We narrowed to 161 results for: grna

Learn about the basic elements that make up the AAV transfer plasmid, including the ITRs, transgene, promoter, and other cis-regulatory elements.

Aug. 11, 2020, 1:15 p.m.

Having trouble with viral vectors? Here are several tips to help prevent recombination in viral vectors or other plasmids with repeating elements.

Sept. 14, 2021, 1:15 p.m.

Learn about new plasmids at Addgene including CRISPR, auxin inducible degradation, MerMAID channelrhodopsins, and genome looping.

Sept. 3, 2019, 1:15 p.m.

Learn how to use and apply NEBuilder HiFi DNA Assembly Technology from New England Biolabs for quick and accurate plasmid cloning.

Sept. 6, 2017, 1:01 p.m.

This post describes CRISPR-derived base editors to convert cytosine to adenine or guanine, called CGBEs or GBEs.

March 25, 2025, 1:15 p.m.

It can be difficult to choose the best CRISPR software tool. The CRISPR Software Matchmaker makes choosing a guide RNA design software easier.

Nov. 3, 2015, 3:30 p.m.

A general overview of how and when to use screens vs. selections and the ins and outs of each method.

Nov. 10, 2022, 2:15 p.m.

With the addition of ABE8, a faster and improved adenine base editor, scientists can now make base edits more efficiently and in more contexts.

March 17, 2020, 1:32 p.m.

We've partnered with SnapGene to update our plasmid maps. Learn how to use our new maps to accelerate your research.

June 29, 2017, 1:06 p.m.

Guest blogger Kate Palozola gives some tips on how to keep a good and organized lab notebook for bioinformatic analyses.

June 2, 2016, 2:30 p.m.

Comparing and contrasting Cas9 to the other genome editing-capable Cas family proteins.

Dec. 8, 2022, 2:15 p.m.

Learn about new RNA-targeting Cas13d enzymes that average only 2.8 kb in size, are easy to package in low-capacity vectors, have high on-target and low off-target editing rates.

May 3, 2018, 1:48 p.m.

Recent highlights from Addgene's collection include tools for epigenetic engineering, tracking cell-cell interactions, and a new auxin inducible degron system for C. elegans.

March 23, 2021, 1:15 p.m.

This round-up of new plasmids from Addgene include blue-light inducible Cre for use in bacteria, CRISPR-Casɸ mGold, and new CRISPR plasmids and viral preps.

Jan. 5, 2021, 2:15 p.m.

Gamma-retroviral vectors and lentiviral vectors can be used in a wide variety of experiments including those involving genome engineering, RNAi, and inducible gene expression. In this post, learn about some of the most popular viral vectors available from Addgene and their uses.

July 24, 2018, 12:51 p.m.

Learn how to use the dTAG system to inducibly degrade your protein of interest.

June 21, 2018, 2:06 p.m.

All new plasmids deposited at Addgene are verified by next-generation sequencing to provide you with more data to choose the right plasmid for your next experiment.

April 6, 2021, 12:30 p.m.

Researchers inactivate genes to investigate their function. This article describes techniques/plasmids used to knockout and knock-in specific genes in mammalian cells.

Dec. 1, 2016, 3:30 p.m.

Guest blogger Pooran Dewari presents results of an NgAgo survey he gave to some of the first NgAgo users. Find out how NgAgo compares to Cas9 so far.

Aug. 4, 2016, 2:30 p.m.

Check out the latest Hot Plasmids, viral vectors, and antibodies in the Addgene repository!

Aug. 28, 2025, 1:15 p.m.