We narrowed to 192 results for: gfp

1. pSiM24: Simplifying Plant Genetic Engineering

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   Blog Post

   Published

   Sept. 29, 2015, 2:30 p.m.

   ...Arabidopsis, and corn. For both β-glucuronidase (GUS) and GFP reporters, as well as multiple transient and transgenic...

2. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

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   Blog Post

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   July 29, 2014, 6:16 p.m.

   ...from Joachim Goedhart: Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection.  ...sun, from the standard oldies but goodies (e.g., EGFP and YFP) to the new and improved fruit colors (e.g...

3. Tag Your Favorite Yeast Genes with Ease

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   Blog Post

   Published

   Nov. 19, 2013, 2:37 p.m.

   ...either the N- or C-terminus (3xHA, 13xMyc, GST, or GFP). Longtine et al.(3) describe a complimentary set...

4. Quick Guide to All Things Lentivirus

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   Blog Post

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   March 21, 2017, 2:30 p.m.

   ...containing the gene of interest (pTet-IRES-EGFP, pPRIME-Tet-GFP-FF3) and one with either tTA or rtTA (FUW-M2rtTA...viral titre to the number of cells transduced by a GFP encoded virus, and the reverse transcriptase activity...

5. Antibodies 101: Introduction to Antibodies

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   Blog Post

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   Jan. 19, 2021, 2:15 p.m.

   ...blot showing GFP expression in cells that have been transfected with a plasmid encoding GFP. Actin, a common...

6. Viral Vectors 101: Parts of the AAV Transfer Plasmid

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   Blog Post

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   Aug. 11, 2020, 1:15 p.m.

   ...or smaller. Some examples include a reporter like GFP, some smaller CRISPR-Cas proteins such as SaCas9,...

7. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

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   Blog Post

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   May 21, 2020, 1:15 p.m.

   ...transcription activation systems, such as LexA/p65 or mCD8/GFP, to allow sparse labeling in a variety of fly cell...

8. Plasmids 101: What is a plasmid?

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   Blog Post

   Published

   April 2, 2020, 6:17 p.m.

   ...cell Plasmids 101: Green Fluorescent Protein (GFP) Plasmids 101: Luciferase Monitor the level of...

9. CRISPR Challenges: Standardization and Homology Directed Repair

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   Blog Post

   Published

   Feb. 14, 2018, 2:36 p.m.

   ...PubMed Central PMCID: PMC5495063 Kan et al. use a GFP-BFP conversion system to assess precision genome ...

10. Hot Plasmids - October 2022

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    Blog Post

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    Oct. 6, 2022, 1:15 p.m.

    ...Addgene’s western blot for the myc-tagged protein GFP-smFP-myc expressed from Plasmid 98926. See the Applications...

11. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type

    Blog Post

    Published

    June 17, 2021, 1:17 p.m.

    ...strain transformed with pBLADE expressing superfolder GFP instead of mCherry under the PBAD promoter. They ...

12. New Viral Vectors - Winter 2025

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    Blog Post

    Published

    Jan. 28, 2025, noon

    ...serotype (multiple) rAAV-CAG::FLEX-rev:: hM4D-2a-GFP AAV1 Chemogenetics Scott Sternson New viral prep ...Recombinase Ian Wickersham New viral prep pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 AAV2 Recombinase James M. ...Recombinase James M. Wilson New serotype pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 AAVrg Recombinase James M... prep AiP14033: pAAV-AiE0779m_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN4033) AAV-PHPeB Optogenetics... prep AiP14035: pAAV-AiE0452h_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN4035) AAV-PHPeB Optogenetics... prep AiP15140: pAAV-AiE0873m_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN5140) AAV-PHPeB Optogenetics...

13. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

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    Blog Post

    Published

    July 18, 2017, 12:41 p.m.

    ...using a bacterial expression system such as the pET GFP LIC cloning vector and the pOKD plasmids. The recombinant...

14. New Norepinephrine Indicators: nLightG and nLightR

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    Blog Post

    Published

    Dec. 12, 2023, 2:15 p.m.

    ...contains circularly permuted green fluorescent protein (GFP), and nLightR uses a red fluorescent protein, mApple...

15. Antibodies 101: Affinity Tags

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    Blog Post

    Published

    Sept. 19, 2023, 1 p.m.

    ... (FLAG, Myc, etc.) and fluorescent protein tags (GFP, mCherry, etc.). Fluorescent tags are primarily used...

16. Year of the Camelid: Antibody Style

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    Blog Post

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    May 9, 2024, 1:15 p.m.

    ...highlighted back in 2015, where sdAbs were used to create GFP scaffolds for transcriptional activation.  sdAbs ...

17. 10 Great Guest Posts We're Thankful For

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    Blog Post

    Published

    Nov. 18, 2016, 3 p.m.

    ...Favorite Fluorescent Protein Oligomerizes in Cells GFP oligomerization can lead to misleading artefacts ...

18. Using Addgene's New Viral Service for Your Research

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    Blog Post

    Published

    Nov. 17, 2016, 3:30 p.m.

    ...also have lentivirus for delivering control shRNAs, GFP, and rtTA from the Tet-on system. The first two can...

19. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

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    Blog Post

    Published

    Dec. 13, 2018, 1:14 p.m.

    ...endocytosis of a fluorescent dye and transduction of GFP-expressing AAV particles we did not observe any difference...

20. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type

    Blog Post

    Published

    Sept. 15, 2015, 2:30 p.m.

    ... University with David Goldstein’s lab, will use GFP-ATP1A3, FLAG-ATP1A3, and ATP1B3 plasmids to explore...