We narrowed to 371 results for: crispr

Learn how to design a CRISPR screening experiment targeting enhancers.

Aug. 9, 2016, 2:30 p.m.

Fragmid is a quick, easy way to design and create CRISPR plasmids. Read about the Doench lab's new modular cloning-based approach in this blog post!

Aug. 29, 2024, 1:15 p.m.

A review of alternative PAM and PAM-flexible Cas enzymes.

March 7, 2024, 2:39 p.m.

Nanoblades are a CRISPR delivery tool. These Nanoblades are engineered MLV-like particles loaded with Cas9-gRNA ribonucleoproteins.

Sept. 26, 2019, 12:50 p.m.

Learn more about non-homologous end joining (NHEJ) and how it relates to genome engineering technologies such as CRISPR/Cas9.

April 16, 2015, 3:45 p.m.

The CRISPR babies saga shows us that we need action to move forward in bringing CRISPR technologies from bench to bedside.

Dec. 20, 2018, 1:15 p.m.

This post describes CRISPR-derived base editors to convert cytosine to adenine or guanine, called CGBEs or GBEs.

March 25, 2025, 1:15 p.m.

Learn about new RNA-targeting Cas13d enzymes that average only 2.8 kb in size, are easy to package in low-capacity vectors, have high on-target and low off-target editing rates.

May 3, 2018, 1:48 p.m.

A new CRISPR/Cas9 system allows genome editing in all E. coli strains.

April 13, 2021, 1:15 p.m.

Baby KJ was treated for his devastating genetic disorder with CRISPR base editing. This post describes the prior advances that made his cure possible.

Sept. 16, 2025, 1:15 p.m.

Learn how you can use HDR and CRISPR to make a knock-in cell line for your research.

Dec. 22, 2022, 2:15 p.m.

Choosing a CRISPR activator? This article features four of the most popular CRISPR activators (dCas9-VP64, SAM, SunTag, and VPR) and some newer activators like dCas9-CBP and SPH.

Oct. 6, 2020, 1:15 p.m.

Mobile-CRISPRi is a set of modular CRISPRi tools that can be used in diverse bacteria from various environments. It has the advantage of stable genomic integration and is easily transferred via conjugation.

April 4, 2019, 12:53 p.m.

Genome-wide CRISPR/Cas9 screens are a high-throughput systematic approach for identifying genes involved in a biological process. Recently the Bassik Lab at Stanford developed a new genome-wide CRISPR knockout screen to analyze unanswered questions about CRISPR screen design by including safe targeting guides and short gRNAs.

Sept. 13, 2018, 1:55 p.m.

The development of adenoviral vectors for the delivery of CRISPR/Cas9 expands genome engineering to primary cells.

Dec. 10, 2020, 1 p.m.

New CRISPR pooled libraries from the Broad Institute allow you to specifically target the mouse and human kinomes in your next screening experiment.

Sept. 9, 2016, 2:43 p.m.

Rationally engineered Cas9 variants eSpCas9 SpCas9-HF1 enhance CRISPR on-target specificity. Learn how in this post.

Dec. 16, 2015, 3:30 p.m.

A new open source tool for CRISPR experiments, CrispyCrunch, has been developed by the Chan Zuckerberg Initiative to design batch gRNAs, templates, and sequencing primers for CRISPR + HDR experiments.

Feb. 7, 2019, 2:16 p.m.

With the limitations of qRT-PCR based COVID-19 testing, scientists develop CRISPR-based diagnostic tools for COVID-19.

May 5, 2020, 1:15 p.m.

This post discusses the applications, advantages, and caveats to using hybrid guide RNAs, in which DNA bases are substituted for RNA bases.

Jan. 27, 2026, 2:14 p.m.