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We narrowed to 181 results for: grna

Showing: 121 - 140 of 181 results

  1. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...Addgene.org Browse Our CRISPR Resources Find Validated gRNAs Browse All Plasmids ...thousands of base pairs. Cloning sgRNAs Generating constructs to produce sgRNA for CRISPR experiments can be... plating, screening and generation of sgRNA. Generation of sgRNA libraries, with maximum complexity, is...constructs with multiple point mutations Cloning sgRNAs Production of Yeast Expression Cassettes Generating...synthetic oligo to generate a single-guide RNA (sgRNA). Using only one single-stranded oligo, instead ...to create a double-stranded oligo. Generating an sgRNA construct becomes as simple as combining your favorite...

  3. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ... Acronym Full name Notes CRISPR terms gRNA/sgRNA Guide RNA RNA used by Cas9 to locate a matching... the DNA strand that does not base pair with the gRNA. Base editors target this “non-target strand.” To... predicting CGBE editing outcomes and assisting sgRNA selection is available at http://www.sunlab.fun:...

  4. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    Published
    Nov. 3, 2015, 3:30 p.m.
    ...Resources at Addgene Get Tips on How to Design a gRNA Read Our CRISPR Guide to Catch up on All the CRISPR...Read Other Blog Posts about CRISPR Find validated gRNAs ...of what determines good sgRNA design. Incidentally, the first software for sgRNA design (by Hsu et al.,...technical expertise, thoughtful single guide RNA (sgRNA) design, and the abnormally low GC content of the...without computational support. Indeed, rational sgRNA design is not possible without relying on some kind...end of 2014, I began developing software to make sgRNA design accessible to all. At the time I thought ...  Breaking down the barriers: My view on current sgRNA design tools The goal of this post is to provide...

  5. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ..., genome-wide knockout screens are possible with gRNA libraries targeting all known genes. This is a perfect...

  9. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...good reason. It is guided by an RNA molecule, the gRNA, to a complementary site in the genome where it ...

  10. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ... system developed by Taekjip Ha’s lab where Cas9-gRNA complex binds the target DNA without cleavage until...

  14. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...CRISPR plasmids for protein tagging Find validated gRNAs ...

  15. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...interest is difficult to target specifically with a gRNA). Be sure to keep these techniques in mind when ...

  16. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...nickase) fused to a deaminase that makes the edit A gRNA targeting Cas to a specific locus A target base ...CRISPR plasmids for base editing Find validated gRNAs ...

  20. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...edit to be made when multiple As are present in the gRNA spacer, as ADAR will preferentially edit an adenine...
Showing: 121 - 140 of 181 results