We narrowed to 202 results for: aav
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TypeBlog PostPublishedJan. 9, 2018, 3:02 p.m....interneurons, Gordon Fishell’s lab has developed a new AAV approach using regulatory elements that restrict ...to this cell type. To this end, they constructed AAV plasmids bearing one of the distal-less homeobox ...these tools in virtually any vertebrate. This novel AAV approach allows you to target and functionally manipulate...tool for the research community. You can find the AAV vectors for infecting GABA-ergic interneurons here...27798629 Listen to Our Podcast Segment on This New AAV Tool mCyRFP1: Novel fluorescent protein that...
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How Addgene Creates Content
TypeBlog PostPublishedNov. 17, 2022, 2:15 p.m....Addgene’s mission statement extends beyond plasmids, AAVs, and antibodies to our content, website, Data Hub...enable that process. Our repository of plasmids, AAVs, antibodies, and data allows scientists to easily... -
Early Career Researcher Toolbox: Social Media for Scientists
TypeBlog PostPublishedDec. 11, 2018, 12:46 p.m....and shared the paper with my lab since one of the AAV vectors in the paper seemed like it could help one...my lab mate’s with her project. Unfortunately the AAV didn’t give us the results we hoped for, but it did... -
Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering
TypeBlog PostPublishedMay 3, 2018, 1:48 p.m....then easy to package in low-capacity vectors like AAV, a popular vector due to its low immunogenicity. ...acceptor and two putative splice enhancers. After AAV-mediated delivery to iPS-derived cortical neurons... -
Plasmids 101: Cre-lox
TypeBlog PostPublishedJan. 13, 2015, 3:47 p.m....Additionally, Cre-containing adenovirus (Ad-Cre) or AAV (AAV-pgk-Cre) has been used to successfully introduce... -
Hot Plasmids: Summer 2024
TypeBlog PostPublishedAug. 27, 2024, 1:15 p.m....proteins than SpyCas9, allowing them to be packaged in AAV vectors, potentially with multiplexed targets. ...from Neumann et al. 2024. The team showed that AAV-delivered CHARM could silence the expression of prion... (2024). Brainwide silencing of prion protein by AAV-mediated delivery of an engineered compact epigenetic... -
Treating Muscular Dystrophy with CRISPR Gene Editing
TypeBlog PostPublishedJan. 26, 2016, 3:30 p.m....culture, but Ran et al. have shown that CRISPR and AAV can be used together for postnatal genome editing...facets of CRISPR DMD therapy. Long et al. show that AAV-CRISPR does not cause obvious germline modification...especially stem cells 2. Remove any immunogenicity of the AAV vector Once delivery has been optimized, it’s important...with AAV9, whereas Nelson et al. and Tabebordbar et al. used the shorter SaCas9 with AAV8 and AAV9, respectively... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog PostPublishedMay 11, 2021, 1:15 p.m....the protein of interest. In our studies, we used AAV viral vectors to express hM4Di in neurons of rhesus...years ago (Field et al., 1988). Thus, we injected AAVs carrying hM4Di-HA in the brains of mice and monkeys... -
Addgene: The First Twenty Years
TypeBlog PostPublishedDec. 19, 2024, 2:15 p.m....vectors from some of our most popular lentiviral and AAV vectors, as well as developing and sharing supportive...be expanding our viral vectors service to include AAV Packaged on Request! “I love Addgene. I think resources... -
Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP
TypeBlog PostPublishedNov. 24, 2015, 3:30 p.m....and 100% of DsRed+ cells also expressed GFP. Using AAV constructs, Tang et al. found that Cre-DOG could ...optogenetic studies, and since cells infected with AAV-Cre-DOG retain normal neuronal function, this system... -
Export Control Regulations Lifted for Plasmids Containing Rabies and VSV-G Genes
TypeBlog PostPublishedFeb. 6, 2025, 2:15 p.m....Tracing” blog post! You can also browse tools for AAV tracing, made from rabies plasmids deposited by Ian... -
Genome Engineering, CRISPR, and Research in Singapore: Interview with Wei Leong Chew
TypeBlog PostPublishedFeb. 6, 2018, 2 p.m.... lab. Genome engineering, gene delivery methods, AAVs, and more. Chapter 7/Outro: Advice for researchers... -
Making CRISPR Plasmids Using Fragmid
TypeBlog PostPublishedAug. 29, 2024, 1:15 p.m....CMV-driven; CROPseq) Mammalian AAV pRDA_889 (ITRs from AAV2) Mammalian Transposon pRDA_...Mammalian or drosophila) Delivery type (lentivirus, AAV, transposon, Plasmid) Destination vector (dropdown... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog PostPublishedMarch 8, 2017, 3:15 p.m....expression, bacterial expression, lentiviral, retroviral, AAV, RNAi, luciferase, cre/lox, yeast expression, worm...in bacteria) such as lentiviral, retroviral, and AAV plasmids, choose the NEB Stable strain. For plasmids... -
Expanded Access to Rabies Plasmids
TypeBlog PostPublishedFeb. 1, 2024, 2:15 p.m....Tracing” blog post! You can also browse tools for AAV tracing, made from rabies plasmids deposited by Ian... -
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!
TypeBlog PostPublishedDec. 3, 2019, 11:38 a.m....experiment involves delivering a calcium biosensor using AAV, you might decide you’d really prefer to order ready-to-use...promoter for your target cell type Serotype: Choose the AAV capsid or lentiviral prep needed Tags: Identify a... -
New CRISPR Tools: Cas7-11 and PASTE
TypeBlog PostPublishedFeb. 17, 2022, 2 p.m....systems have enzymes small enough to fit into a dual AAV system, albeit with lower knockdown efficiencies....HITI and HDR. PASTE is also compatible with both AAV and adenovirus templates. The system, which is larger... -
Hot Plasmids - November 2023
TypeBlog PostPublishedNov. 14, 2023, 3:35 a.m.... ideal for size-limited AAV delivery. PE6c and PE6d are optimized for dual-AAV systems. These variants... -
Summer Fun at Addgene!
TypeBlog PostPublishedMay 12, 2022, 1:15 p.m....Navigating an Expanding Landscape for Engineered Systemic AAVs” with the director of CalTech’s CLOVER lab, Tim ... -
Editor's Choice, July 2016
TypeBlog PostPublishedAug. 5, 2016, 3 p.m....Gendered Innovations: Why Does Sex of the Cell Matter? AAV: A Versatile Viral Tool for Gene Expression in Mammals...