We narrowed to 371 results for: crispr

The Framework for In vivo T cell Screens (FITS) allows for in vivo CRISPR screening in T cells. Learn more about FITS in our blog post.

May 2, 2024, 1:15 p.m.

Learn how to use Cas9 nickases for genome editing, including homology directed repair.

March 15, 2018, 12:59 p.m.

Cas14 was discovered by the Doudna lab. It cleaves ssDNA (but not dsDNA) in trans and can be adapted for a diagnostic toolkit such as DETECTR.

Nov. 29, 2018, 1:53 p.m.

An easy demethylation protocol uses CRISPR-based dCas9-directed DNA avoids the use of Tet

Dec. 14, 2021, 2:15 p.m.

This post describes CRISPR-derived base editors that catalyze base transversions, such as purines like A or G to pyrimidines like T or C.

April 1, 2025, 1:15 p.m.

Prime editing is a CRISPR/Cas9 editing method that offers more versatility and precision to genome editing. This post covers common prime editing tools.

Jan. 13, 2025, 9:49 p.m.

Learn about xCas9, an engineering Cas9 variant with broad PAM flexibility and high on-target specificity.

March 28, 2018, 6:52 p.m.

Learn all about new plasmids available at Addgene including an anti-CRISPR optogenetic tool, the fluorescent voltage indicator Archon1, photoswitchable proteins, and a simplified 2in1 cloning method.

March 19, 2019, 1:31 p.m.

High schoolers invented a new diagnostic for Lyme disease. In the process, they also created a tool for jointly designing RPA primers and Cas12a sgRNAs.

May 12, 2026, 1:15 p.m.

Learn about new plasmids including optogenetic control of synaptic transmission, acoustic reporter genes, miRNA reporter vectors, and CRISPR-Cas9 delivery for in vivo screening.

June 6, 2019, 12:41 p.m.

Use CRISPRainbow to label multiple genome sites with fluorescent proteins of various colors.

Feb. 28, 2017, 3:30 p.m.

Learn about new plasmids at Addgene including CRISPR, auxin inducible degradation, MerMAID channelrhodopsins, and genome looping.

Sept. 3, 2019, 1:15 p.m.

Learn about new plasmids at Addgene including plasmids for CRISPR, sparse cell labeling, reporting calcineurin activity, and an alternative to DNA staining dyes.

June 2, 2020, 1:15 p.m.

Learn about CRISPR activation tools for neuroscience including a SPH transgenic mice, neuron-optimized viral vectors, and high-throughput screening.

July 2, 2019, 12:51 p.m.

Learn how you can use adeno-associated viruses (AAV) for genome editing - whether combined with nucleases or not. Also, read about the advantages and disadvantages of using AAV vs. CRISPR.

March 27, 2018, 1:32 p.m.

CRISPR genome editing often proceeds through NHEJ or HDR but microhomology-mediated end-joining (MMEJ) has many benefits - read about them here.

Feb. 23, 2016, 3:30 p.m.

Learn how Cpf1 stacks up against Cas9 and NgAgo

July 14, 2016, 2:30 p.m.

The Doudna lab recently described a new, thermostable Cas9 variant, GeoCas9. Learn how you can use GeoCas9 in your next genome engineering experimetn.

Sept. 14, 2017, 12:40 p.m.

Find out how you can use new CRISPR tagging plasmids from the Allen Institute for Cell Science to tag your cellular structure of interest.

April 6, 2017, 1:02 p.m.

Guest blogger Pooran Dewari presents results of an NgAgo survey he gave to some of the first NgAgo users. Find out how NgAgo compares to Cas9 so far.

Aug. 4, 2016, 2:30 p.m.