV Resources on Addgene.org Browse Our CRISPR Multiplexing...

  5. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ...and adeno-associated virus (AAV). The least risky of these four viruses is AAV – it is not known to cause...integrate at chromosome 19q13.4 qtr. (AAVS1)). For these reasons, AAV is usually classified as a BSL-1 safety...safety level. Adenovirus is similar to AAV in that it does not integrate into the host’s genome. However...bleach (plastic) or ethanol (other surfaces). For AAVs, which are resistant to ethanol and isopropyl alcohol...

  6. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    Published
    Nov. 3, 2020, 2:15 p.m.
    ...optogenetic experiments. Intracranial injections of AAV are commonly used to deliver channelrhodopsins to...collected following intracranial injection of an AAV expressing a ChRger. In both experiments, neurons...Designer channelrhodopsins paired with a systemic AAV allow for minimally-invasive optogenetics While ChRgers...delivered ChRgers by intravenous injection of PHP.eB, an AAV capsid capable of broad transduction of the nervous... on the Addgene blog Read more about the PHP.eB AAV capsid Read more about optogenetics Learn about the...

  7. Download Science-Themed Zoom Backgrounds from Addgene

    Type
    Blog Post
    Published
    April 22, 2021, 1:15 p.m.
    ...service provides ready-to-use AAV and lentiviral preps! Download the green AAV background.       Some of...the image background.     If you’re working with AAV or lentivirus in your research, you might want to... and needed a break so they're hanging out with Aavery in this Zoom video background. Want the purple ...bacteria video background with mascots Blugene and Aavery? Download the 720p version or download the 1080p...

  8. The Addgene Antibody Data Hub

    Type
    Blog Post
    Published
    Sept. 12, 2024, 1:15 p.m.
    ...Collection Addgene's AAV Data Hub More resources on the Addgene blog Newly Updated AAV Data Hub Antibodies..."Pass". Curating the data reports Just like our AAV Data Hub, each data submission we receive is checked...

  9. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    Published
    Feb. 23, 2023, 2:15 p.m.
    ...also rolled out an updated AAV Data Hub to share curated information about AAV usage in research applications...applications. Scientists around the world can upload AAV validation data, sharing what works and, equally importantly...

  10. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ... AAV8, as well as AAV5 and AAVrg. Find the Cre-expressing viral preps here AAV-CAG-dLight1.3b from ...in serotype AAV5! Get dLight1.3b here Looking for a knock-out in your experiments? AAV-flex-taCasp3...available as a ready to use viral prep in serotype AAV5. Find AAV-flex-taCasp3-TEVp here The red-shifted channelrhodopsin...SaCas9 ABE variants. It's also one of the smallest AAV-deliverable ABE.  Find the plasmid here Jesse Zalatan...the Deisseroth viral preps here Coming soon! New AAV serotypes from the Sanford Boye lab with enhanced...include AAV1, and AAV9. Find viral preps here pAAV-hSyn-Cre-P2A-dTomato is now available in AAV1 and AAV8...Loren Looger Lab now available in AAV1. Get GFAP.SF-iGluSnFR.A184S-AAV1 here New Dual and Triple feature...

  11. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    Published
    Sept. 24, 2015, 2:30 p.m.
    ...elements are inserted into an AAV transfer vector and used to generate AAV particles. Transient or stable...for conducting genome-wide screens using CRISPR. AAV transduction Only compatible with SaCas9 (packaging...or gRNA. Infects dividing and non-dividing cells. AAV is least toxic method for in vivo viral delivery....hard Viral delivery   Lentivirus Retrovirus AAV Cells Dividing or nondividing Dividing Dividing...

  12. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ...future experiments.  Use Addgene’s AAV Data Hub. You can search the AAV Data Hub for tools to use in future...experiments. If you’ve used Addgene’s AAV tools, you can contribute data to the AAV Data Hub to help the research...

  13. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...Addgene.org AAV Vector for TVA Expression AAV for Rabies Glycoprotein (G) expression  Find Retrograde AAV ...viruses typically used in neuroscience research (e.g., AAV) are useful for labeling the individual neurons they...17179932. Additional Resources on the Addgene Blog AAVs CREATed for Gene Delivery to the CNS and PNS Advanced...

  14. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    Published
    Aug. 10, 2021, 1:15 p.m.
    ...currently uses ddPCR for AAV titrating. You can learn all about ddPCR for AAV titration and find helpful...tricks in our blog post “Droplet Digital PCR for AAV Quantification.”   Multiplex PCR Multiplex PCR, as... nucleotide concentration of starting material AAV Titrations Allelic Variations Quantifying low abundance...resources on the Addgene blog Droplet Digital PCR for AAV Quantification Plasmid cloning by PCR  Plasmids 101...

  15. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ...distributing a small but growing catalog of ready-made AAV and Lentiviral preps. This new Viral Service represents... lab from the Allen Institute have supported the AAV initiative from the beginning and have worked closely...Research Campus have provided valuable insight into AAV production. Finally, we are blessed to have an incredibly...Blog Check Out the Top Requested Lentivirus and AAV of 2016 Learn How You Can Use the Viral Service in...

  16. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...with AAV-S5E2-GCaMP6f, now available as a ready-to-use viral prep in AAV1!  New ready-to-use AAV prep ...Plasmids for delivering CRISPRi components by a single AAV vector are now available from Tonia Rex's lab.  vfCRISPR...Gether lab, now available as ready-to-use AAV vectors.   ...Rylan Larsen lab, now available in a ready to use AAV5 viral prep.   Flp-dependent DREADDs from the Gether...

  17. Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Feb. 25, 2025, 2:15 p.m.
    ...response to a demand for more AAV viral vector options, we launched AAV Packaged on Request, a service...simplifies your viral vector options. Almost any AAV transfer plasmid in our repository can now be packaged...

  18. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ... as AAV5. Find the AAV prep here The red-shifted calcium sensor jRGECO1a is now available as AAV-PHPeb...mRNA display (FingRs), Xue Han’s lab has developed AAV vectors to selectively visualize excitatory and inhibitory...AAV-PHPeb. Find the AAV prep here ...

  19. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ...action. AAV: A Versatile Viral Tool for Gene Expression in Mammals Adeno-associated viruses (AAVs) are commonly...Cepko’s Lab at Harvard Medical School describes how AAVs function and indicates important things to consider...

  20. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ...behaviors. Find dopamine and other biosensors in our AAV vector inventory. References Darvesh, Altaf S.,...viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources ...service Find viral protocols and videos Find biosensor AAVs for your research ...
Showing: 81 - 100 of 202 results