We narrowed to 202 results for: aav
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TypeBlog PostPublishedMay 21, 2020, 1:15 p.m....restraint include an AAV-based sparse labeling system where a limiting amount of AAV are injected into the...However, this system requires precise titration of the AAV. So far, tools to overcome this challenge in Drosophila...
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Meet the New Addgene Board Members!
TypeBlog PostPublishedApril 28, 2020, 1:14 p.m....project to develop an AAV-based COVID-19 vaccine. Vandenberghe’s work with AAVs led to many interactions...was compelled to be part of that.” He’s deposited AAV constructs for gene delivery. In 2018, he interviewed... -
Addgene's Top 10 Blog Posts of 2015
TypeBlog PostPublishedDec. 7, 2015, 6 p.m....Match Made in Heaven: CRISPR/Cas9 and AAV Adeno-associated viral (AAV) vectors are useful for in vivo gene...the clever ways to successfully combine CRISPR and AAVs. 9. CRISPR 101: Validating Your Genome Edit Once... -
8 Great Depositor Research Stories
TypeBlog PostPublishedDec. 15, 2022, 2:15 p.m....a CRISPR/dCas9-based tool for DNA demethylation. AAV Q&A with Tim Miles: While not technically a research... excellent Q&A from Tim Miles on everything from AAV prep to targeting to animal models. It was one of... -
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons
TypeBlog PostPublishedApril 24, 2015, 2:39 p.m....adeno-associated viral vectors (AAV) available - AAV-EF1a-BbChT and AAV-EF1a-BbTagBY. These constructs ...associated with AAV; co-infection with both constructs produces a minimum of 8 colors. The use of AAV provides... -
Viral Vectors 101: Transductions
TypeBlog PostPublishedApril 30, 2024, 1:15 p.m....non-integrating (adenovirus and AAV) but can still express episomally long-term (AAV). If you perform a lipid-based...with: either retrovirus, lentivirus, adenovirus, or AAV. Then, your desired DNA to be integrated can be cloned... -
New Viral Vectors - Summer 2024
TypeBlog PostPublishedJuly 9, 2024, 1:15 p.m....viral service pAAV_hSyn1_nLightR AAV9 Biosensors Patriarchi New viral service pGP-AAV-syn-jGCaMP8m-WPRE... Data Hub What is a Virus (and a Viral Vector)? AAV Variables that Matter...serotypes pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2] AAV5, AAV8 Optogenetics Boyden New serotypes pAAV-CAG-...serotype pAAV-hSyn-GRAB_OT1.0 AAV1, AAV9 Biosensors Li New viral service with multiple serotypes pAAV-hSyn-GRAB_OTmut...Category Depositor Notes pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE AAV1, AAV5 Optogenetics Yizhar New...with multiple serotypes pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE AAV1, AAV5 Optogenetics Yizhar New viral...service with multiple serotypes pAAV_hSyn-PdCO-EGFP-WPRE AAV1, AAV5 Optogenetics Yizhar New viral service... -
New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor
TypeBlog PostPublishedApril 7, 2022, 1:15 p.m....variants come in AAV packaged virus aliquots being deposited at Addgene. Other plasmids and pAAVs can be ordered...protein CAG: Generic strong promoter. Formulated as AAV-DJ for neuronal expression. CAG-FLEX: CAG promoter... Biosensors Browse All Fluorescent Proteins Find AAV Tools References Marvin, Jonathan S., et al. “An... -
Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection
TypeBlog PostPublishedJune 25, 2019, 12:54 p.m....lentiviral and Adeno Associated Viruses (AAV). Lentiviral and AAV can be used to create both transient cell...You can find all kinds of different lentiviral and AAV plasmids as well as ready-to-use viral preparations... -
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics
TypeBlog PostPublishedMarch 3, 2020, 2:15 p.m....here. AAV delivery of split base editors Article contributed by Jennifer Tsang AAVs are a great ...transduced at levels similar to that of a single AAV expressing GFP. The lab tested the base editors in... -
Plasmids 101: FLEx Vectors
TypeBlog PostPublishedApril 28, 2016, 2:30 p.m....improvement of optogenetic adeno-associated virus (AAV) vectors. Listed below are a few additional uses ...Since most optogenetic reporters are delivered using AAV injection, the majority of the cells surrounding ...about Other Means of Genome Engineering Using FLEx AAV Vectors? Learn about Viral Vector Elements Read Our... -
Addgene's Top Blog Posts from 2020
TypeBlog PostPublishedDec. 29, 2020, 2:15 p.m....details in this blog post. 9. Droplet Digital PCR for AAV Quantitation by Kate Harten DeMaio We made the switch... switch to droplet digital PCR to quantify AAV titers. Get the details on the methods in this blog post... -
Chemogenetics vs. Optogenetics: Which Method Should I Choose?
TypeBlog PostPublishedJune 11, 2020, 1:34 p.m....cells, commonly through viral delivery by AAV. You can find AAV tools for optogenetics and chemogenetics... -
Deciphering the Mysteries of Behavior with Viral Vectors
TypeBlog PostPublishedSept. 17, 2019, 12:40 p.m....diseases. The researchers injected Cre-expressing AAV-5 into the DRN of a transgenic mouse harbouring a...Addgene has several recombinase-expressing ready-made AAV vectors which can be used in numerous floxed animal...viral service Find viral protocols and videos Find AAVs for your research ...vigilance. A recent study investigated the injection of AAV8 particles expressing an activating chemogentic receptor...specifically activate the Drd1 neurons, they injected AAV2 particles into a Drd1-Cre transgenic mouse to deliver... -
Beginner's Guide to Viral Vectors
TypeBlog PostPublishedJune 2, 2017, 2:30 p.m....important characteristics of retroviruses, lentiviruses, AAVs, and adenoviruses in this easy-to-use guide. Print... -
Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight
TypeBlog PostPublishedJuly 16, 2019, 12:59 p.m.... viral service Find optogenetics AAVs for your research Find all AAVs from the viral service ... -
Viral Vectors 101: What is a Virus (and a Viral Vector)?
TypeBlog PostPublishedAug. 18, 2023, 4:15 p.m....are named after the viruses they’re derived from: AAVs, lentivirus, gamma retrovirus, and adenovirus. And... Vector Elements More resources from Addgene The AAV Data Hub Addgene's Viral Services Addgene's Viral... -
Viral Vectors 101 eBook 2nd Edition
TypeBlog PostPublishedJuly 11, 2024, 1:15 p.m....producing your own vectors in the lab to optimizing AAVs in your experiment. Our second edition includes... -
The PAM Requirement and Expanding CRISPR Beyond SpCas9
TypeBlog PostPublishedAug. 20, 2020, 2:30 p.m.... it can be packaged into adeno-associated virus (AAV). At 984 amino acids in length, Cas9 from Campylobacter... smaller than SaCas9 and is also compatible with AAV delivery. NmCas9, another small ortholog, displays... Read more about Cpf1 Read more about SaCas9 and AAV Check out our CRISPR Featured Topic Page Resources... -
Hot Plasmids and Viral Preps - September 2021
TypeBlog PostPublishedSept. 21, 2021, 1:15 p.m....available including: pAAV-hsyn-GRAB_DA2h in AAV9 New GRAB-DA and 5HT sensors New AAV tools to selectively...from the Dimidschstein Lab More GCaMP8 ready-to-use AAV preps ...