We narrowed to 376 results for: crispr

Fourteen years after Cas9, CRISPR has moved beyond DNA cutting. Read Addgene's recap of CRISPR Frontiers 2026, covering programmable gene insertion, RNA-sensing cell killing, and next-gen base and prime editors.

Aug. 25, 2026, 1:15 p.m.

This post discusses the applications, advantages, and caveats to using hybrid guide RNAs, in which DNA bases are substituted for RNA bases.

Jan. 27, 2026, 2:14 p.m.

The Framework for In vivo T cell Screens (FITS) allows for in vivo CRISPR screening in T cells. Learn more about FITS in our blog post.

May 2, 2024, 1:15 p.m.

Learn how to use Cas9 nickases for genome editing, including homology directed repair.

March 15, 2018, 12:59 p.m.

Laura Drepanos from the Broad Institute shares the strategies her team used to optimize the CRISPR knockout libraries Jacquere and Julianna.

Sept. 8, 2026, 1:14 p.m.

Cas14 was discovered by the Doudna lab. It cleaves ssDNA (but not dsDNA) in trans and can be adapted for a diagnostic toolkit such as DETECTR.

Nov. 29, 2018, 1:53 p.m.

An easy demethylation protocol uses CRISPR-based dCas9-directed DNA avoids the use of Tet

Dec. 14, 2021, 2:15 p.m.

This post describes CRISPR-derived base editors that catalyze base transversions, such as purines like A or G to pyrimidines like T or C.

April 1, 2025, 1:15 p.m.

Learn about xCas9, an engineering Cas9 variant with broad PAM flexibility and high on-target specificity.

March 28, 2018, 6:52 p.m.

Learn all about new plasmids available at Addgene including an anti-CRISPR optogenetic tool, the fluorescent voltage indicator Archon1, photoswitchable proteins, and a simplified 2in1 cloning method.

March 19, 2019, 1:31 p.m.

High schoolers invented a new diagnostic for Lyme disease. In the process, they also created a tool for jointly designing RPA primers and Cas12a sgRNAs.

May 12, 2026, 1:15 p.m.

Prime editing is a search-and-replace CRISPR editing method that offers versatility and precision in genome editing. We cover common prime editing tools.

Aug. 11, 2026, 1:15 p.m.

Learn about new plasmids including optogenetic control of synaptic transmission, acoustic reporter genes, miRNA reporter vectors, and CRISPR-Cas9 delivery for in vivo screening.

June 6, 2019, 12:41 p.m.

Use CRISPRainbow to label multiple genome sites with fluorescent proteins of various colors.

Feb. 28, 2017, 3:30 p.m.

Learn about new plasmids at Addgene including CRISPR, auxin inducible degradation, MerMAID channelrhodopsins, and genome looping.

Sept. 3, 2019, 1:15 p.m.

Learn about new plasmids at Addgene including plasmids for CRISPR, sparse cell labeling, reporting calcineurin activity, and an alternative to DNA staining dyes.

June 2, 2020, 1:15 p.m.

Learn about CRISPR activation tools for neuroscience including a SPH transgenic mice, neuron-optimized viral vectors, and high-throughput screening.

July 2, 2019, 12:51 p.m.

Learn how you can use adeno-associated viruses (AAV) for genome editing - whether combined with nucleases or not. Also, read about the advantages and disadvantages of using AAV vs. CRISPR.

March 27, 2018, 1:32 p.m.

CRISPR genome editing often proceeds through NHEJ or HDR but microhomology-mediated end-joining (MMEJ) has many benefits - read about them here.

Feb. 23, 2016, 3:30 p.m.

Learn how Cpf1 stacks up against Cas9 and NgAgo

July 14, 2016, 2:30 p.m.